Cloning of NAC 1 gene from Erianthus arundinaceus and development of transformation vector for sugarcane

Jini Narayanan; Lakshmi Kasirajan; Ram Vannish; Karthigeyan S; Selvi Athiappan; Gomathi Raju; R. Manimekalai

doi:10.37580/JSR.2022.1.12.52-62

Authors

Jini Narayanan ICAR- Sugarcane Breeding Institute , Crop Improvement Division, Coimbatore
Lakshmi Kasirajan ICAR- SBI, Senior Scientist, Biotechnology, Crop Improvement Division, Coimbatore
Ram Vannish ICAR- SBI, Crop Improvement Division, Coimbatore
Karthigeyan S Principal Scientist, Division of Crop Improvement, ICAR-SBI, Coimbatore
Selvi Athiappan Principal Scientist, Biotechnology, Division of Crop Improvement, ICAR-SBI, Coimbatore
Gomathi Raju Principal Scientist, Division of Crop Production, ICAR-SBI, Coimbatore
R. Manimekalai ICAR - SUGARCANE BREEDING INSTITUTE

https://doi.org/10.37580/JSR.2022.1.12.52-62

Keywords:

Saccharum spontaneum; Oxidative stress; Principal component analysis

Abstract

Genetic engineering approach is a viable method to impart abiotic stress tolerance in crop plants. The genomic compatability between Saccharum and related genera allows for broader application in sugarcane improvement programme in terms of improved hybrid productivity and adaptability. NAC tf (NAM ATAF CUC transcription factor) was found to be one of the most stress responsive transcription factors consisting of consortium of genes acting against both abiotic and biotic stress. Several studies have proved the involvement of NAC protein in imparting stress tolerance. Based on their response to abiotic stimuli, a set of NAC genes have been designated as Stress associated NAC (SNACs). We have sequenced NAC1 gene from Erianthus sp-IK76- 91 which is a stress tolerant species closely related to sugarcane. A 1.8 kb fragment was ampliﬁed in oxidative stress tolerant Erianthus using NAC1 speciﬁc primers. Gene construct was developed with binary vector pRI and the isolated gene was sequenced. BLASTn analysis of recombinant pRI 909 showed 88.61% homology with the Sorghum bicolor NAC1 gene. The oxidative stress responsive pRI::EaNAC construct developed can be further utilised to transform high yielding susceptible sugarcane varieties to beneﬁt them in adapting to multiple abiotic stresses.

Author Biographies

Jini Narayanan, ICAR- Sugarcane Breeding Institute , Crop Improvement Division, Coimbatore

PhD Scholar, ICAR- Sugarcane Breeding Institute , Crop Improvement Division, Coimbatore
R. Manimekalai, ICAR - SUGARCANE BREEDING INSTITUTE

PRINCIPAL SCIENTIST, ICAR - SUGARCANE BREEDING INSTITUTE

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