Molecular characterization of tobacco streak virus isolates causing sunflower necrosis disease in Southern India
MOLECULAR CHARACTERIZATION OF TSV ISOLATES CAUSING SUNFLOWER NECROSIS DISEASE
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Authors
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G V CHAITRA
Department of Plant Pathology, Agricultural College, Bapatla, Acharya N.G. Ranga Agricultural University, Guntur-522 034, Andhra Pradesha Pradesh
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B PARAMESWARI
Division of Plant Quarantine, ICAR-National Bureau of Plant Genetic Resources, Hyderabad-500030
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BHASKAR BAJARU
Division of Plant Quarantine, ICAR-National Bureau of Plant Genetic Resources, Hyderabad-500030
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L SARAVANAN
Division of Plant Quarantine, ICAR-National Bureau of Plant Genetic Resources, Hyderabad-500030
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D RAMESH
Department of Plant Pathology, Agricultural College, Bapatla, Acharya N.G. Ranga Agricultural University, Guntur-522 034, Andhra Pradesh
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V PRASANNA KUMARI
Department of Plant Pathology, Agricultural College, Bapatla, Acharya N.G. Ranga Agricultural University, Guntur-522 034, Andhra Pradesh
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V CELIA CHALAM
Division of Plant Quarantine, ICAR-National Bureau of Plant Genetic Resources, PUSA campus, New Delhi-110012
Keywords:
Genetic diversity, RNA virus, Tobacco streak virus, Sunflower necrosis diseaseAbstract
Sunflower necrosis disease (SND), caused by tobacco streak virus (TSV), is a major constraint to sunflower production in India. In this study, five TSV isolates collected from sunflower fields in Southern India were confirmed by RT-PCR and subjected to complete genome sequencing. All isolates exhibited the typical tripartite genome organization of Ilarvirus, encoding the replicase, RNA-dependent RNA polymerase, RNA silencing suppressor, movement protein, and coat protein. Conserved functional domains, including methyltransferase, helicase, and RdRp regions, were identified across all isolates. Comparative sequence and phylogenetic analyses showed that the sunflower isolates clustered closely with other Indian TSV isolates and were clearly separated from foreign isolates, indicating geographic structuring. Recombination analysis revealed multiple recombination events across all three genomic RNAs, suggesting that recombination contributes to TSV genetic diversity. This study enhances understanding of TSV genome diversity and evolution in sunflower-growing regions of Southern India.
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References
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