Comparison of Biochemical and Molecular Markers Genetic Analysis in Niger [Guizotia Abyssinica (L.F.) Cass.] Genotypes
50 / 3
Authors
-
ZAKIR HUSSAIN
1 National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110 012, 2 Indian Agricultural Research Institute, New Delhi 110 012
Author
-
SANGITA YADAV
1 National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110 012, 2 Indian Agricultural Research Institute, New Delhi 110 012
Author
-
POONAM SUNEJA
1 National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110 012, 2 Indian Agricultural Research Institute, New Delhi 110 012
Author
-
S K YADAV
1 National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110 012, 2 Indian Agricultural Research Institute, New Delhi 110 012
Author
-
SHIV K YADAV
1 National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110 012, 2 Indian Agricultural Research Institute, New Delhi 110 012
Author
Keywords:
Genetic diversity, Guizotia abyssinica, Biochemical parameters, ISSR, Molecular markersAbstract
Variability in 35 accessions of niger (Guizotia abyssinica) was assessed for biochemical as well molecular markers. The biochemical parameters include test weight, oil and oil quality parameters. The analysis of variance showed significant differences among accessions for seed weight and oil parameters. Significant genetic diversity reported for test weight (0.44 g to 0.26 g), total oil content (40.47% - 35.16%), oleic acid (23.52-53.3%) and linoleic acid (32.03-58.28%). The range of Manhattan distance coefficient values based on biochemical parameters were 3.46 and 0.23 with a mean of 1.84. Molecular characterization was carried out using 25 ISSR primers. The range of mean genetic similarities between accessions based on Jaccard’s similarity coefficient was significantly high (0.1 to 0.95) with mean value of 0.40. The UPGMA clustering was done based on Manhattan distance coefficient for biochemical traits as well as Jaccard’s similarity coefficient values for ISSR markers. Although a significant genetic diversity was observed based on ISSR and biochemical markers, but only partial correlation between diversity based on these two parameters was observed. The comparison of genetic diversity based on both biochemical and molecular
markers has been made to find out the suitable accessions diverse for use in niger improment programs.
Downloads
References
1. MURTHY H N, HIREMATH S C AND SALIMATH S
S (1993). Origin, evolution and genome differentiation
in Guizotia abyssinica and its wild species, Theor Appl
Genet, 87: 587-592.
2. Hiremath S C & Murthy H N (1988). Domestication of
niger (Guizotia abyssinica). Euphytica,37: 225-228.
3. Almaw G & Teklewold A (1995). An agronomic and
seed quality evaluation of noug (Guizotia abyssinica
Cass.) germplasm in Ethiopia, Plant Breed, 114: 375-376.
4. Getinet A & Sharma S M (1996). Niger Guizotia abyssinica
(L.f) Cass. Promoting the conservation and use of
underutilized and neglected crops, (International Genetic
Resources Institute. Gatersleben, Germany).
5. Yadav S, Hussain Z, Suneja P, Nizar M A & Dutta M
(2012). Genetic divergence studies in niger (Guizotia
abyssinica) germplasm. Biomass and Bioenerg, 44: 64-69.
6. Yadav S, Kumar S, Hussain Z, Suneja P, Yadav S K,
Nizar M A & Dutta M (2012). Guizotia abyssinica (L.f.)
Cass.: An Untapped Oilseed Resource for the Future,
Biomass and Bioenerg, 43: 72-78.
7. Petros Y, Merker A & Zeleke H (2008). Analysis of
genetic diversity and relationships of wild Guizotia
species from Ethiopia using ISSR markers, Genet Resour
Crop Evol, 55: 451-458.
8. Hussain Z & Tyagi R K (2006). In vitro corm induction
and genetic stability of regenerated plants in taro
[Colocasia esculenta (L.) Schott], Indian J Biotechnol, 5:
535-542.
9. Sunil N, Sujatha M, Kumar V, Vanaja M, Basha S D &
Varaprasad K S (2011). Correlating the phenotypic and
molecular diversity in Jatropha curcas L, Biomass and
Bioenerg,35: 497-502.
