In Vitro Studies on Effect of Endophytic Bacteria and Rhizobium on Seed Quality Parameters in Chickpea
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Authors
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HARISHKUMAR LAXMAN KANABUR
University of Agricultural Sciences, Dharwad, Karnataka-580005, India
Author
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ASHOK S SAJJAN
University of Agricultural Sciences, Dharwad, Karnataka-580005, India
Author
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GP SANTHOSH
University of Agricultural Sciences, Dharwad, Karnataka-580005, India
Author
Keywords:
Chickpea, Seed quality, EndophytesAbstract
Endophytes are microorganisms that reside in the tissues of living plants are relatively unstudied as
a potential source of novel natural products for exploitation in medicine, agriculture and industry. In present study,
the endophytes were extracted from aerial parts of chickpea (stem and leaves) brought from three different villages
of Vijayapur district. The isolation of bacteria was done and their morphological and biochemical properties were
studied. The screening was done to select efficient endophytes based on compatibility test between Rhizobium
GV-2 and the isolated endophytic bacteria and indole acetic acid test. Accordingly, three efficient endophytes BL4, MS-1 and MS-4 were selected and identified as Bacillus thuringiensis, B. cereus strain LPDB2 and B. cereus
strain LPDB5 based on 16S rRNA molecular identification. These three efficient endophytes along with the
Rhizobium GV-2 were treated with the chickpea seeds in different combinations. The investigation has aimed in
seed quality parameters under in vitro condition. Seed quality parameters include seed germination percentage,
seedling length, seedling vigour index-I and test weight. The significant difference was observed among the
treatments and the results revealed that treatment with B. thuringiensis + B. cereus strain LPDB2 + B. cereus
strain LPDB5 + Rhizobium GV-2 noticed significantly higher performance in seed quality parameters.
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