Genetic transformation of Trichoderma with GUS and Hyg Genes to study root colonisation in Groundnut

Genetic transformation of Trichoderma with GUS and Hyg Genes to study root colonisation in Groundnut


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Authors

  • M KAVYA Department of Plant Pathology, College of Agriculture, JNKVV, Jabalpur, Madhya Pradesh-482004.
  • R DURGAPRASAD Department of Plant Pathology, College of Agriculture, JNKVV, Jabalpur, Madhya Pradesh-482004.
  • VIBHAPANDEY Department of Plant Pathology, College of Agriculture, JNKVV, Jabalpur, Madhya Pradesh-482004
  • V DINESHKUMAR Department of Plant Pathology, College of Agriculture, JNKVV, Jabalpur, Madhya Pradesh-482004.

https://doi.org/10.56739/jor.v40iSpecialissue.145614

Keywords:

Genetic transformation, Groundnut

Abstract

A stable β-glucoronidase, hygromycin transformed Trichoderma harzianum was obtained by Agrobacterium mediated transformation.The confirmation was made with PCR and gel electrophoresis. This transformed strain was used to study the activity ofTrichoderma in the groundnut root with the aid of βglucoronidase gene expression. Microscopic analysis revealed Trichoderma colonization and the presence of GUSstained mycelium in the root section.

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References

Yang Q, Yang L and Liu PG. Song J. 2007. Agrobacterium tumefaciens mediated transformationof Trichoderma harzianum.KMITL Science and Technology Journal, 7(2).

Yates IE, Hiett KL, kapczynski DR, Smart W, Glenn AE, Hinton DM, Bacon CW, Meinersmann, Liu S and Jaworski AJ. 1999. GUS transformation of the maize fungal endophyte Fusarium moniliforme. MycologyResearch,103 (2):129-136. DOI: https://doi.org/10.1017/S0953756298006911

Journal of Oilseeds Research

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Submitted

2023-11-24

Published

2023-12-14

Issue

Vol. 40 No. Specialissue (2023): Journal of Oilseeds Research

Section

Articles

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The Journal of Oilseeds Research reserves all the copy rights of the published articles and prior permission is to be taken from the journal office if any part of the published articles is to be reproduced in any form in any other publication.

How to Cite

M KAVYA, R DURGAPRASAD, VIBHAPANDEY, & V DINESHKUMAR. (2023). Genetic transformation of Trichoderma with GUS and Hyg Genes to study root colonisation in Groundnut: Genetic transformation of Trichoderma with GUS and Hyg Genes to study root colonisation in Groundnut. Journal of Oilseeds Research, 40(Specialissue). https://doi.org/10.56739/jor.v40iSpecialissue.145614