Influence of Seed Invigoration on Seed Quality in Upland Rice Genotypes


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Authors

  • VIDYA CJ University of Agricultural Sciences, Dharwad, Karnataka–580005, India Author
  • GURUMURTHY R University of Agricultural Sciences, Dharwad, Karnataka–580005, India Author
  • NK BIRADAR PATIL University of Agricultural Sciences, Dharwad, Karnataka–580005, India Author
  • HANAMARATTI NG University of Agricultural Sciences, Dharwad, Karnataka–580005, India Author

https://doi.org/10.56093/sr.v50i2.154562

Keywords:

Rice, gibberllic acid, ascorbic acid, azospirullum, priming

Abstract

A lab experiment was conducted to study the Influence of seed invigoration technique on seed quality in rice genotypes in the department of seed science and technology during 2020-21. Results revealed that among elite genotypes ‘BD 1’ and ‘BA 4’ found significantly superior in enzymatic activity and seed quality parameters. Among different treatments, the seed priming treatment with gibberllic acid @ 60 ppm for 12 hours recorded significantly higher values for enzymatic activity and seed quality parameters. Followed by seed treatment with ascorbic acid 10 ppm for 48 hours followed by seed treatment with azospirillum @ 1:50 dilution for 18 hours followed by seed treatment with distilled water for 48 hours and control. Among different treatments and genotypes combinations, seed priming treatment with gibberllic acid @ 60 ppm for 12 hours in genotype BD1 and BA4 registered significantly superior seed quality parameters. Decreased enzymatic activity viz., alpha amylase (1.98 cm to 0.38 cm), dehydrogenase (1.054 to 0.724 OD @ A480 nm), catalase (94.05 to 45.15 µmoles H2O2 decomposed/ min/g of seed fresh weight), peroxidase (5.91 to 2.78 µmoles/min/g of seed fresh weight) and SOD activity (22.27 to 12.59 Units/min/g seed fresh weight respectively) it is associated with decline in seed quality parameters viz., germination (88.50% to 77.5%), seedling shoot length (25.42cm to 13.3 cm), seedling root length (28.54 cm to 16.07 cm) and seedling dry weight (161.75 mg to 144.9 mg), field emergence (83.5% to 72.5%), seedling vigour
index I (2374 to 1138), seedling vigour index II (4332 to 2371).

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References

ANONYMOUS, 2013, www.indiastat.com

ANONYMOUS, 2019, http: www.ricepedia.org.

ANONYMOUS, 2019, www.indiastat.com.

AJOURI, A., ASGEDOM, H. AND BECKER, M., 2004. Seed

priming enhances germination and seedling growth of barley

under conditions of P and Zn deficiency. J. Plant Nutri. Soil

Sci., 167(5): 630-636.

MCDONALD, M.T., LADA, R.R., ROBINSON, A.R. AND

HOYLE, J., 2009, Seed preconditioning with natural and

synthetic antioxidants induces dr ought tolerance in tomato

seedlings. Hortsci., 44(5): 1323-1329.

AEBI, H., 1984, Catalase in vitro Methods in enzymology (105:

–126).

CASTILLO, M.D., STENSTROM, J. AND ANDER, P., 1994.

Determination of manganese peroxidase activity with 3-methyl2-benzothiazolinone hydrazone and 3-(dimethylamino)

benzoic acid. Anal, Biochem, 218(2): 399-404.

BEAUCHAMP, C. AND FRIDOVICH, I., 1971, Superoxide

dismutase: improved assays and an assay applicable to

acrylamide gels. Analyt. Biochem., 44(1): 276-287.

BELEIA, A., VARRIANO-MARSTON, E., 1981, Properties of

partially purified α-amylase. Cereal Chem. 58(5):433-437.

KITTOCK, D.L. AND LAW, A.G., 1968, Relationship of Seedling

Vigor to Respiration and Tetrazolium Chloride Reduction by

Germinating Wheat Seeds 1. Agron. J., 60(3): 286-288.

Simpson, H.E. and Naylor, R.M., 1962. Methods to detect

enzymes activity. Crop Sci., 40: S-26.

SOEDA, Y., KONINGS, M.C., VORST, O., VAN

HOUWELINGEN, A.M., STOOPEN, G.M., MALIEPAARD,

C.A., KODDE, J., BINO, R.J., GROOT, S. P. AND VAN DER

GEEST, A.H., 2005. Gene expression programs during

Brassica oleracea seed maturation, osmopriming, and

germination are indicators of progression of the germination

process and the stress tolerance level. Plant Physiol., 137(1):

-368.

COOLBEAR, P., SLATER, R. J. AND BRYANT, J. A., 1990,

Changes in nucleic acid levels associated with improved

germination performance of tomato seeds after low

temperature pre sowing treatment. Ann. Bot., 65(2): 187-195.

MONDAL, B.C., MUKHERJEE, T., MANDAL, L., EVANS, C.J.,

SINENKO, S.A., MARTINEZ-AGOSTO, J.A. AND BANERJEE,

U., 2011. Interaction between differentiating cell-and nichederived signals in hematopoietic progenitor maintenance. Cell,

(7): 1589-1600.

MAHAKHAM, W., SARMAH, A.K., MAENSIRI, S. AND

THEERAKULPISUT, P., 2017, Nanopriming technology for

enhancing germination and starch metabolism of aged rice

seeds using phytosynthesized silver nanoparticles. Scientific

Reports, 7(1): 1-21.

GIDROL, X., NOUBHANI, A., MOCQUOT, B., FOURNIER, A.

AND PRADET, A., 1998. Effect of accelerated aging on protein

synthesis in two legume seeds. Plant Physiology and

Biochemistry (Paris), 26(3): 281-288.

KAPOOR, N., ARYA, A., SIDDIQUI, M.A., AMIR, A. AND

KUMAR, H., 2010. Seed deterioration in chickpea (Cicer

arietinum L.) under accelerated ageing. Asian J. Plant Sci.,

(3): 158-162.

SOMASUNDARAM, G. AND BHASKARAN, M., 2017,

Standardization of Accelerated Ageing Duration for Screening

of Rice Genotypes for Seed Longevity. Australian J. Basic and

Applied Sci., 3(3): 87-93.

GOVINDARAJ, M., MASILAMANI, P., SELVARAJU, P. AND

ALBERT, V. A., 2017, Effect of accelerated ageing on

germination and seedling vigour of manually and mechanically

harvested and threshed rice seeds. Int. J. Agric. Sci. Res,

(4): 39-48.

HELA, M., HANEN, Z., IMEN, T., OLFA, B., NAWEL, N.,

RAOUIA, B.M., MAHA, Z., JUN, H., ABDELALI, H., MOKHTAR,

L. AND ZEINEB, O., 2012, Combined effect of hormonal

priming and salt treatments on germination percentage and

antioxidant activities in lettuce seedlings. African J. Biotechnol.,

(45): 10373-10380.

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Submitted

2024-08-02

Published

2026-03-27

How to Cite

VIDYA CJ, GURUMURTHY R, NK BIRADAR PATIL, & HANAMARATTI NG. (2026). Influence of Seed Invigoration on Seed Quality in Upland Rice Genotypes. Seed Research, 50(2), 110-117. https://doi.org/10.56093/sr.v50i2.154562