SDS-PAGE of Total Seed Protein in Relation to Cultivar Identification in Desi (G. arboreum) and American (G. hirsutum) Cotton


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Authors

  • R. C. PUNIA Department of Seed Science and Technology, CCS Haryana Agricultural University, Hisar 125 004 Author
  • PUNEET LUTHRA Department of Seed Science and Technology, CCS Haryana Agricultural University, Hisar 125 004 Author
  • ANITA MANN Department of Seed Science and Technology, CCS Haryana Agricultural University, Hisar 125 004 Author
  • C. RAM Department of Seed Science and Technology, CCS Haryana Agricultural University, Hisar 125 004 Author

https://doi.org/10.56093/sr.v35i1.165627

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References

1. ANONYMOUS (2006). Annual Report, All India Coordinated Cotton Improvement Project, (2005-06).

2. KAPSE, S.S. & Y.S. NERKAR (1985). Polyacrylamide gel electrophoresis of soluble seed proteins in relation to cultivar identification in cotton. Seed Sci. & Tech., 13: 847-852.

3. AGRAWAL, P.K., D. SINGH & M. DADLANI (1988). Identification of cotton hybrids seeds using PAGE. Seed Sci. & Tech., 16: 563-569.

4. DADLANI, M. & A. VARIER (1993). Electrophoresis for variety identification. Technical Bulletin. Division of Seed Sci. & Tech., ICAR, New Delhi.

5. IWASAKI, T., N. SHIBUYA, T. SUZUKI & S. CHIKUBU (1982). Gel filtration and electrophoresis of soluble rice proteins extracted from long, medium and short grained varieties. Cereal Chem., 59(3): 192-195.

6. SHEWREY, P.R., A.J. FANLKS, H.M. PRAT & B.J. MIFLIN (1978). The varietal identification of single seed of wheat by SDS-PAGE of gliadin. J. Sci. & Food Agri., 29: 847-849.

7. COOKE, R.J. & A.G. MORGAN (1986). A revised classification of barley cultivars using a standard reference electrophoresis method. J. National Inst. Agri. Bot., 17: 169-178.

8. SMITH, J.S.C. & R.D. WLYCH (1986). The identification of female selfs in hybrids of maize using electrophoresis and morphology. Seed Sci. & Tech., 14: 1-8.

9. VARIER, A., V. VASISHT & P.K. AGARWAL (1993). Identification of composite varieties of pearlmillet (Pennisetum ammicanum) using PAGE of total soluble proteins and isozymes. Seed Res. Spl., 1: 401-408.

10. KUMAR A., R.K. CHOWDHURY, R.L. KAPOOR & O.S. DAHIYA (1995). Identification of pearlmillet hybrids and their parental lines using seedling characters, chemical tests and gel electrophoresis. Seed Sci. & Tech., 23(1): 26-32.

11. VARIER, A., R. AGGARWAL, U. SINGH & S.P. SHARMA (1999). Characterization of castor (Ricinus commumis L.) hybrids and inbreeds by seed morphology and electrophoresis of seed proteins and isozymes. Seed Sci. & Tech., 27: 11-21.

12. WANG, Q.H., A.E. PERCIVAL & R.J. KOHEL (1990). Electrophoretic analysis of seed proteins in Gossypium herbaceum. Jiangsu. Jiangsu Agri. Sci., 5: 12-15.

13. ABDEL-TAWAB, F.M., E.M. FAHMY, M.O. ISMAIL & F.M. EL-DOMYATI (1993). Application of electrophoretic analysis in cultivar identification in cotton (Gozzypium barbadense L.). Annals Agri. Sci., Spl. Issue No. 2: 489-503.

14. DADLANI, M., V. VASHIST & A. VARIER (1994). Comparison of field grow out and electrophoresis methods for testing genetic purity of cotton hybrid seed. Seed Res., 22(2): 160-162.

15. COOKE, R.J. (1992). Handbook of variety testing. International Seed Testing Association, Switzerland, pp. 2-7.

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Submitted

2025-03-05

Published

2025-03-06

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Short Communication

How to Cite

R. C. PUNIA, PUNEET LUTHRA, ANITA MANN, & C. RAM. (2025). SDS-PAGE of Total Seed Protein in Relation to Cultivar Identification in Desi (G. arboreum) and American (G. hirsutum) Cotton. Seed Research, 35(1), 102-105. https://doi.org/10.56093/sr.v35i1.165627