Characterization of Indian Rice (Oryza sativa L.) Varieties based on Seed Morphology and Phenol Colour Test
50 / 5
Authors
-
VIBHA KHANDELWAL
Division of Seed Science and Technology, Indian Agricultural Research Institute, New Delhi 110 012
Author
-
S.K. CHAKRABARTY
Division of Seed Science and Technology, Indian Agricultural Research Institute, New Delhi 110 012
Author
-
MALAVIKA DADLANI
Division of Seed Science and Technology, Indian Agricultural Research Institute, New Delhi 110 012
Author
-
S.P. SHARMA
Division of Seed Science and Technology, Indian Agricultural Research Institute, New Delhi 110 012
Author
Keywords:
Rice, varietal characterization, phenol test, seed morphologyAbstract
Characterization of 58 released Indian rice (Oryza sativa L.) varieties was done using seed morphological characteristics, viz, length, width, colour, shape, presence or absence of awn and phenol colour reaction. Twenty-six varieties were distinguishable individually and the rest were classified into discrete groups. The seed morphological characters based on IRRI and UPOV guidelines including kernel colour and grain length were compared. Phenol colour test, performed on seed from two years and harvest at different locations, confirmed that a specific colour reaction is predominant in each variety, though variation in the intensity of the colour was noticed. A reliable application of positive and negative colour response is suggested. Results suggested that prior to field based testing, these simple laboratory tests could provide for rapid grouping and facilitate verification of a large numbers of rice varieties.
Downloads
References
1. UPOV (1985). Guidelines for the conduct of test for distinctness, homogeneity and stability-rice (Oryza sativa L.). UPOV TG/16/4.
2. SINGHAL, N.C. & SURENDRA PRAKASH (1988). Use of phenol colour reaction on seed and outer glumes for identification of bread wheat cultivars. Plant Varieties and Seeds 1: 153-157.
3. AGRAWAL, P.K. (1992). Cultivar purity test. Agrawal P.K. & Dadlani M. (eds), In: Techniques in Seed Science and Technology. South Asian Publishers, New Delhi, pp. 172-173.
4. UPOV (2002). Guidelines for the conduct of test of distinctness, uniformity and stability-rice (Oryza sativa L.). UPOV TG/16/7 (Proj.), pp. 39.
5. IBPGR-IRRI (1980). Description of Rice (Oryza sativa L.). Los Banos, Philippines, pp. 18.
6. JANAIAH, K., K. KANT & S.P. SHARMA (2003). Use of phenol colour test for characterization of rice (Oryza sativa L.) varieties. Seed Res. 31(2): 141-147.
7. ΟΚΑ, Η. (1953). Phylogenetic differentiation of culminated rice plant. Variation in various characters and character combinations among rice varieties. Japan J. Breed. 3: 33-43.
8. OKA, H.L. (1958). Inter varietal variation and classification of cultivated rice. Indian J. Genet. 18: 79-89.
9. SIVASUBRAMANIAN, S. & V. RAMA KRISHANAN (1974). Use of phenol colour reaction for identification of rice varieties. Experimental Agriculture 10: 299-303.
10. SIVASUBRAMANIAN, S. & V. RAMA KRISHANAN (1978). Identification of rice varieties by laboratory techniques. Seed Res. 6: 71-76.
11. VANNANGAMUDI, K., V. PALANISWAMY & P. NATESAN (1988). Variety determination in rice-phenol and potassium hydrate tests. Seed Sci. Technol. 16: 465-470.
12. ELEKES, P. (1980). The nature and improvement of phenol reaction on wheat seeds. 19th ISTA Congress, Vienna, 6-13 June 1980. ISTA Reprint No. 13.
13. JOSHI, M.G. & S.K. BANERJEE (1970). Genetics of phenol colour reaction in emmer wheat, Proc. Intl. Seed Testing Assoc. 35(3): 807-811.
