Efficient production of induced double haploid (DH) plants derived from anther culture: Efficient production of induced double haploid (DH) plants derived from anther culture

SHRINKHLA MAURYA; BHUMIKA SHARMA; SUMAN RAWTE; ZENU JHA

doi:10.56739/j77wm986

Authors

SHRINKHLA MAURYA Dept. of Plant Molecular Biology and Biotechnology, Indira Gandhi Krishi Vishwavidyalaya, Raipur, C.G 492 012, India
BHUMIKA SHARMA Dept. of Plant Molecular Biology and Biotechnology, Indira Gandhi Krishi Vishwavidyalaya, Raipur, C.G 492 012, India
SUMAN RAWTE Dept. of Plant Molecular Biology and Biotechnology, Indira Gandhi Krishi Vishwavidyalaya, Raipur, C.G 492 012, India
ZENU JHA Dept. of Plant Molecular Biology and Biotechnology, Indira Gandhi Krishi Vishwavidyalaya, Raipur, C.G 492 012, India

https://doi.org/10.56739/j77wm986

Keywords:

Niger,vegetable oil

Abstract

The success of DH technology dependents on the efficacy of optimized protocol that could be utilized for large scale production of homozygous lines. In this study, anther culture protocol was established using invitro colchicine treatment of haploid callus derived from anthers of niger (Guizotia abyssinica L.F. cass). Flower buds at uninucleate stage were kept for 24 hours cold pre-treatment followed by anther inoculation. 30-40 days old callus were subjected to different colchicine treatments (0, 0.025%, 0.05%, 0.1%, 0.2%) for different time intervals (6,16, 24, 30, 48hours). Colchicine treated (CT) callus were transferred to greening media followed by transferring to shooting media. Observations of greening and shooting percentage were taken with reference to control. The greening percentage found to be decreased (34%) compared to control (40%). However, 90-95% and 85-90% increment in shooting and rooting percentage was observed at 0.2% CT for 30 hours as compared to control which showed 15-20% shooting and 40-45% rooting. It was concluded that the incorporation of CT at callus stage increase the overall efficiency of DH production.

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References

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Efficient production of induced double haploid (DH) plants derived from anther culture