Tuber Protein Electrophoregram as a Tool of Genetic Purity Test in Potato (Solanum tuberosum L.)*
14 / 11
Authors
-
A.C. MISHRA
Department of Vegetable Science G.B. Pant University of Agriculture and Technology, Pantnagar-263 145
Author
-
N.P. SINGH
Department of Vegetable Science G.B. Pant University of Agriculture and Technology, Pantnagar-263 145
Author
-
J.P. MISHRA
Department of Vegetable Science G.B. Pant University of Agriculture and Technology, Pantnagar-263 145
Author
Keywords:
Protein, Electrophoresis, Potato, variabilityAbstract
In this investigation 38 potato (Solanum tuberosum L.) genotypes were used for morphological characterization and tuber protein electrophoresis. Results indicated that all 38 genotypes could be categorized into 14 distinct groups consisting of morphologically almost identical genotypes. The morphological ambiguity was distinguished through electrophoregrams emphasizing presence or absence and intensity of bands. For example, the electrophoregram of morphologically similar genotypes Kufri Lalima and JP-100 were quite distinct in regard to B3, B4, B6 & B7 that were additional in JP-100. Similarly, other genotypes with common morphology could be differentiated. The protein bands in molecular weight range of 68 kD-40 kD exhibited maximum variability across the genotypes. The tuber protein profiles of all randomly sampled tubers in lot of each genotype were identical. Thus, the SDS-PAGE of tuber protein appeared as a good tool for genetic analyses, breeding, establishing relationships, classification and testing of genetic purity in potato.
Downloads
References
1 HOSAKA, K. & M. MATSUBAYASHI (1983). Studies on physiologenetic relationship in tuberous Solanum by isozyme analysis. Phylogenetic relationship between Mexican and South American diploid species. Science Reports of Faculty of Agriculture, Kolbe Univ., 15:217-228.
2. OLIVER, J.L. & J.M. MARTINEZ-ZAPATER (1984). Allozyme variability and phylogenetic relationship in cultivated potato (Solanum tuberosum L.) and related species. Plant Systematics and Evol., 148: 1-18.
3. OLIVER, J.L. & J.M. MARTINEZ-ZAPATER (1985). A genetic classification of potato cultivars based on allozyme patterns. Theor. Appl. Genet. 69: 305-311.
4. HUAMAN, Z. & H. STEGEMANN (1989). Use of electrophoretic analysis to verify morphologically identical clones in a potato collection. Plant Varieties and Seed. 2: 155-161.
5. CHOI, D.R., I.G. MOK & H.Y. KIM (1991). Identification of potato cultivars and clones by electrophoresis patterns of tuber protein and isozymes. J. Korean Soc. Hort. Sci., 32: 23-28.
6. HOSAKA, K. & R.E. HANNEMAN, Jr. (1991). Seed protein variation within accessions of wild and cultivated potato species and inbred Solanum chacoense. Potato Res., 34: 419-428.
7. DOUCHES, D.S. & K. LUDLAM (1991). Electrophoretic characterization of North American poato cultivars. Amer. Potato J. 68: 767-780.
8. LE, C.L. (1994). Use of electrophoresis in identification of potato varieties grown in Switzerland. Revue Suisse d' Agriculture. 26(6): 373-379.
9. COOK, R.J. (1995). New approaches to potato variety identification. Potato Res. 42: 529-539.
10. BINSFELD, P.C., J.A. PETERS & E. AUGUSTIN (1996). Isoenzymatic variation in potato somaclones (Solanum tuberosum L.). Brazilian J. Genet. 19(1): 117-121.
11. JOPIA, Q. & M. JOSE (1997). Classification of potato clones (Solanum tuberosum L. ssp tuberosum Hawkes) using protein electrophoresis. Valvidia, Chile, 65p.
12. SUH, E.J. & S.G. SUH (1997). Comparison of wound-induced proteins in potato by two-dimensional gel electrophoresis. J Korean Soc. Hort. Sci. 38 (2): 133-136.
13. HARDING, K. (1994). Molecular stability of the ribosomal RNA gene in Solanum tuberosum plants recovered from slow growth and cryoconservation. Euphytica. 55:141-146.
14. PIOLA, F., R. ROHR & P. HEIZMANN (1999). Rapid detection ni genetic variation within and among in vitro propagated codar (Cedrus libani Loudon) clones. Plant Science 141: 159-163.
15. BOURQUE, J.E., J.C. MILLER & W.D. PARK (1987), Use of an in vitro tuberization system to study tuber protein gene exprezion In: Vitro Cellular and Developmental Biol. 23: 381-386.
16. RAJAPAKSE, D.P., T. IMAI & T. ISHIGE (1991). Analysis of potato microtuber proteins by sodium dodecylsulfate polyacrylamide gel electrophoresis. Potato Res., 34: 285-293.
17. KAPOOR, A.C., S.L. DESBOROUGH & P.M. LL. (1975). Potato tuber proteins and their nutritional quality. Potato Res., 18:469-478.
18. STEGEMANN, H. & V. LOESCHCKE (1976). Index of European potato varieties. Mitteilungen aus der Biologischen Bundesanstalt fur land-und Forstwirtschaft Berlin-Dahlem. 168: 1-214.
19. PARK, W. D., C. BLACKWOOD, C. A. MIGNERY, M. Α. HERMODSON & R. M. LISTER (1983). Analysis of the heterogeneity of 40,000, molecular weight tuber glycoprotein of potatoes by immunological methods and NH2-terminal sequences analysis. Plant Physiol. 71: 156-160..
20. BARTA, J., V. CURN & J. DIVIS (2003). Study of biochemical variability of potato cultivars by soluble proteins. Plant Soil Environ. 49 (5): 230-236.
21. LAEMMLI, U.K. (1970). Cleavage of structural protein during assembly of the head of bacteriophage T4. Nature, 227: 680-685.
22. BRADFORD, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochem. 72: 248-254.
23. SMITH, J.S.C. & O.S. SMITH (1992). Finger printing of crop varieties. Adv. Agron. 47: 87-100.
24. UPOV (1996). Model Law on the Potection of New Varieties of Plants, ii. International Convention for the Protection of New Varieties of Plants 1991, UPOV, Geneva.
25. MACIAS, M.M., T.R. MANCILLA & MA. CONTRERAS (1989). Identification of Chilean potato (Solanum tuberosum ssp. tuberosum) by protein and esterase electrophoresis. Agro Sur. 17(1): 56-63.
26. SYRKOVA, S. (1999). Identification of potato (Solanum tuberosum) varieties using electrophoresis of proteins and enzymes. Rostlinna Vyroba. 45(4): 193-196.
