Cloning of NAC 1 gene from Erianthus arundinaceus and development of transformation vector for sugarcane

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  • Jini Narayanan ICAR- Sugarcane Breeding Institute , Crop Improvement Division, Coimbatore
  • Lakshmi Kasirajan ICAR- SBI, Senior Scientist, Biotechnology, Crop Improvement Division, Coimbatore
  • Ram Vannish ICAR- SBI, Crop Improvement Division, Coimbatore
  • Karthigeyan S Principal Scientist, Division of Crop Improvement, ICAR-SBI, Coimbatore
  • Selvi Athiappan Principal Scientist, Biotechnology, Division of Crop Improvement, ICAR-SBI, Coimbatore
  • Gomathi Raju Principal Scientist, Division of Crop Production, ICAR-SBI, Coimbatore


Saccharum spontaneum; Oxidative stress; Principal component analysis


Genetic engineering approach is a viable method to impart abiotic stress tolerance in crop plants. The genomic compatability between Saccharum and related genera allows for broader application in sugarcane improvement programme in terms of improved hybrid productivity and adaptability. NAC tf (NAM ATAF CUC transcription factor) was found to be one of the most stress responsive transcription factors consisting of consortium of genes acting against both abiotic and biotic stress. Several studies have proved the involvement of NAC protein in imparting stress tolerance. Based on their response to abiotic stimuli, a set of NAC genes have been designated as Stress associated NAC (SNACs). We have sequenced NAC1 gene from Erianthus sp-IK76- 91 which is a stress tolerant species closely related to sugarcane. A 1.8 kb fragment was amplified in oxidative stress tolerant Erianthus using NAC1 specific primers. Gene construct was developed with binary vector pRI and the isolated gene was sequenced. BLASTn analysis of recombinant pRI 909 showed 88.61% homology with the Sorghum bicolor NAC1 gene. The oxidative stress responsive pRI::EaNAC construct developed can be further utilised to transform high yielding susceptible sugarcane varieties to benefit them in adapting to multiple abiotic stresses.

Author Biographies

  • Jini Narayanan, ICAR- Sugarcane Breeding Institute , Crop Improvement Division, Coimbatore

    PhD Scholar, ICAR- Sugarcane Breeding Institute , Crop Improvement Division, Coimbatore




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How to Cite

Cloning of NAC 1 gene from Erianthus arundinaceus and development of transformation vector for sugarcane. (2023). Journal of Sugarcane Research, 12(1), 52-62.