Effect of ROS Inhibitors and Scavengers on Seed Quality Parameters of Indian Mustard
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Authors
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NIPA BISWAS
ICAR- Indian Agricultural Research Institute, New Delhi -110012, India
Author
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SANGITA YADAV
ICAR- Indian Agricultural Research Institute, New Delhi -110012, India
Author
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SHIV K. YADAV
ICAR- Indian Agricultural Research Institute, New Delhi -110012, India
Author
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NAVINDER SAINI
ICAR- Indian Agricultural Research Institute, New Delhi -110012, India
Author
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PK MANDAL
ICAR- Indian Agricultural Research Institute, New Delhi -110012, India
Author
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ANIL DAHUJA
ICAR- Indian Agricultural Research Institute, New Delhi -110012, India
Author
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SUJATA VASUDEV
ICAR- Indian Agricultural Research Institute, New Delhi -110012, India
Author
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DK YADAVA
ICAR- Indian Agricultural Research Institute, New Delhi -110012, India
Author
Keywords:
Germination, Indian mustard, ROS, Seed vigour indicesAbstract
The present study was conducted to evaluate the effect of different ROS inhibitor and scavenger on the seed quality parameters of Indian mustard. Different concentrations of DPI, ZnCl2, CuCl2 and KI were tested. The concentrations which reduced the seed vigour index-I (SVI-I) by 50% of the normal were selected for further comparison with control. 0.01mM DPI, 20mM ZnCl2, 0.5mM DPI and 5mM KI concentrations were selected and compared with the control (distilled water). The results showed that there was no effect on physiological seed germination at these concentrations but other parameters of seed quality were reduced significantly. The reduction in root growth was more than the shoot growth and such reduction was significantly higher in DPI and CuCl2 treatment. Reduction in shoot and root length resulted in reduction in total seedling length and hence the SVI-I got reduced. At the concentrations where SVI-I got reduced by 50%, the reduction in seed vigour index-II was not much significant. Minimum reduction in SVI-II and dry weight was observed in KI treatment which may be associated with scavenging of hydrogen peroxide (H2O2), since H2O2 stimulate reserve mobilization process and KI is a scavenger of H2O2. So it can be concluded that inhibition in ROS production or scavenging of ROS during germination affect the seed quality parameters and restrict the growth and development of seedling. Such inhibitory effect is more on root growth than the shoot growth and SVI-I get more hampered than SVI-II.
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