Genome-wide association study of tocopherols (vitamin E) in Indian mustard (Brassica juncea)
Genome-wide association study of tocopherols (vitamin E) in Indian mustard (Brassica juncea)
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Authors
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HARJEEVAN KAUR
Dept. of Plant Breeding & Genetics, Punjab Agricultural University, Ludhiana, 141001, India
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SANJULA SHARMA
Dept. of Plant Breeding & Genetics, Punjab Agricultural University, Ludhiana, 141001, India
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JAVED AKHATAR
Dept. of Plant Breeding & Genetics, Punjab Agricultural University, Ludhiana, 141001, India
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JOMIKA DEVI
Dept. of Plant Breeding & Genetics, Punjab Agricultural University, Ludhiana, 141001, India
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CHHAYA ATRI
Dept. of Plant Breeding & Genetics, Punjab Agricultural University, Ludhiana, 141001, India
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S S BANGA
Dept. of Plant Breeding & Genetics, Punjab Agricultural University, Ludhiana, 141001, India
Keywords:
GWAS, Indian mustard, Tocopherols, UPLC, Vitamin EAbstract
A diverse set of 289 mustard accessions was used to establish variation for α-, γ- and total tocopherols in mustard germplasm. A large variation in our diversity panel for tocopherol isomers and total tocopherols. γtocopherols were observed to be more abundant than α-tocopherols in the mustard seeds. This tocopherol variation was used to identify the genetic loci related to tocopherols using GWAS. Various strong candidate genes such as VTE2-2, SAHH2, PP5.2, PES1, ANT1 etc. involved directly or indirectly in the tocopherol biosynthesis pathway were identified through this method.
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References
Bele C, Matea C T, Raducu C, Miresan V and Negrea O (2013) Tocopherol Content in Vegetable Oils Using a Rapid HPLC Fluorescence Detection Method. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 41(1), 93–96.
Mène-Saffrané L (2017) Vitamin E Biosynthesis and Its Regulation in Plants, Antioxidants, 7(2). 10.3390/antiox 7010002.
