Geographic specificity of infestation of phytoplasma in weeds and sesame as revealed by 16S rRNA gene based detection

Geographic specificity of infestation of phytoplasma in weeds and sesame as revealed by 16S rRNA gene based detection


139 / 1

Authors

  • K B DURGA BHAVANI Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana
  • V DINESH KUMAR Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana
  • KT RAMYA Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana
  • LP SUMAN Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana

https://doi.org/10.56739/8zk3bh88

Keywords:

Nested PCR, Phyllody, 16SrRNA, Sequence analysis

Abstract

Nested PCR based study and analysis of the sequences of 16SrRNA fragment clones derived from the DNA isolated phyllody affected as well as healthy leaves of sesame crop and the weeds growing in the same fields showed that the prevalent strain of phytoplasma belonged to Candidatus Phytoplasma aurantifolia of 16SrII-D group. This study showed that the phytoplasma causing phyllody infects other weed plants and thus provides a hint to the continuum in the availability of the pathogen infecting sesame crop. 

Downloads

Download data is not yet available.

References

Bertaccini, A. (2022). Plants and Phytoplasmas: When Bacteria Modify Plants. Plants 11, 1425, 1-20 DOI: https://doi.org/10.3390/plants11111425

Deng, S. & Hiruki, C. (1991). Amplification of 16S rRNA genes from culturable and non-culturable mollicutes. J Microbiol Methods, 14, 53–61 DOI: https://doi.org/10.1016/0167-7012(91)90007-D

Gundersen, D. E. & Lee, I.-M. (1996). Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopathol Mediterr, 35, 144–151.

Liefting, L. W., Shaw, M. & Kirkpatrick, B. C. (2004). Sequence analysis of two plasmids from the phytoplasma beet leafhopper-transmitted virescence agent. Microbiology, 150, 1809–1817. DOI: https://doi.org/10.1099/mic.0.26806-0

Wei, W., Davis, R. E., Lee, I.-M. & Zhao, Y. (2007). Computersimulated RFLP analysis of 16S rRNA genes: identification of ten new phytoplasma groups. Int J Syst Evol Microbiol, 57, 1855–1867. DOI: https://doi.org/10.1099/ijs.0.65000-0

Journal of Oilseeds Research

Downloads

Submitted

2023-12-13

Published

2023-12-14

Issue

Vol. 40 No. Specialissue (2023): Journal of Oilseeds Research

Section

Articles

License

The Journal of Oilseeds Research reserves all the copy rights of the published articles and prior permission is to be taken from the journal office if any part of the published articles is to be reproduced in any form in any other publication.

How to Cite

K B DURGA BHAVANI, V DINESH KUMAR, KT RAMYA, & LP SUMAN. (2023). Geographic specificity of infestation of phytoplasma in weeds and sesame as revealed by 16S rRNA gene based detection: Geographic specificity of infestation of phytoplasma in weeds and sesame as revealed by 16S rRNA gene based detection. Journal of Oilseeds Research, 40(Specialissue). https://doi.org/10.56739/8zk3bh88