Establishment of GISH and multi-color GISH techniques to simultaneously discriminate different genomes in Erianthus procerus x Saccharum officinarum introgressed clones
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Authors
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Sobhakumari V.P
ICAR- Sugarcane Breeding Institute
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Mohanraj K
ICAR- SUGARCANE BREEDING INSTITUTE
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Mohanaprabha M
ICAR- SUGARCANE BREEDING INSTITUTE
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Mathumathi R
ICAR- SUGARCANE BREEDING INSTITUTE
Keywords:
Saccharum; Erianthus; Introgression; Mitosis; GISH; Multi-color GISHAbstract
An attempt was made to identify the chromosomes from different species genomes in BC₂ progenies of Erianthus procerus x Saccharum officinarum through multi-color GISH (MCGISH). The BC₂ population was derived from the cross between a BC1 hybrid (GU 12-14) and a commercial variety, Co 16018. The BC2 progenies were studied for their somatic chromosome number and two cytotypes were observed, i.e., 2n=92 (2) and 2n=96 (5). GISH revealed that the majority of progenies were having nine Erianthus chromosomes, except 2 clones (25-5 and 25-10) were having nine Erianthus chromosomes with one chromosome fragment. The methodology for multi-color GISH was standardized and it distinguished the chromosomes from different genomes. Interspecific recombination was also detected in somatic cells of one BC₂ clone, 25-10. The hybridity of the introgressed lines has been confirmed with amplification of Erianthus specific tandem repeat sequences.
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