Effects of Electron Beam Irradiation on Germination and Vigour of Greengram (Vigna radiata) Seeds
48 / 3
Authors
-
SHASHI BHALLA
National Bureau of Plant Genetic Resources, New Delhi 110 012
Author
-
K. SRINIVASAN
National Bureau of Plant Genetic Resources, New Delhi 110 012
Author
-
SUBADAS SINGH
National Bureau of Plant Genetic Resources, New Delhi 110 012
Author
-
NEERAJ KUMAR
National Bureau of Plant Genetic Resources, New Delhi 110 012
Author
-
R. PRAMOD
National Bureau of Plant Genetic Resources, New Delhi 110 012
Author
-
S.C. BAPNA
National Bureau of Plant Genetic Resources, New Delhi 110 012
Author
-
J. DWIVEDI
National Bureau of Plant Genetic Resources, New Delhi 110 012
Author
-
ARUN SHARMA
National Bureau of Plant Genetic Resources, New Delhi 110 012
Author
-
R.K. KHETARPAL
National Bureau of Plant Genetic Resources, New Delhi 110 012
Author
Keywords:
Electron beam, irradiation, greengram, germination, vigourAbstract
The full genetic potential of a crop can be realised by pre-treatment of seeds with plant growth regulators and chemicals enhancing the seed germination and seedling vigour. Therefore, the present study was conducted to evaluate the effects of electron beam (EB) irradiation on greengram (Vigna radiata) seeds. For this purpose, dry seeds of greengram were exposed in a single layer to EB doses, viz. 170, 340, 510, 680, 850, 1020, 1190 and 1360 Gy at an energy level of 500 keV produced from a 750 keV, 20 kW DC accelerator. Irradiated seeds along with the control (unirradiated) were subjected to standard seed germination test and shoot length, root length, seedling vigour and vigour index. The seeds were also subjected to electrical conductivity and tetrazolium test for viability. Results showed that irradiation with EB doses as above have no significant effects on seed germination but affected seedling vigour and vigour index. The lower doses, viz. 170, 340 and 510 Gy had stimulatory effect on seedling vigour which were 37.69, 35.69, and 33.70 as compared to 30.19 in the control. Corresponding to this the seedling vigour index showed a decline @ 680 Gy. The electrical conductivity of seed leachate of the control and irradiated samples exhibited nearly a similar trend.
Downloads
References
1. PETWAL, V.C., R. PRAMOD, A. KAUL, S.C. ΒΑΡΝΑ & H.C. SONI (2004). Electron beam dosimetry study for 750 keV DC accelerator. INSAC-04.
2. ANONYMOUS (2008). International rules for seed testing. Seed Sci. Technol., ISTA, Zurich, Switzerland.
3. ABDUL-BAKI, A.A. & J.D. ANDERSON (1973). Vigour determination in soybean seed by multiple criteria. Crop Sci. 13: 630-633.
4. BHAT, R. & K.R. SRIDHAR (2008). Effect of electron beam irradiation on the quality characteristics of an economically valued tropical legume Mucuna pruriens L. Dc. EJAFA 7: 2578-2581.
5. KOTTAPALLI, B., C.E. WOLF-HALL, P. SCHWARZ & J. GILLESPIE (2003). Evaluation of hot water and electron beam irradiation for reducing Fusarium infection in malting barley. J. Food Prot. 66: 1241-1246.
6. WILSON, L.A., M.H. PERCHONOT & S.J.F.L. MARTIN (2007). Influence of low level irradiation of soybean on the quality of soyfood during mars mission. World of Food Science 2: 1-12 (www.worldfoodscience.org).
7. KIKUCHI, O.K., S. TODORIKI, M. SAITO & T. HAYASHI (2008). Efficacy of soft-electron (Low-energy Electron Beam) for soybean decontamination in comparison with gamma-rays. J. Food Science 63: 649-652.
8. TAKESHITA, H., Y. FUMIO & K. TAMIKAZU (2000). Radiation effect of low energy electron beam on plant growth. Food Irradiation 35: 59-63.
9. REDDY, P.V.R, S. TODORIKI, A. MIYANOSHITA, T. IMAMURA & T. HAYASHI (2006). Effect of soft electron treatment on adzuki bean weevil, Callosobruchus chinensis (L.) (Col., Bruchidae). J. Appl. Ent. 130: 393-399.
10. SITTON, J.W., J. BORSA, T.E. SCHULTZ & J.D. MAGUIRE (1995). Electron beam irradiation effects on wheat quality, seed vigor, and viability and pathogenicity of teliospores of Tilletia controversa and T. tritici. Plant disease, 79: 586-589.
