Seed Priming: Technique, Applications and Future Prospects in Vegetable Crops
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Authors
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RAJINDER SINGH
Punjab Agricultural University, Ludhiana, Punjab-141004, India
Author
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VARINDA
Punjab Agricultural University, Ludhiana, Punjab-141004, India
Author
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LAVANYA VIJ
Punjab Agricultural University, Ludhiana, Punjab-141004, India
Author
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NAVJYOT KAUR
Punjab Agricultural University, Ludhiana, Punjab-141004, India
Author
Keywords:
DNA repair, Germination, Priming, Seed quality, Stress resistance, Vegetable cropsAbstract
Horticulturists all over the world are struggling to meet the market’s increasing demands for highyielding quality vegetable crops. Seed priming presents a sustainable strategy to improve crop’s physiological and field characteristics helping farmers and seed producers to ensure high crop productivity and minimise any additional input cost by ensuring optimum crop stand under normal as well as stress conditions. Current scientific trends suggest that priming may aid in the reversal of age and storage-induced seed deterioration. It aids in lowering the levels of reactive oxygen and reactive nitrogen species in seeds with better reserve mobilization, increased efficiency of antioxidant enzymes and DNA repair mechanisms. This review paper aims to summarise the latest trends in the seed priming works on the types, mechanism and efficacy of seed priming techniques in vegetable crops with a particular focus on its potential to recover aged seed via priming, along with highlighting the gaps in the current investigations that ought to be filled with future studies. The findings elucidate the great potential of priming as a technique to become a major part of integrative resource management and crop improvement programmes to address issues such as food security and crop productivity in the era of climate change.
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References
ANONYMOUS (2022). Vegetable seed market size, share and
COVID-19 impact analysis by type (open pollinated varieties
and hybrid), crop type (Solanaceae, root and bulb, cucurbit,
brassica, leafy and others), cultivation method (protected and
open field), regional forecast, 2021-2028. https://
www.fortunebusinessinsights.com/vegetable-seed-market103066
CHORMULE SR, CHANGADE NM AND PATEL JB (2018).
Effect of seed treatments on storability of vegetable seeds: a
review. Plant Archives, 18(1): 28-32.
MEMON N, GANDAHI MB, PAHOJA VM AND NASIM S
(2013). Response of seed priming with boron on germination
and seedling sprouts of broccoli. International Journal of
Agricultural Science and Research, 3(2): 183-194.
MCDONALD MB (1999). Seed deterioration: physiology, repair
and assessment. Seed Science and Technology, 27(1): 177-
FINCH-SAVAGE WE AND BASSEL GW (2016). Seed vigour
and crop establishment: extending performance beyond
adaptation. Journal of Experimental Botany, 67(3): 567-591.
SUDISHA J, NIRANJAN-RAJ S AND SHEKAR SHETTY H
(2009). Seed priming with plant gum biopolymers enhances
efficacy of metalaxyl 35 SD against pearl millet downy
mildew. Phytoparasitica, 37(2): 161-169.
MAL D, VERMA J, LEVAN A, REDDY MR, AVINASH AV AND
VELAGA PK (2019). Seed priming in vegetable crops: A
review. International Journal of Current Microbiology and
Applied Sciences, 8(6): 868-874.
BROCKLEHURST PA AND DEARMAN J (1983). Interactions
between seed priming treatments and nine seed lots of carrot,
celery and onion. II Seedling emergence and plant
growth. Annals of Applied Biology, 102(3): 585-593.
HUSSAIN S, ZHENG M, KHAN F, KHALIQ A, FAHAD S AND
PENG S (2015). Benefits of rice seed priming are offset
permanently by prolonged storage and the storage
conditions. Scientific Reports, 5(1): 1-12.
ULLAH A, FAROOQ M, HUSSAIN M, AHMAD R AND
WAKEEL A (2019). Zinc seed priming improves stand
establishment, tissue zinc concentration and early seedling
growth of chickpea. The Journal of Animal and Plant
Sciences, 29(4): 1046-1053.
VENKATASUBRAMANIAN A AND UMARANI R (2007).
Evaluation of seed priming methods to improve seed
performance of tomato (Lycoperison esculentum), egg plant
(Solanum melongena) and chilli (Capsicum annum). Seed
Science and Technology, 35(2): 487-493.
RAJ AB AND RAJ SK (2019). Seed priming: An approach
towards agricultural sustainability. Journal of Applied and
Natural Science, 11(1): 227-234.
MAHAWAR MK, SAMUEL DVK, SINHA JP AND
JALGAONKAR K (2016). Optimization of pea (Pisum sativum)
seeds hydropriming by application of response surface
methodology. Acta Physiologiae Plantarum, 38(9): 1-13.
ADHIKARI B, DHITAL PR, RANABHAT S AND POUDEL H
(2021). Effect of seed hydro-priming durations on germination
and seedling growth of bitter gourd (Momordica
charantia). PloS One, 16(8): e0255258.
DAMALAS CA, KOUTROUBAS SD AND FOTIADIS S (2019).
Hydro-priming effects on seed germination and field
performance of faba bean in spring sowing. Agriculture, 9(9):
SADEGHIAN SY AND YAVARI N (2004). Effect of water deficit
stress on germination and early seedling growth in sugar
beet. Journal of Agronomy and Crop Science, 190(2): 138-
CASENAVE EC AND TOSELLI ME (2010). Germination of
melon seeds under water and heat stress: hydropriming and
the hydrotime model. Seed Science and Technology, 38(2):
-420.
NOMKHOSI B, MABUZA M AND TANA T (2021). Effects of
osmo-priming on germination, growth and green pod yield of
okra (Abelmoschus esculentus (L.) Moench) at Luyengo,
Middleveld of Eswatini. World Journal of Advanced Research
and Reviews, 11(01): 029–038.
KIRAN KR, DEEPIKA VB, SWATHY PS, PRASAD K,
KABEKKODU SP, MURALI TS AND MUTHUSAMY A (2020).
ROS-dependent DNA damage and repair during germination
of NaCl primed seeds. Journal of Photochemistry and
Photobiology B: Biology, 213: 112050.
SINGH J, KANWAR JS AND GEETA B (2004). Seed vigour
as influenced by different seed priming treatments in okra
(Abelmoschus esculentus L. Moench). Seed Research, 32:
–125.
NALINI T, POONAM S, LAL C, KATIYAR PK AND VAISH CP
(2001). Effect of presowing seed treatments on germination
growth and yield of onion. Seed Research, 29: 238–39.
KUMAR S (2005) Influence of pre-sowing seed treatment and
seed pelleting on storability in brinjal (Solanum melongena
L). MSc (Agri) Thesis, University of Agriculture Science,
Dharwad, India.
YOGANANDA DK, VYAKARNAHAL BS AND
SHEKHARGOUDA M (2004). Effect of seed invigoration with
growth regulations and micronutrients on germination and
seedling vigour of bell pepper cv. California Wonder. Karnataka
Journal of Agricultural Science, 17(4): 811–13.
DA SILVA CB, MARCOS-FILHO J, JOURDAN P AND
BENNETT MA (2015). Performance of bell pepper seeds in
response to drum priming with addition of 24-
epibrassinolide. HortScience, 50(6): 873-878.
SUKANYA V, PATEL RM, SUTHAR KP AND SINGH D (2018).
An overview: mechanism involved in bio-priming mediated
plant growth promotion. International Journal of Pure and
Applied Bioscience, 6(5): 771-783.
SARKAR D, RAKSHIT A, PAREWA HP, DANISH S,
ALFARRAJ S AND DATTA R (2022). Bio-priming with
compatible rhizospheric microbes enhances growth and
micronutrient uptake of red cabbage. Land, 11(4): 536.
KAYMAK HÇ, GÜVENÇ Ý, YARALI F AND DÖNMEZ MF
(2009). The effects of bio-priming with PGPR on germination
of radish (Raphanus sativus L.) seeds under saline
conditions. Turkish Journal of Agriculture and Forestry, 33(2):
-179.
PAWAR VA AND LAWARE SL (2018). Seed priming a critical
review. International Journal of Advanced Research in
Biological Sciences, 5: 94-101.
LUTTS S, BENINCASA P, WOJTYLA L, KUBALA S, PACE R,
LECHOWSKA K AND GARNCZARSKA M (2016). Seed
priming: new comprehensive approaches for an old empirical
technique. New Challenges in Seed Biology-Basic and
Translational Research Driving Seed Technology, 1-46.
SINGH PK, PANDITA VK, TOMAR BS AND SETH R (2015).
Standardization of priming treatments for enhancement of seed
germination and field emergence in carrot. Indian Journal of
Horticulture, 72: 306-309.
KÊPCZYÑSKA E, PIÊKNA-GROCHALA J AND KÊPCZYÑSKI
J (2003). Effects of matriconditioning on onion seed
germination, seedling emergence and associated physical and
metabolic events. Plant Growth Regulation, 41(3): 269-278.
SANO N AND SEO M (2019). Cell cycle inhibitors improve
seed storability after priming treatments. Journal of Plant
Research, 132(2): 263-271.
ACHARYA P, JAYAPRAKASHA GK, CROSBY KM, JIFON JL
AND PATIL BS (2019). Green-synthesized nanoparticles
enhanced seedling growth, yield, and quality of onion (Allium
cepa L.). ACS Sustainable Chemistry and Engineering, 7(17):
-14590.
ACHARYA P, JAYAPRAKASHA GK, CROSBY KM, JIFON JL
AND PATIL BS (2020). Nanoparticle-mediated seed priming
improves germination, growth, yield, and quality of
watermelons (Citrullus lanatus) at multi-locations in
Texas. Scientific Reports, 10(1): 1-16.
BABY SM, NARAYANASWAMY GK AND ANAND A (2011).
Superoxide radical production and performance index of
Photosystem II in leaves from magnetoprimed soybean
seeds. Plant Signaling and Behavior, 6(11): 1635-1637.
RATHOD GR AND ANAND A (2016). Effect of seed magnetopriming on growth, yield and Na/K ratio in wheat (Triticum
aestivum L.) under salt stress. Indian Journal of Plant
Physiology, 21(1): 15-22.
GUPTA MK, ANAND A, PAUL V, DAHUJA A AND SINGH AK
(2015). Reactive oxygen species mediated improvement in
vigour of static and pulsed magneto-primed cherry tomato
seeds. Indian Journal of Plant Physiology, 20(3): 197-204.
BHARDWAJ J, ANAND A AND NAGARAJAN S (2012).
Biochemical and biophysical changes associated with
magnetopriming in germinating cucumber seeds. Plant
Physiology and Biochemistry, 57: 67-73.
HAMID N AND JAWAID F (2011). Influence of seed pretreatment by UV-A and UV-C radiation on germination and
growth of mung beans. Pakistan Journal of Chemistry, 1(4):
-167.
GUAJARDO FLORES D, SERNA GUERRERO D, SERNA
SALDÍVAR SO AND JACOBO VELÁZQUEZ DA (2014). Effect
of germination and UV C radiation on the accumulation of
flavonoids and saponins in black bean seed coats. Cereal
Chemistry, 91(3): 276-279.
OUHIBI C, ATTIA H, REBAH F, MSILINI N, CHEBBI M,
AARROUF J AND LACHAAL M (2014). Salt stress mitigation
by seed priming with UV-C in lettuce plants: Growth,
antioxidant activity and phenolic compounds. Plant Physiology
and Biochemistry, 83: 126-133.
KOTWALIWALE N, SINGH K, KALNE A, JHA SN, SETH N
AND KAR A (2014). X-ray imaging methods for internal quality
evaluation of agricultural produce. Journal of Food Science
and Technology, 51(1): 1-15.
DE MICCO V, PARADISO R, ARONNE G, DE PASCALE S,
QUARTO M AND ARENA, C (2014). Leaf anatomy and
photochemical behaviour of Solanum lycopersicum L. plants
from seeds irradiated with low-LET ionising radiation. The
Scientific World Journal, 2014.
VARIER A, VARI AK AND DADLANI M (2010). The subcellular
basis of seed priming. Current Science, 1: 450-456.
DALIL B (2014). Response of medicinal plants to seed priming:
a review. International Journal of Plant, Animal and
Environmental Sciences, 4(2): 741-745.
KAMITHI KD, WACHIRA F AND KIBE AM (2016). Effects of
different priming methods and priming durations on enzyme
activities in germinating chickpea (Cicer arietinum
L.). American Journal of Natural and Applied Sciences, 1(1):
-9.
PAPARELLA S, ARAÚJO SS, ROSSI G, WIJAYASINGHE M,
CARBONERA D AND BALESTRAZZI A (2015). Seed priming:
state of the art and new perspectives. Plant Cell Reports, 34(8):
-1293.
HUSSAIN A, RIZWAN M, ALI Q AND ALI S (2019). Seed
priming with silicon nanoparticles improved the biomass and
yield while reduced the oxidative stress and cadmium
concentration in wheat grains. Environmental Science and
Pollution Research, 26(8): 7579-7588.
GALHAUT L, DE LESPINAY A, WALKER DJ, BERNAL MP,
CORREAL E AND LUTTS S (2014). Seed priming of Trifolium
repens L. improved germination and early seedling growth on
heavy metal-contaminated soil. Water, Air and Soil
Pollution, 225(4): 1-15.
CHEN K, FESSEHAIE A AND ARORA R (2013). Aquaporin
expression during seed osmopriming and post-priming
germination in spinach. Biologia Plantarum, 57(1): 193-198.
WOJTYLA £, LECHOWSKA K, KUBALA S AND
GARNCZARSKA M (2016). Different modes of hydrogen
peroxide action during seed germination. Frontiers in Plant
Science, 7: 66.
PANT B AND BOSE B (2016). Mitigation of the influence of
PEG-6000 imposed water stress on germination of halo primed
rice seeds. International Journal of Agriculture, Environment
and Biotechnology, 9(2): 275.
DI GIROLAMO G AND BARBANTI L (2012). Treatment
conditions and biochemical processes influencing seed
priming effectiveness. Italian Journal of Agronomy, 7(2): e25.
PUKACKA S AND RATAJCZAK E (2005). Production and
scavenging of reactive oxygen species in Fagus sylvatica
seeds during storage at varied temperature and
humidity. Journal of Plant Physiology, 162(8): 873-885.
DAWOOD MG (2018). Stimulating plant tolerance against
abiotic stress through seed priming. In Advances in seed
priming (pp. 147-183). Springer, Singapore.
ÖZBINGÖL N, CORBINEAU F, GROOT SPC, BINO RJ AND
CÔME D (1999). Activation of the cell cycle in tomato
(Lycopersicon esculentum Mill.) seeds during
osmoconditioning as related to temperature and
oxygen. Annals of Botany, 84(2): 245-251.
CHEN K, FESSEHAIE A AND ARORA R (2012). Dehydrin
metabolism is altered during seed osmopriming and
subsequent germination under chilling and desiccation in
Spinacia oleracea L. cv. Bloomsdale: possible role in stress
tolerance. Plant Science, 183: 27-36.
YADAV PV, MAYA K AND ZAKWAN A (2011). Seed priming
mediated germination improvement and tolerance to
subsequent exposure to cold and salt stress in
capsicum. Research Journal of Seed Science, 4(3): 125-136.
WU L, HUO W, YAO D AND LI M (2019). Effects of solid matrix
priming (SMP) and salt stress on broccoli and cauliflower seed
germination and early seedling growth. Scientia
Horticulturae, 255: 161-168.
NAVITHA P, SUJATA K AND BEAULAH A (2019). Effect of
chemoprimimg on physiological quality of cucumber (Cucumis
sativus). International Journal of Chemical Studies, 7(2): 1729-
AMOOAGHAIE R, NIKZAD K AND SHAREGHI B (2010). The
effect of priming on emergence and biochemical changes of
tomato seeds under suboptimal temperatures. Seed Science
and Technology, 38(2): 508-512.
ANESE S, DA SILVA EAA, DAVIDE AC, ROCHA FARIA JM,
SOARES GCM, MATOS ACB AND TOOROP PE (2011). Seed
priming improves endosperm weakening, germination, and
subsequent seedling development of Solanum lycocarpum St.
Hill. Seed Science and Technology, 39(1): 125-139.
PRADHAN N, PRAKASH P, MANIMURUGAN C, TIWARI SK,
SHARMA RP AND SINGH PM (2015). Screening of tomato
genotypes using osmopriming with PEG 6000 under salinity
stress. Research in Environmental and Life Sciences, 8: 245-
SAINI R, RAI PK, BARA BM, SAHU P, ANJER T AND KUMAR
R (2017). Effect of different seed priming treatments and its
duration on seedling characters of bitter gourd (Momordica
charantia L.). Journal of Pharmacognosy and Phytochemistry,
(5): 848-850.
REHMAN H, FAROOQ M, BASRA SMA AND AFZAL I (2011).
Hormonal priming with salicylic acid improves the emergence
and early seedling growth in cucumber. Journal of Agricultural
Sciences, 7: 109-113.
SINGH DK AND PANDEY UB (2003). Effect of hydrationdehydration and priming on seed germination and vigour of
onion (Allium cepa L. var. Agrifound Dark Red) in field
conditions. NHRDF Newsletter, 23: 13-15.
RAJ AB, RAJ SK, PRATHAPAN K AND RADHAKRISHNAN
NV (2020). Nutripriming with zinc sulphate and borax for early
growth and seedling vigour in grain cowpea [Vigna unguiculata
(L.) Walp]. Legume Research-An International Journal, 43(2):
-262.
GANDAHI MB, NOOR-UN-NISA M, MEHRUNISA M, MIANO
TF AND ABBASI FF (2017). Effects of nutripriming on
germination and seedling growth of cole vegetables.
Bangladesh Journal of Botany, 46(2): 653-658.
SARKAR D AND RAKSHIT A (2021). Bio-priming in
combination with mineral fertilizer improves nutritional quality
and yield of red cabbage under Middle Gangetic Plains,
India. Scientia Horticulturae, 283: 110075
PATRA DD (2016). Unravelling plant-rhizopsphere-microbe
interactions: an overview. Journal of the Indian Society of Soil
Science, 64: 14-26.
SIVAKALAI R AND KRISHNAVENI K (2017). Effect of
biopriming on seed yield and quality in pumpkin cv CO
International Journal of Current Microbilogy and Applied
Sciences, 6(12): 85-90.
ANANTHI M, SELVARAJU P AND SUNDARALINGAM K
(2014). Effect of bio-priming using bio-control agents on seed
germination and seedling vigour in chilli (Capsicum annuum
L.) ‘PKM 1’. The Journal of Horticultural Science and
Biotechnology, 89(5): 564-568.
KANWAR R AND MEHTA DK (2017). Studies on solid matrix
priming of seeds in bitter gourd (Momordica charantia
L.). Journal of Applied and Natural Science, 9(1): 395-401.
ALMUTAIRI ZM AND ALHARBI A (2015). Effect of silver
nanoparticles on seed germination of crop plants. International
Journal of Agricultural and Biological Engineering, 9: 667-71.
DEHKOURDI EH, CHEHRAZI M, HOSSEINI H AND
HOSSEINI M (2014). The effect of anatase nanoparticles
(TiO2) on pepper seed germination (Capsicum annum
L.). International Journal of Biosciences, 4(5): 141-145.
HEGAZI AZ AND HAMIDELDIN N (2010). The effect of gamma
irradiation on enhancement of growth and seed yield of okra
[Abelmoschus esculentus (L.) Monech] and associated
molecular changes. Journal of Horticulture and Forestry, 2(3):
-051.
MOUSSA HR (2006). Role of gamma irradiation in regulation
of NO3 level in rocket (Eruca vesicaria subsp. sativa)
plants. Russian Journal of Plant Physiology, 53(2): 193-197.
PEREIRA MD, DIAS DCFDS, DIAS LADS AND ARAÚJO EF
(2009). Primed carrot seeds performance under water and
temperature stress. Scientia Agricola, 66: 174-179.
ANDREOTTI C (2020). Management of abiotic stress in
horticultural crops: spotlight on biostimulants. Agronomy,
(10):1514.
YAN M (2015). Seed priming stimulate germination and early
seedling growth of Chinese cabbage under drought
stress. South African Journal of Botany, 99: 88-92.
YAN M (2016). Hydro-priming increases seed germination and
early seedling growth in two cultivars of Napa cabbage
(Brassica rapa subsp. pekinensis) grown under salt stress. The
Journal of Horticultural Science and Biotechnology, 91(4): 421-
KHAN HA, AYUB, CM, PERVEZ MA, BILAL RM, SHAHID MA
AND ZIAF K (2009). Effect of seed priming with NaCI on salinity
tolerance of hot pepper (Capsicum annuum L.) at seedling
stage. Soil and Environment, 28(1): 81-87.
SARWAR M, AMJAD M AND AYYUB CM (2017). Alleviation
of salt stress in cucumber (Cucumis sativus) through seed
priming with triacontanol. International Journal of Agriculture
and Biology, 19:4.
GHASEEMI-GOLEZANI K AND ESMAEILPOUR B (2008).
The effect of salt priming on the performance of differentially
matured cucumber (Cucumis sativus) seeds. Notulae
Botanicae Horti Agrobotanici Cluj-Napoca, 36(2): 67-70.
WORRALL D, HOLROYD GH, MOORE JP, GLOWACZ M,
CROFT P, TAYLOR, JE AND ROBERTS MR (2012). Treating
seeds with activators of plant defence generates long lasting
priming of resistance to pests and pathogens. New
Phytologist, 193(3): 770-778.
SONG Y, CHEN D, LU K, SUN Z AND ZENG R (2015).
Enhanced tomato disease resistance primed by arbuscular
mycorrhizal fungus. Frontiers in Plant Science, 6, 786.
MAHESH HM, MURALI M, PAL MAC, MELVIN P AND
SHARADA MS (2017). Salicylic acid seed priming instigates
defense mechanism by inducing PR-Proteins in Solanum
melongena L. upon infection with Verticillium dahliae
Kleb. Plant Physiology and Biochemistry, 117: 12-23.
ENTESARI M, SHARIFZADEH F, AHMADZADEH M AND
FARHANGFAR M (2013). Seed biopriming with Trichoderma
species and Pseudomonas fluorescent on growth parameters,
enzymes activity and nutritional status of soybean.
International Journal of Agronomy and Plant Production, 4(4):
-619.
PILL WG, COLLINS CM, GOLDBERGER B AND GREGORY
N (2009). Responses of non-primed or primed seeds of
‘Marketmore 76’ cucumber (Cucumis sativus L.) slurry coated
with Trichoderma species to planting in growth media infested
with Pythium aphanidermatum. Scientia Horticulturae, 121(1):
-62.
TAYLOR AG, HARMAN GE AND NIELSEN PA (1994).
Biological seed treatments using Trichoderma harzianum for
horticultural crops. HortTechnology, 4(2): 105-109.
BROWN JE, LU TY, STEVENS C, KHAN VA, LU JY, WILSON
CL AND DROBY S (2001). The effect of low dose ultraviolet
light-C seed treatment on induced resistance in cabbage to
black rot (Xanthomonas campestris pv. campestris). Crop
Protection, 20(10): 873-883.
SONG GC, CHOI HK, KIM YS, CHOI JS AND RYU CM (2017).
Seed defense biopriming with bacterial cyclodipeptides triggers
immunity in cucumber and pepper Scientific Reports, 7(1), 1-
SINGH P, SINGH J, RAY S, RAJPUT RS, VAISHNAV A,
SINGH RK AND SINGH HB (2020). Seed biopriming with
antagonistic microbes and ascorbic acid induce resistance in
tomato against Fusarium wilt. Microbiological Research, 237:
AKELLO J, CHABI-OLAYE A AND SIKORA RA (2017). Insect
antagonistic bio-inoculants for natural control of leaf-mining
insect pests of french beans. African Crop Science
Journal, 25(2): 237-251.
RAJ AK, DAS H AND BASU AK (2019). Response of biopriming in okra for vegetable production. Journal of Applied
and Natural Sciences, 11: 687-693.
AGRAWAL RL (ed) (1995). Seed Technology. pp 410-12.
Oxford and IBH Publishing, New Delhi
KIBINZA S, BAZIN J, BAILLY C, FARRANT JM, CORBINEAU
F AND EL-MAAROUF-BOUTEAU H (2011). Catalase is a key
enzyme in seed recovery from ageing during priming. Plant
Science, 181(3): 309-315.
KIBINZA S, VINEL D, CÔME D, BAILLY C AND CORBINEAU
F (2006). Sunflower seed deterioration as related to moisture
