Studies on Genetic Diversity in Chickpea (Cicer arietinum L.) using total Seed Protein Separation by SDS-PAGE
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Authors
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ANITA MANN
CCS Haryana Agricultural University, Hisar-125004
Author
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B.S. DAHIYA
CCS Haryana Agricultural University, Hisar-125004
Author
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A.S. NANDWAL
CCS Haryana Agricultural University, Hisar-125004
Author
Keywords:
chickpea, SDS-PAGE, seed protein, genetic relationshipAbstract
Thirty Indian cultivated chickpea genotypes were investigated to study the genetic diversity. All the genotypes were separated and compared by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Relative intraspecific similarities of protein patterns were estimated by Nei and Li equation and a cluster analysis was performed. A total of 39 bands were observed with Rm value ranging from 0.11 to 0.95. A minimum of 14 and a maximum of 34 bands were observed. The kabuli varieties have more high molecular weight proteins than the desi genotypes. Protein content was highest (0.2394 mg/g seed) in one kabuli genotype, HK-89-131 and one desi genotype, H-92-67 and minimum in GL-94022 (0.0194 mg/g seed). Two broad clusters of all chickpea genotypes were obtained in the dendrogram at a dissimilarity value of 25%.
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References
1. KIMBER, G. (1984). Evolutionary relationships and their influence on plant breeding. In: Gustafson J.P. (Ed.) Gene manipulation in plant improvement. Plenum Press, New York, pp 281-300.
2. MARGOLIASH, E. & W. M. FITCH (1968). Evolutionary variability of cytochrome "c" primary structures. Ann. NY. Acad. Sci. 151: 359-381.
3. SAMMOUR, R. H. (1992). An electro phoretic analysis of the variation in the seed storage proteins of Vicia faba L. cultivars. Feddes repertorium. 102(7-8): 555-557.
4. VAIRINHOS, F. & D. R. MURRAY (1983). The seed proteins of chickpea: comparative studies of Cicer arietinum, C. reticulatum and C. echinospernum (Leguminosae). Plant Syst. Evol. 142: 11-22.
5. LADIZINSKY, G. & T. HYMOWITZ (1979). Seed protein electrophoresis in taxonomic and evolutionary studies. Theor. Appl. Genet. 54: 145-151.
6. LADIZINSKY, G. & B. L. JOHNSON (1972). Seed protein homologies and the evolution of polyploidy in Avena. Canadian J. Genet. Cyto. 14: 875-888.
7. COOKE, R.J. (1992). Handbook of variety testing. Electrophoresis book: Variety identification. International Seed Testing Association; Zurich; Switzerland, 46 pp.
8. DADLANI, M., V. VASHISHT, & A. VARIER (1994). Comparison of field growout and electrophoresis methods for testing the genetic purity of cotton hybrid seed. Seed Res. 2(22): 160-162.
9. DADLANI, M. & A. VARIER (1993). Electrophoresis for variety identification. Technical Bulletin. Div. Seed Sci. Technol., ICAR, New Delhi.
10. DADLANI, M. (1998). Use of biochemical markers for testing the identity and purity of a cultivar. Seed Tech. News. 28(4): 45.
11. SINGH, H.P., P. V. SINGH, & R. P. SAXENA (1991). Identification of chickpea cultivars using PAGE of storage seed protein. Annal Biol. 7(2): 167-175.
12. KHARKWAL, M. C. (1999). Seed Storage proteins and inter specific relationship in Cicer arietinum L. Indian J. Genet. Plant Breed. 59: 59-64.
13. SATIJA, D. R., A. BALA, V. P. GUPTA & S. K. GUPTA (1996). Genetic improvement for protein content and quality in chickpea (Cicer arietinum L.). Crop Imp. 23: 139-146.
