Dose Dependent Impact of Dominant Seedborne Fungi on Seed Germination and Seedling Vigour of Cotton Seeds
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Authors
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R. KAVITHA
Department of Applied Botany, Seed Pathology and Biotechnology, University of Mysore, Manasagangotri, Mysore-570 006
Author
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S. UMESHA
Department of Applied Botany, Seed Pathology and Biotechnology, University of Mysore, Manasagangotri, Mysore-570 006
Author
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H.S. SHETTY
Department of Applied Botany, Seed Pathology and Biotechnology, University of Mysore, Manasagangotri, Mysore-570 006
Author
Keywords:
Cotton genotypes, Pathogenic fungi.Abstract
Thirty cotton genotypes belonging to Varalakshmi, Jayalakshmi, DCH-32 and RCH-32 were collected from the three different agro climatic zones of Karnataka state, India. All the collected seed samples were manually delinted and subjected to standard blotter method to screen the seedborne fungi. The dominant seedborne pathogenic fungi were identified and multiplied on potato dextrose agar media. Seeds were artificially treated with all the dominant seedborne fungi. Fungal concentration was assessed using Haemocytometer. Such artificially treated seeds were subjected to seed germination and seedling vigor. All the dominant seedborne fungi reduced seed quality parameters significantly (p=0.05), over their untreated counterparts. However, the effect of these seedborne pathogenic fungi is dependant on fungal dosage. Increased fungal concentration recorded more reduction in seed quality parameters. But the fungal dosage beyond 150-200 x 10 CFUgm did not vary significantly (p=0.05). Among the fungi tested, the highest reduction of seed quality parameters recorded from Verticillium dahliae followed by Myrothecium roridum; Macrophomina phaseolina and Fusarium species.
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References
1. ANONYMOUS (2004). Survey of Indian Agriculture, The Hindu M/S, Kasturi and sons Ltd, National Press, Kasturi buildings, Chennai-600 002, p 36-80.
2. RAO, Y.P. & M. K. MESHRAM (1983). Studies on bacteriophages of Xanthomonas oryzae, the incitant of bacterial blight of rice and understanding the epidemiology. Ph. D. Thesis, Indian Agriculture Research Institute, New Delhi, India.
3. BHATAWDEKAR, K. M., P. JOSHI & R.P. BHARADWAJ (1999). Chemical Composition of cotton seeds. J. Indian Soc. Cotton Improv. 43: 226-325.
4. ANONYMOUS (2003). Assessment of impact and main dynamics of cotton disease affecting in particular-small-scale production systems in Southern and Eastern Africa. Final report/CAF, May-2003.
5. RAMACHANDRA, T.V., G, KAMAKSHI & B.V. SHRUTHI (2004). Bioresource status in Karnataka. Ren. Sustainable Energy Rev. 8: 1-47.
6. ISTA (2003). International Rules for seed Testing, Rules 2003. (Draper SR, ed.) Zurich, Switzerland: International Seed Testing Association. p. 1-520.
7. BOOTH, C. (1977). Fusarium, A Laboratory Guide to the Identification of the major species. Common Wealth Agriculture Bureau, Furnham Royal, England, p 5-43.
8. SINGH, K., J.C. FRISVAD. V. THRARS & S.B. MATHUR (1991). An Illustrated Manual on Identification of Some Seed-borne Aspergilli, Fusaria, Penicillia and their mycotoxins. Danish Government of Seed Pathology for Developing Countries, Denmark.
9. MATHUR, S.B., & O. KONGSDAL (2003). Common Laboratory Seed Health Testing Methods for Detecting Fungi. International Seed Testing Association, P.O. Box 308, 8303, Bassersdorf, CH-Switzerland. p 202-271.
10. ABDUL BAKI, A.A. & J.D. ANDERSON (1973). Vigour determination in Soybean seed by multiple criteria. Crop Sci. 13: 630-33.
11. ABIBALENTJA, N. & D.M. EASTBURN (2000). Influence of inoculum density of Verticillium dahliae in root discolouration of Horseradish. Plant Dis. 84(3).
12. GOUR, H.N., J. ASHIYA & R. NATH (2001). Effect of bacterial inoculum (Xanthomonas axonopodis pv. vignicola) on seed germination, fresh and dry weight of cowpea (Vigna unguiculata (L.) walp.) seedlings. J. Mycol. Plant Pathol. 31(1), 46-49.
