Development of Seed Keys for Varietal Identification in Rice (Oryza sativa) using Chemical Tests
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Authors
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B. VIJAYALAKSHMI
Andhra Pradesh Rice Research Institute and RARS, Maruteru 534 122
Author
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D. VIJAY
Andhra Pradesh Rice Research Institute and RARS, Maruteru 534 122
Author
Keywords:
Rice, chemical tests, seed keys, varietal identification.Abstract
The decrease in the morphological variation among the major rice varieties due to narrow genetic base makes it necessary for the development of quick and reliable tests for varietal identification particularly for those working in seed certification and quality maintenance. Twenty three genotypes of rice were tested for five different chemical tests viz., Phenol, Modified Phenol, NaOH, FeSO, and KOH tests. Based on the colour reaction of different genotypes seed keys were developed for distinguishing the twenty three paddy varieties. All the five chemical tests grouped the varieties into two to four classes based on their reaction. Even though, not even a single test could identify any individual variety, but in conjunction the five chemical tests identified all the twenty three rice varieties individually. Thus these simple, reliable and quick tests can be used for varietal identification in rice crop.
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References
1. NAMRATA-GUPTA, M.A. JOSHI, N.K. SARAO & R.C. SHARMA (2007). Utility of phenol test in varietal characterization. Crop Improvement, 34(1): 77-81.
2. MOR, R.S., K.R. BATTAN & B.S. MEHLA (2006). Identification of rice varieties by laboratory techniques. National J. Pl. Improvement, 8(1): 28-31.
3. VARIER, A., M. DADLANI & S.P. SHARMA (1995). Phenol test and electrophoresis of seed esterases for testing genetic purity in seed lots of pearl millet (Pennisetum glaucum) inbreds. Indian J. Agri. Sci., 65(11): 789-792.
4. THANGAVEL, P., A. BHARATHI, N. NATARAJAN & T. EVERA (2005). Varietal grouping in sorghum by seed and seedling morphology and response to chemical testing. Karnataka J. Agri. Sci., 18(3): 664-672.
5. PALLARES, I.N., L.FERRARI, E.B. POSTULKA, M.N. RITTA (2004). Identification of cultivars of foxtail millet (Setaria italica (L.) Beauv.) from morphological and biochemical traits of their seed. Seed Sci. Technol., 32(1): 187-196.
6. WALLS, W.E. (1965). A standardized phenol method for testing wheat seed for varietal purity. AOSA contribution number 28.
7. BANERJEE, S.K. & R. CHANDRA (1977). Modified phenol test for varietal identification of wheat. Seed Sci. Technol., 5: 53-66.
8. GUPTA, P.Κ. & R.K. AGRAWAL (1987). Determination of varietal purity of paddy varieties by laboratory evaluation. Oryza, 25: 310-314.
9. VANANGAMUDI, K., V. PALANISAMY & P. NATESAN (1988). Variety determination in rice phenol and potassium hydroxide tests. Seed Sci. Technol., 16: 465-470.
10. AGRAWAL, P.K. & M. DADLANI (1987). Techniques in Seed Science and Technology, South Asian Publishers. New Delhi.
11. "et al."
