Determination of optimal lethal dose for ethyl methane sulphonate (EMS) induced mutagenesis in castor (Ricinus communis L)
Determination of optimal lethal dose for ethyl methane sulphonate (EMS) induced mutagenesis in castor (Ricinus communis L)
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Authors
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P VAMSHI
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana
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P LAKSHMAMMA
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana
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K SADAIAH
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana
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P AKHILA
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana
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T MANJUNATHA
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana
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C LAVANYA
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana
Keywords:
Castor, Chemical mutagenesis, Ethyl Methane SulphonateAbstract
Castor, an indeterminate and perennial non-edible oilseed crop is unsuitable for mechanization over a large scale. Chemical mutagenesis using ethyl methane sulphonate (EMS) is resorted to exploit point mutations and induce monospike or synchronous maturity. In the present study, EMS mutagenesis dosage was standardized using combinations of concentration (0.5 %, 1 % and 1.5%) and duration (4, 8, 12 hours) of EMS treatment along with duration of pre-soaking with water (12 and 24 hours). Data on speed and percent germination of the seed along with root and shoot length at 7 days and 14 days after treatment indicated that 12 hours presoaking followed by 8 hours or 12 hours of treatment with 1.0% EMS treatment is the optimum lethal dose for EMS mutagenesis.
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References
IAEA, Manual on Mutation Breeding. 2018. Austria: Food and Agriculture Organization of the United Nations, International Atomic Energy Agency, 3rd Edition: 51-81.
