Cloning of NAC 1 gene from Erianthus arundinaceus and development of transformation vector for sugarcane


Abstract views: 68 / PDF downloads: 50

Authors

  • Jini Narayanan ICAR- Sugarcane Breeding Institute , Crop Improvement Division, Coimbatore
  • Lakshmi Kasirajan ICAR- SBI, Senior Scientist, Biotechnology, Crop Improvement Division, Coimbatore
  • Ram Vannish ICAR- SBI, Crop Improvement Division, Coimbatore
  • Karthigeyan S Principal Scientist, Division of Crop Improvement, ICAR-SBI, Coimbatore
  • Selvi Athiappan Principal Scientist, Biotechnology, Division of Crop Improvement, ICAR-SBI, Coimbatore
  • Gomathi Raju Principal Scientist, Division of Crop Production, ICAR-SBI, Coimbatore
  • R. Manimekalai ICAR - SUGARCANE BREEDING INSTITUTE

https://doi.org/10.37580/JSR.2022.1.12.52-62

Keywords:

Saccharum spontaneum; Oxidative stress; Principal component analysis

Abstract

Genetic engineering approach is a viable method to impart abiotic stress tolerance in crop plants. The genomic compatability between Saccharum and related genera allows for broader application in sugarcane improvement programme in terms of improved hybrid productivity and adaptability. NAC tf (NAM ATAF CUC transcription factor) was found to be one of the most stress responsive transcription factors consisting of consortium of genes acting against both abiotic and biotic stress. Several studies have proved the involvement of NAC protein in imparting stress tolerance. Based on their response to abiotic stimuli, a set of NAC genes have been designated as Stress associated NAC (SNACs). We have sequenced NAC1 gene from Erianthus sp-IK76- 91 which is a stress tolerant species closely related to sugarcane. A 1.8 kb fragment was amplified in oxidative stress tolerant Erianthus using NAC1 specific primers. Gene construct was developed with binary vector pRI and the isolated gene was sequenced. BLASTn analysis of recombinant pRI 909 showed 88.61% homology with the Sorghum bicolor NAC1 gene. The oxidative stress responsive pRI::EaNAC construct developed can be further utilised to transform high yielding susceptible sugarcane varieties to benefit them in adapting to multiple abiotic stresses.

Author Biographies

  • Jini Narayanan, ICAR- Sugarcane Breeding Institute , Crop Improvement Division, Coimbatore

    PhD Scholar, ICAR- Sugarcane Breeding Institute , Crop Improvement Division, Coimbatore

  • R. Manimekalai, ICAR - SUGARCANE BREEDING INSTITUTE

    PRINCIPAL SCIENTIST, ICAR - SUGARCANE BREEDING INSTITUTE

References

Chohan M. 2019. Impact of climate change on sugarcane crop and remedial measures-a review. Pakistan Sugar Journal 34(1): 15–22.

Doyle JJ, Doyle JL.1990. Isolation of Plant DNA from Fresh Tissue. Focus 12: 13-15.

Hao YJ, Wei W, Song QX, Chen HW, Zhang YQ, Wang F, Chen SY. 2011. Soybean NAC transcription factors promote abiotic stress tolerance and lateral root formation in transgenic plants. The Plant Journal 68(2): 302-313.

Hu H, Dai M, Yao J, Xiao B, Li X, Zhang Q. 2006. Over-expressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice. Proceedings of the National Academy of Sciences, U.S.A. 103: 12987–12992

Liu QL, Xu KD, Zhao LJ, Pan YZ, Jiang BB, Zhang HQ, Liu GL.2011. Overexpression of a novel chrysanthemum NAC transcription factor gene enhances salt tolerance in tobacco. Biotechnology letters 33(10): 2073-2082.

Liu G, Li X, Jin S, Liu X, Zhu L, Nie Y, Zhang X. 2014. Overexpression of rice NAC gene SNAC1 improves drought and salt tolerance by enhancing root development and reducing transpiration rate in transgenic cotton. PLoS One 9(1): e86895.

Lu M, Ying S, Zhang DF, Shi YS, Song YC, Wang TY, Li Y.2012. A maize stress-responsive NAC transcription factor, ZmSNAC1, confers enhanced tolerance to dehydration in transgenic Arabidopsis. Plant cell reports 31(9): 1701-1711.

Mao X, Zhang H, Qian X, Li A, Zhao G, Jing R.2012. TaNAC2, a NAC-type wheat transcription factor conferring enhanced multiple abiotic stress tolerances in Arabidopsis. Journal of Experimental Botany 63(8): 2933-2946.

Manimekalai R, Jini Narayanan, Gokul M, Selvi A, Arun Meena, Gomathi R, Bakshi Ram, 2017. Genome wide analysis of NAC gene family 'sequences' in sugarcane and its comparative phylogenetic relationship with rice, sorghum, maize and Arabidopsis for prediction of stress associated NAC genes. Agrigene 3: 1–11. DOI: 10.1016/j.aggene. 2016. 10.003

Manimekalai R, Narayanan J, Ranjini R, Gokul M, Selvi A, Pradheep Kumar R. Gomathi R. 2018a. Hydrogen peroxide induced oxidative stress in sugarcane and response expression pattern of stress responsive gene through quantitative RTPCR. Sugar Tech. https://doi.org/10.1007/s12355-018-0604-4

Manimekalai R, Narayanan J, Gokul M, Selvi A, Gomathi R. 2018b. Biochemical and physiological response to oxidative stress in cultivated sugarcane and wild genera. Indian Journal of Plant Physiology 23(2): 261–270.

Manimekalai R, Selvi A, Narayanan J, Ram Vannish, Shalini, R, Gayathri S. Rabisha VP. 2023. Comparative physiological and transcriptome analysis in cultivated and wild sugarcane species in response to hydrogen peroxide-induced oxidative stress. BMC Genomics 24: 155. https://doi.org/10.1186/s12864-023-09218

Mayavan S, Subramanyam K, Arun M, Rajesh M, Kapil Dev G, Sivanandhan G, Jaganath B, Manickavasagam M, Selvaraj N, Ganapathi A.2013. Agrobacterium tumefaciens mediated in planta seed transformation strategy in sugarcane. Plant Cell Reports 32(10): 1557–1574.

Narayanan J, Manimekalai R, Selvi A,Arun Kumar R.2022. Physiological, biochemical and molecular responses to Oxidative stress in Saccharum spontaneum. Sugar Tech. 10.1007/s12355-022-01189-1.

Nuruzzaman M, Manimekalai R, Sharoni AM. (2010). Genome-wide analysis of NAC transcription factor family in rice. Gene 465: 30–40.

Puranik S, Sahu PP, Srivastava PS Prasad M. 2012. NAC proteins: regulation and role in stress tolerance. Trends in plant science 17(6):369-381.

Ramegowda V, Senthil-Kumar M, Nataraja KN, Reddy MK, Mysore KS, Udayakumar M. 2012. Expression of a finger millet transcription factor, EcNAC1, in tobacco confers abiotic stress-tolerance. PloS One 7(7): e40397.

Saravanan S, Kumar KK, Raveendran M, Sudhakar D, Arul L, Kokiladevi E. 2018. Genetic engineering of sugarcane for

drought and salt tolerant transgenic plants expressing the BcZAT12 gene. Journal Homepage: Http://Www. Ijcmas. Com,7(07).

Sambrook J, Fritsch EF, Maniatis T.1989. Molecular cloning: a laboratory manual. (Issue Ed. 2). Cold spring harbor laboratory press.

Sambrook J, Russell D.2006. Preparation and Transformation of Competent E. coli Using Calcium Chloride. CSH protocols. 2006. 10.1101/pdb.prot3932.

Song SY, Chen Y, Chen J, Dai XY, Zhang WH.2011. Physiological mechanisms underlying OsNAC5-dependent tolerance of rice plants to abiotic stress. Planta 234:331–345. doi: 10.1007/s00425-011-1403-2. pmid:21448719

Souer E, van Houwelingen A, Kloos D, Mol J, Koes R.1996. The No Apical Meristem gene of petunia is required for pattern formation in embryos and flowers and is expressed at meristem and primordia boundaries. Cell 85: 159–170

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30(12): 2725–2729.

Yilmaz A, Nishiyama Jr. MY, Fuentes BG, Souza GM, Janies D, Gray J, Grotewold E. 2009. GRASSIUS: a platform for comparative regulatory genomics across the grasses. Plant physiology 149(1): 171-180.

Zheng XN, Zhen B, Lu GJ, Han B. 2009. Overexpression of a NAC transcription factor enhances rice drought and salt tolerance. Biochemical and Biophysical Research Communications 379: 985–989. 10.1016/j.bbrc.2008.12.163

Downloads

Submitted

24-04-2023

Published

27-08-2023

How to Cite

Cloning of NAC 1 gene from Erianthus arundinaceus and development of transformation vector for sugarcane. (2023). Journal of Sugarcane Research, 12(1), 52-62. https://doi.org/10.37580/JSR.2022.1.12.52-62
Citation