Interaction and tolerance to Alternaria brassicae in Indian mustard (Brassica juncea) genotypes

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  • P D MEENA Directorate of Rapeseed-Mustard Research, Bharatpur-321 303, Rajasthan
  • MANJU BALA Directorate of Rapeseed-Mustard Research, Bharatpur-321 303, Rajasthan
  • PANKAJ SHARMA Directorate of Rapeseed-Mustard Research, Bharatpur-321 303, Rajasthan
  • DHIRAJ SINGH Directorate of Rapeseed-Mustard Research, Bharatpur-321 303, Rajasthan


Alternaria blight, Biochemical changes, Genotypes, Indian mustard


The biochemical changes were observed in leaves of Indian mustard tolerant (EC-399301, EC-399296, EC-399299, PHR-2) and susceptible (Varuna) genotypes infected with Alternaria blight (AB) caused by Alternaria brassicae at critical stage (75 days after sowing) of disease development. The presence of metabolites in infected B. juncea leaves such as glucosinolate (µ moles), phenol (mg/g), sugar (mg/g), protein (mg/g), carotenoids (µg/g) and chlorophyll (mg/g) were highly associated with the fungal infection. As compared to the susceptible check Varuna, level of glucosinolate, sugar, carotenoids and chlorophyll were decreased in tolerant hosts' leaves after pathogen infection. Although, the phenol and protein content in tolerant and susceptible genotypes were increased in AB infected leaves than healthy leaves. Our findings are discussed within the context of A. brassicae-B. juncea interaction and tolerance to this pathogen. The results indicated that factors conditioning the host response to A. brassicae might be the outcome of complex biochemical changes operated in host genotypes.


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Arnon D I 1949. Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiology, 24:1-15.

Baraka M A, Ghonim M I, Abdel N H M and Ahmed M I 2004. Biochemical mechanisms of resistance in some bean cultivars against charcol rot disease. Bulletin of Faculty of Agriculture, Cairo University, 55: 97-120.

Bhargava P K and Khare M N 1988. Factors influencing mechanism of resistance in Alternaria leaf blight of chickpea. Indian Phytopathology, 25: 231-235.

Borah R C, Bajaj K I and Bhatia I S 1978. Biochemical changes in tea leaves infected with red rust. Phytopathology, 89: 167-180.

Choulwar A B and Datar V V 1991. Physiological studies on Alternaria solani causing early blight of tomato. Journal of Maharashtra Agricultural Universities, 16: 265-266.

Dogan S, Turan P, Dogan M, Arslan O and Alkan M 2007. Partial characterization of peroxidase from the leaves of thymbra plant (Thymbra spicata L. var. spicata). European Food Research Technology, 225: 865-871.

Ghosh L, Alam M S, Ali M R, Alam F and Islam R 2003. Changes in some biochemical parameters of mulberry (Morus sp.) leaves after infected with leaf spot disease. Online Journal of Biological Sciences, 3: 508-514.

Junqueira A, Bedendo I and Pascholati S. 2004. Biochemical changes in corn plants infected by the maize bushy stunt phytoplasma. Physiology and Molecular Plant Pathology, 65: 181-185.

Kolte S J, Awasthi R P and Vishwanath 1987. Assessments of yield losses due to Alternaria blight in rapeseed and mustard. Indian Phytopathology, 40: 209-211.

Lichtenthaler H K and Wellburn W R 1983. Determination of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Society Transactions, 11: 591-592.

Mali P C, Burman U and Lodha S 2000. Effect of planting dates and development of yellow mosaic virus on biochemical constituents of moth bean genotypes. Indian Phytopathology, 53: 379-383.

Meena P D, Meena R L, Chattopadhyay C and Arvind Kumar 2004. Identification of critical stage for disease development and biocontrol of Alternaria blight of Indian mustard (Brassica juncea). Journal of Phytopathology, 152: 204-209.

Mogle I R and Mayee C D 1981. Comparative changes in reducing sugar, starch and free amino acids accompanying downy mildew infection in resistant, moderately resistant and susceptible leaves of pearl millet. Indian Journal of Mycology and Plant Pathology, 2: 61-65.

Nema A G 1991. Changes in chlorophyll, nitrogen, protein, amino acid and some enzyme contents in betelvine leaves infected with Xanthomonas campestris pv. betlicola. Indian Phytopathology, 44: 9-14.

Parashar R D, Sidhan G S and Hooda I 1987. Role of phenolic compounds and carbohydrates in resistance of bajra cultivars to downy mildew. Plant Disease Research, 2: 120-121.

Pati P K, Sharma M, Salar R K, Sharma A, Gupta A P and Singh B 2008. Studies on leaf spot disease of Withania somnifera and its impact on secondary metabolites. Indian Journal of Microbiology, 48: 432-437.

Rathore B S, Pathak V N and Rathore R S 2001. Impact of downy mildew on bio-constituents and rate of transpiration in blond-psyllium plants. Journal of Mycology and Plant Pathology, 31: 258-260.

Saud Z A, Razzaq S, Zaman S and Absar N 2000. Changes in some biochemical parameters and enzyme contents of guava after infected with fruit rot disease. Bangladesh Journal of Biotechnology, 1: 85-90.

Singh H V 2000. Biochemical basis of resistance in Brassica spp. against downy mildew and white rust of mustard. Plant Disease Research, 15: 75-77.

Singh H V 2004. Biochemical transformation in Brassica spp. due to Peronospora parasitica infection. Annals of Plant Protection Sciences, 12: 301-304.

Singh S, Asthir B, Bains N S and Mann S K 2009. Induction of carbohydrate metabolism in relation to leaf blight in barley (Hordeum vulgare). Advances in Biological Research, 3 (3-4): 61-66.

Yadav O P, Yadav T P, Kumar P and Gupta S K 1996. Inheritance of phenols and proteins in relation to white rust (Albugo candida) resistance in Indian mustard. Indian Journal of Genetics, 56: 256-261.

Yamunarani K, Jaganathan R, Bhaskaran R, Govindaraju P and Velazhahan R 2004. Induction of early blight in tomato by Quercus infectoria gall extract in association with accumulation of phenolics and defense related enzymes. Acta Physiologuae Plantarum, 28: 281-290.






How to Cite

P D MEENA, MANJU BALA, PANKAJ SHARMA, & DHIRAJ SINGH. (2014). Interaction and tolerance to Alternaria brassicae in Indian mustard (Brassica juncea) genotypes: INTERACTION AND TOLERANCE TO ALTERNARIA BRASSICAE IN INDIAN MUSTARD GENOTYPES. Journal of Oilseeds Research, 31(2).