Characterization of Contrasting Soybean Genotypes for Seed Longevity Using SSR Markers
45 / 6
Authors
-
BHARAMARAJ BADIGER
UAS, Dharwad 580 005, Karnataka ICAR-Indian Institute of Millet Research, Rajendranagar, Hyderabad
Author
-
RAVI HUNJE
UAS, Dharwad 580 005, Karnataka ICAR-Indian Institute of Millet Research, Rajendranagar, Hyderabad
Author
-
DP BIRADAR
UAS, Dharwad 580 005, Karnataka ICAR-Indian Institute of Millet Research, Rajendranagar, Hyderabad
Author
-
NK BIRADAR PATIL
UAS, Dharwad 580 005, Karnataka ICAR-Indian Institute of Millet Research, Rajendranagar, Hyderabad
Author
-
VILAS TONAPI
UAS, Dharwad 580 005, Karnataka ICAR-Indian Institute of Millet Research, Rajendranagar, Hyderabad
Author
Keywords:
Soybean, Seed longevity, Genotype, PrimerAbstract
In Soybean [Glycine max (L.) Merrill], seed storability is a major problem and identifying the genotypes with good storability as well as the DNA marker associated with seed storability will help in Markers Assisted Selection (MAS) programme targeted for breeding soybean genotype with better seed storability. In this study, six genotypes of soybean, varying in storability, were characterized using 14 SSR markers that were reported to be linked or associated with seed longevity. Out of 14 primers screened, the primers Satt 285, Satt 316, Satt 434 and Satt 538 gave distinct banding pattern among the good and poor storers. They produced the allele of size range from 184 bp in genotypes having poor seed longevity and the allele of size 235 bp for those genotypes having good seed longevity.
Downloads
References
1. KARMARKAR PG, PS BHATNAGAR AND SM HUSAIN (1998). Identification of bold seeded soybean genotypes with good longevity in segregation generation of crosses. Soybean Genet Newslett 25: 76
2. CREGAN PB, T JARVIK, AL BUSH, RC SHOEMAKER, LARKK G, AL KAYA., TT VAN- TOAI, DG LOHNES, J CHUNG AND J ESPECHT (1999). An integrated genetic linkage map of the soybean genome. Crop Sci 39: 1464-1490
3. SINGH RK, RK RAIPURIA, VS BHATIA, R ANITA, PUSHPENDRA AND SM HUSAIN (2008). Identification of SSR markers associated with seed coat permeability and electrolyte leaching in soybean. Physiol Mol Biol Pl 14(3): 173-177
4. KHARE D, SAHU P, SAINI N, AN SHRIVASTAVA AND MS BHALE (2011). Soybean seed longevity. Paper presented in National Seed Congress pp: 217- 224
5. SAGHAI-MAROOF MA, KM SOLIMAN, RA JORGENSEN AND RW ALLARD (1984). Ribosomal DNA spacer length polymorphism in barley. Mendelian inheritance, chromosomal location and population dynamics. Proc Nat Academy Sci USA 81: 8014-8018
6. JAGADISH H, MB ARUN KUMAR, TALUKDAR A, SK LAL AND M DADLANI (2013). Molecular characterization and identification of candidate markers for seed longevity in soybean [Glycine max (L.) Merrill]. Indian J Genet 73(1): 64-71
7. SHILPA MN (2012). Genetic variability for seed longevity and component traits in soybean [Glycine max (L.) Merrill]. M Sc (Agri.) Thesis Univ Agric Sci Dharwad, Karnataka (India)
8. ROHLF FJ (1998). NTSYS-PC Numerical Taxonomy and Multivariate Analysis, ver. 2. 0. Applied Biostatics Inc New York pp: 37.
