Identification of rice varieties through chemical tests
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Keywords:
Standard phenol, modified phenol, NaOH, GA, 2, 4-D, varietal identificationAbstract
Variety identification has great significance from seed production, breeding as well as intellectual property rights point of view to ensure quality seed. Four varieties of rice were identified on the basis of seed colour (phenol, modified phenol and NaOH) and seedling response (GA, and 2, 4-D) to chemical tests. Though no individual chemical test was able to distinguish all the varieties, different chemical tests in conjunction were useful in identification of varieties. Karma Mahsuri exhibited negative response to phenol test and positive response to modified phenol test. However, three varieties namely, Danteshwari, Samleshwari and Mahamaya recorded positive response to both phenol and modified phenol test. Varieties Karma Mahsuri, Samleshwari and Mahamaya showed yellow colour reaction with NaOH test while, Danteshwari showed yellowish brown reaction. The effect of GA, and 2, 4-D on coleoptile growth of seedling was found to be variable among varieties studied.
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References
1. WALLS FW (1965). A standard phenol method for testing wheat for varietal purity. Hand Book of Seed Testing. AOSA, Contribution No.28.
2. BANERJEE SK AND CHANDRA R (1977). Modified phenol test for varietal identification of wheat. Seed Sci Technol, 5:53-66.
3. ANONYMOUS (1996). International rules for seed testing. Seed Sci Technol, 29:1-335.
4. JAISWAL JP AND AGRAWAL RK (1995). Varietal purity determination in rice: modification of the phenol test. Seed Sci Technol, 23:33-42.
5. ANONYMOUS (2007). PPV&FR authority specific DUS test guidelines for twelve notified crops-rice (Oryza sativa L). Pl Var J India, 1: 151-69.
6. PRAGYA KV BHAT, MISRA RL AND RANJAN JK (2010). Analysis of diversity and relationships among Gladiolus cultivars using morphological and RAPD markers. Ind J Agril Sci, 80: (9) 766-72.
7. "et al."