Identification of Lentil Varieties by Seed Protein Electrophoresis
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Authors
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KANCHAN SINGH
Seed Technology Research Unit, Department of Plant Breeding and Genetics JNKVV, Jabalpur-482 004
Author
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D. KHARE
Seed Technology Research Unit, Department of Plant Breeding and Genetics JNKVV, Jabalpur-482 004
Author
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S. RAO
Seed Technology Research Unit, Department of Plant Breeding and Genetics JNKVV, Jabalpur-482 004
Author
Keywords:
Lentil, protein banding, similarity indexAbstract
Twenty five varieties of lentil obtained from IIPR, Kanpur were analyzed for varietal identification through electrophoresis of seed protein. In all 15 bands were recorded with maximum relative mobility of 0.90. Presence or absence of any particular band helps in demarcation and identification of a variety. Similarity index was calculated to have an idea about evolutionary relationship among varieties in this experiment which varied from 53.3% to 93.3%.
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References
1. PATHAK, A.K. & N.C. CHHATTOPADHYA (1989). Characterization of jute (Corchorus spp.) cultivars by polyacrylamide gel electrophoresis soluble seed proteins. Indian J. Expl. Blol., 27: 196-197.
2. DADLANI, M. & A. VARIER (1993). Electrophoresis for variety identification. Tech. Bull., Division of Seed Science and Technology, IARI, New Delhi.
3. HUSAIN A., W. BHUSHUK & K.W. CLARK (1989). Discrimination of cultivars of lentil (Lens culinaris Medic) by electrophoresis of seed proteins. Can. J. Plant Sci., 69: 243-246.
4. GOMATHINAYAGAM P. & N.M. RAMASWAMY (1994). Seed protein pattern of cowpea (Vigna unguiculata L.) and its distant species. J. Plant Biochem. & Biotechnol., 3 : 149-151.
5. GOYAL, K. C. & S. N. SHARMA (1998). Biochemical tools (Discontinuous PAGE) for varietal identification in soybean (Glycine max L.). Seed Res., 26: 204-206.
6. MISHRA, S. K., B. SHARMA, S.K. DASGUPTA & R. KAHLON (1996). Electrophoretic variation for seed protein of pea (Pisum sativum L) genotypes. Seed Res., 24(2): 88-92.
