Characterization of seed longevity traits by using molecular markers in soybean [Glycine max (L.) Merrill]
51 / 3
Authors
-
BHARAMARAJ BADIGER
University of Agricultural Sciences, Dharwad-580 005
Author
-
RAVI HUNJE
University of Agricultural Sciences, Dharwad-580 005
Author
-
N. K. BIRADAR PATIL
University of Agricultural Sciences, Dharwad-580 005
Author
-
D. P. BIRADAR
University of Agricultural Sciences, Dharwad-580 005
Author
-
VILAS A. TONAPI
University of Agricultural Sciences, Dharwad-580 005
Author
Keywords:
Soybean, Seed longevity, SSR markersAbstract
Soybean [Glycine max (L.) Merrill] genotype varies in storability period during ambient storage. In this study, six genotypes of soybean, varying in storability, were characterized with 14 SSR markers. Out of 14 primers screened for seed longevity, the primer Satt 285, Satt 316, Satt 434 and Satt 538 gave distinct banding pattern. They produced an amplicon size range of 184 bp for those genotypes which are having poor seed longevity amongst the studied genotypes and the same loci produced amplicon size range up to 235 bp for those genotypes having good seed longevity.
Downloads
References
1. KARMARKAR PG, PS BHATNAGAR AND SM HUSAIN (1998). Identification of bold seeded soybean genotypes with good longevity in segregation generation of crosses. Soybean Genet. Newslett., 25: 76.
2. SAGHAI-MAROOF MA, KM SOLIMAN, RA JORGENSEN AND RW ALLARD (1984). Ribosomal DNA spacer length polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc. Nat. Academy Sci., USA, 81: 8014-8018.
3. CREGAN PB, T JARVIK, ALBUSH, RC SHOEMAKER, KG LARK, AL KAYA, TT VAN-TOAI, DG LOHNES, J CHUNG AND JE SPECHT (1999). An integrated genetic linkage map of the soybean genome. Crop Sci., 39: 1464-1490.
4. SINGH RK, RK RAIPURIA, VS BHATIA, R ANITA, PUSHPENDRA AND MS HUSAIN (2008). Identification of SSR markers associated with seed coat permeability and electrolyte leaching in soybean. Physiol. Mol. Biol. Pl., 14: 173-77.
5. KHARE D, P SAHU, N SAINI, AN SHRIVASTAVA AND M BHALE (2011). Soybean seed longevity. Paper presented in National Seed Congress, pp, 217-24.
6. JAGADISH H, MB ARUN KUMAR, A TALUKDAR, SK LAL AND MD ADLANI (2013). Molecular characterization and identification of candidate markers for seed longevity in soybean [Glycine max (L.) Merrill]. Indian J. Genet., 73: 64-71.
