Solid Matrix Priming and Bio-agents Improve Seed Emergence in Okra [Abelmoschus esculentus (L.) Moench]


48 / 4

Authors

  • JAGADISH HOSAMANI Division of Seed Science and Technology, Indian Agricultural Research Institute, New Delhi 110012, IARI Regional Station, Karnal 13200 Author
  • V.K. PANDITA Division of Seed Science and Technology, Indian Agricultural Research Institute, New Delhi 110012, IARI Regional Station, Karnal 13200 Author
  • B.S.TOMAR Division of Seed Science and Technology, Indian Agricultural Research Institute, New Delhi 110012, IARI Regional Station, Karnal 13200 Author

https://doi.org/10.56093/sr.v38i2.164077

Keywords:

Okra, Priming, Field emergence, Bio-agents

Abstract

Rapid and uniform seedling emergence is a pre-requisite to better stand establishment and subsequent plant growth and yield in vegetable crops. One of the major problem of okra growers is difficulty in obtaining proper seedling emergence under low temperature. Since environmental factors often cannot be controlled, modification of the seed by either biological, chemical, or physiological techniques should be evaluated to improve the vigor of the seedling and uniformity of stand. Solid matrix seed priming (SMP) was performed by mixing seed with moist Vermiculite for 6, 12, 18, 24, 30, 36 and 48 h incubation at 20°C and 25°C. Based on seed germination, speed of germination and seedling vigour parameters, solid matrix priming for 24 h duration at 20°C was optimum for improving seed quality in okra cv Pusa A-4. Solid matrix priming followed by coating with Trichoderma viridae improved laboratory germination by 8.3% over unprimed seed. Coating seed with bio-agents like Trichoderma viridae and T. harzianum after SMP significantly improved speed of germination and seedling vigour. SMP followed by coating of Trichoderma viridae improved field emergence of okra seed by 33% over unprimed control. Field Emergence Index and 25 days old seedling growth and vigour was significantly higher in solid matrix primed seed coated with bio-agents. The results indicate that solid matrix priming along with coating of bio-agents like Trichoderma viridae and T harzianum improves field emergence and seedling growth in okra. SMP can be effectively employed to prime large amount of okra seed at one time. It is also cost effective as the medium can be reused many times.

Downloads

Download data is not yet available.

References

1. LORENZ, O.A., & MAYNARD, D.N. 1980. Knotts

handbook for vegetable growers. 2nd ed. New york:

Wiley.

2. STANDIFER, L.C., WILSON, P.W., & DRUMMOND,

A. 1989 The effect of seed moisture content on

hardseededness and germination in four cultivars of

okra (Abelmoschus esculentus (L.) Moench). Plant

Varieties and Seeds 2: 149-154.

3. MARSH, L. 1993 Moisture affects cowpea and okra

seed emergence and growth at low temperatures.

HortScience 28: 774-777.

4. DEMIR, I. 1997. Occurrence of hardseededness in

relation to seed development in okra (Abelmoschus

esculentus (L.) Moench). Plant Varieties and Seeds 10: 7-

13.

5. CONWAY, K.E. & MEREDDY, R. 2001. Beneficial effects

of solid matrix chemo-priming in okra. Plant Disease

85: 535-537.

6. PARERA, C.A. & CANTLIFFE, D.J. 1994. Pre-sowing

seed priming. Horticulture Review, 16: 109-141.

7. PILL, W.G. 1995 Low water potential and presowing

germination treatments to improve seed quality. In:

Basra, A.S. (ed) Seed Quality: Basic mechanisms and

agricultural implications.. Pp. 319-359. Food Product

Press, New York.

8. PANDITA, V.K., & NAGARAJAN, S. 2000.

Osmopriming of fresh seed and its effect on accelerated

ageing in Indian tomato (Lycopersicon esculentum Mill.)

varieties. Indian Journal of Agricultural Sciences 70: 479-

480.

9. PANDITA, V.K., ANAND, A. & NAGARAJAN, S. 2007.

Enhancement of seed germination in hot pepper

following pre-sowing treatments. Seed Science and

Technology, 35: 282-290.

10. EASTIN, J.A. 1990. Solid matrix priming of seed. U.S.

patent, 4: 874-912.

11. KHAN, A.A. 1992. Preplant physiological seed

conditioning. Horticulture Review, 14: 131- 181.

12. LIN, J.M. & SUNG, J. M. 2001 Pre-sowing treatments

for improving emergence of bitter gourd seedlings

under optimal and sub-optimal temperatures. Seed

Science & Technology 29: 39-50.

13. ISTA. 1993. International rules for seed testing. Rules

1993. Seed Science & Technology, 21 (supplement): 141-

168.

14. MAGUIRE, J.D. 1962. Speed of germination–aid in

selection and evaluation for seedling emergence and

vigour. Crop Science, 2: 176-177.

15. ABDUL-BAKI, A.A. & ANDERSON, J.D. 1973. Vigour

determination in soybean by multiple criteria, Crop

Sciences, 10: 31-34.

16. EGLI, D.B. & TEKRONY, D.M. 1985. Soybean seed

germination vigour and field emergence. Seed Science

and Technology, 23(3): 595-607.

17. CANTLIFFE, D.J., ELBALLA, M., GUEDES, A.,

ODELL, G.B., PERKINS-VEAZIE, P., SCHULTHESIS,

J.R., SEALE, D.N., SHULER, K.D., TANNE, I. &

WATKIN, J.T.1987. Improving stand establishment of

direct- seeded vegetables in Florida. Proc. Fla. State Hort.

Soc. 100: 213-216.

18. HEYDECKER, W., HIGGINS, J. & TURNER. Y.J., 1975.

Invigoration of seeds. Seed Science and Technology, 3: 881-

888.

19. HARRIS, D. 1996. The effects of manure, genotype, seed

priming, depth and date of sowing on the emergence

and early growth of sorghum in semi-arid Botswana.

Soil and Tillage Research, 40: 73-88.

20. NERSON, H., PARIS, H.S., KARCHI, Z. & SACHS, M.

1985. Seed treatments for improved germination of

tetraploid watermelon. HortScience, 20: 897-899.

21. DUNLAP, J.R. 1988. Effects of temperature and water

potential on the germination of muskmelon cultivars.

Annals of Applied Biology, 112: 187-194.

22. EDELSTEIN, M., CORBINEAU, F., KIGEL, J. &

NERSON, H. 1995. Seed coat structure and oxygen

availability control low-temperature germination of

melon (Cucumis melo) seeds. Physiologia Plantarum, 93:

451-456.

23. BRADFORD, K.J. 1986. Manipulation of seed water

relations via osmtic priming to improve germination

under stress conditions. Hort Science, 21: 1105-1112.

24. HEYDECKER, W. & COOLBEAR, P. 1977. Seed

treatments for improved performance: survey and

attempted prognosis. Seed Science and Technology, 5: 353-

425.

25. RUSH, C.M. 1991. Comparison of seed priming

techniques with regard to seedling emergence and

Pythium damping-off in sugar beet. Phytopathology, 81:

878-882.

26. BAIRD, R.E., NANKAM, C., MOGHADDAM, P.F. &

PATAKY, J.K., 1994. Evaluation of seed treatments on

shunken-2 sweet corn. Plant Diseases, 78: 817-821.

27. CALLAN, N.W., MATHRE, D.E. & MILLER, J.B. 1990.

Bio-priming seed treatment for biological control of

Pythium ultimum pre-emergence damping-off in sh-2

sweet corn. Plant Disease, 74: 368-372.

28. HARMAN, G.E. & TAYLOR, A.G. 1988. Improved

seedling performance by integration of biological

control agents at favorable pH levels with solid matrix

priming. Phytopathology, 78: 631-637.

29. HARMAN, G.E. & STASZ, T.E. 1989. Combining

effective strains of Trichoderma harzianum and solid

matrix priming to provide improved biological seed

treatment systems. Plant Disease, 72: 631-637.

30. COPELAND, L.O., BAALBAKI, R. & LEE, N.B. 1990.

The effect of seed treatment on laboratory and field

performance of soybean seed exposed to prolonged

wet, humid weather prior harvest. Journal of Seed

Technology, 14(1): 30-40.

31. SHERIF, A.F. 1953. Correlation of germination data of

corn and soybean lots under laboratory greenhouse and

field conditions. AOSA, 43: 127-130.

32. ANTHOW, K.L. & CALDWELL, R.M.1956. The

influence of seed treatment and planting rate on

emergence and yield of soybeans. Phytopathology, 46:

91-95.

33. SAXENA, N.P. 1987. Screening for adaptation to

drought case studied with chickpea and pigeon pea.

In: Adaptation of chickpea and pigeon pea to abiotic

stresses, proceedings of consultants workshop,

ICRISAT, Patancheru, India, December, 1984.

34. SAXENA, M.C. & SIDDIQUE, M. 1980. Survival of desi

chickpea in the population of Kabuli landraces 2.

International Chickpea Newsletter, 3: 6-7.

35. CLARK, G.E., HARMAN, T.J., KENNY & WATERS,

JR. 1980. Ralationship between the result of certain

laboratory tests and the field germination of soybean

seeds. Newsletter AOSA, 54: 36-43.

Downloads

Submitted

2025-01-24

Published

2025-01-29

How to Cite

JAGADISH HOSAMANI, V.K. PANDITA, & B.S.TOMAR. (2025). Solid Matrix Priming and Bio-agents Improve Seed Emergence in Okra [Abelmoschus esculentus (L.) Moench]. Seed Research, 38(2), 144-149. https://doi.org/10.56093/sr.v38i2.164077