Seed conditioning as an effective method to alleviate imbibitional injury in some leguminous crop seeds
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Authors
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S.K. JAIN
National Bureau of Plant Genetic Resources, New Delhi 110 012
Author
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KALYANI SRINIVASAN
National Bureau of Plant Genetic Resources, New Delhi 110 012
Author
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A.D. SHARMA
National Bureau of Plant Genetic Resources, New Delhi 110 012
Author
Keywords:
Seed conditioning, imbibitional injury, legume seeds, germinationAbstract
Seeds in some leguminous crops viz, broadbean, chickpea (Brown), chickpea (Cream), cowpea, greengram, lentil and soybean conditioned to 9, 6 and 4% moisture contents were subjected to standard germination test. Results showed that cowpea and broadbean seeds were most susceptible to imbibitional injury. Soaking of these seeds in water for 16-20 hr prior to standard germination test resulted in a greater proportion of seeds failing to germinate, the degree of imbibitional injury being maximum in cowpea followed by broadbean. Seed conditioning (Slow rehydration) of these seeds to 17-21% moisture content was effective to mitigate the imbibitional damage. It is evident from the above study that the damage to the seeds following desiccation is more likely to be a consequence of imbibitional damage to which the seeds are more susceptible rather than a consequence of desiccation per se. The imbibitional damage can be mitigated to a large extent by slow conditioning of these legume seeds.
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References
1.ELLIS RH, HONG TD AND ROBERTS EH (1985). Hand Book of Seed Technology for Genebanks Vol. II. Compendium of Specific Germination Information and Test Recommendations. IBPGR Rome, pp 1-667
2. TITTEL C (1979). The germination of different qualities of soybean seeds under varying storage conditions. Bull Grain Technol 12: 3-10
3. ELLIS RH, OSEI-BONSU K AND ROBERTS EH (1982). Desiccation and germination of seed of cowpea [Vigna unguiculata (L.) Walp.]. Seed Sci Technol 10: 509-15
4. ANONYMOUS (2011). International rules for seed testing. Seed Sci Technol. ISTA Secretariat, Zurich, Switzerland
5. KOIZUMI M, KIKUCHI K, ISOBE S, ISHIDA N, NAITO S AND KANO Η (2008). Role of seed coat in imbibing soybean seeds observed by micro-magnetic resonance imaging. Ann Bot 102: 343-52
6. VERTUCCI CW AND LEOPOLD AC (1984). Bound water in soybean seed and its relation to respiration and imbibitional damage. Pl Physiol 75: 114-17
7. ISHIDA N, ΚΑΝΟ Η, KOBAYASHI T, HAMAGUCHI H AND YOSHIDA T (1988). The relationship between imbibitional damage and initial water content of soybean. 52: 2771-75
8. ISHIDA N, KΑΝΟ Η, ΚΟΒAYASHI T AND YOSHIDA T (1988). Analysis of physical states of water in soybean seeds by NMR. Agricultural and Biological Chem 52: 2777-81
9. SINGER SJ AND NICOLSON GL (1972). The fluid mosaic model of the structure of cell membranes. Science 175: 720-31
10. SAIZ L AND KLEIN ML (2002). Computer simulation studies of modern biological membranes. Accounts of Chemical Res 35: 482-89
11. BEMPORAD D, LUTTMAN C AND ESSEX JW (2005). Behaviour of small solutes and large drugs in a lipid bilayer from computer simulations. Biochemica et Biophysica Acta 1718: 1-21
12. ASHWORTH EN AND OBENDORF RL (1980). Imbibitional chilling injury in soybean axis: Relationship to stellar lesions and seasonal environments. Agron J 72: 923-28
13. MC DONALD MB JR, VERTUCCI CW AND ROOS EE (1988). Soybean seed imbibition: Water absorption by seed parts. Crop Sci 28: 993-97
14. LARSON LA (1968). The effect of soaking pea seeds with or without seed coat on seedling growth. Pl Physiol 43: 255-59
15. POWELL AA AND MATHEWS S (1981). A physical explanation for solute leakage from dry pea embryos during imbibition. J Expt Bot 32: 1045-50
16. POWELL AA (2006). Seed vigour and its assessment. In: Basra AS (ed) Handbook of Seed Science & Technology. Food Product Press, New York, pp 603-48
17. OBENDORF RL AND HOBBS PR (1970) Effect of seed moisture on temperature sensitivity during imbibition of soybean. Crop Sci 10: 563-66
18. KLINGMULLER W AND LANE GR (1960). Damaging effect of drying on Vicia faba seeds. Nature 185: 699-700
19. CHACHALIS D AND SMITH ML (2000). Imbibition behaviour of soybean [Glycine max (L) Merril] accessions with different testa characteristics. Seed Sci Technol 28: 321-31
20. NAKAYAMA N, HASHIMATO S, SHIMADA S, TAKAHASHI M, KIM YH, OYA T AND ARIHARA J (2004). The effect of flooding stress at the germination stage on the growth of soybean in relation to initial seed moisture. Japan J Crop Sci 73: 323-29
21. POLLOCK BM (1969). Imbibition temperature sensitivity of lima bean seeds controlled by initial seed moisture. Plant Physiol 44: 907-11
22. POLLOCK BM, ROOS EE AND MANALO JR (1969). Vigor of garden bean seeds and seedlings influenced by initial seed moisture, substrata, oxygen and imbibition temperature. J Am Soc Hort Sci 94: 577-84
23. NAKAYAMA N AND KOMATSU S (2008). Water uptake by seeds in yellow-seeded soybean [Glycine max (L) Merrill] cultivars with contrasting imbibition behaviors. Plant Production Science 11: 4415-22
24. CHACHALIS D AND SMITH ML (2001). Seed coat regulation of water uptake during imbibition in soybeans (Glycine max (L) Merr). Seed Sci Technol 29: 401-12.
