Protein Composition of Cotton Seeds in Relation to Agro-Climatic Variation
104 / 82
Keywords:
Cotton, seed protein, tris-soluble protein, storage fraction, globulin, albuminAbstract
Cotton, globally grown as a fibre crop, is also popular as oil and protein source, which is obtained from cotton seed. An investigation on seed protein content was done in popular cotton cultivars released by ICAR– Central Institute for Cotton Research. Twelve cultivars belonging to three cultivated species (G. hirsutum, G. arboreum and G. barbadense), seeds of which were produced in different locations (Central, North, and South cotton growing regions), were subjected to determination of tris-soluble and seed storage protein fractions. The laboratory investigation was conducted at one location after collecting seeds of same genotypes grown in other locations in the same season for comparing their protein content. Concentration of tris-soluble protein and sequentially extracted storage protein fractions were determined by Bradford assay. Significant variation was observed for tris-soluble and storage protein fractions among genotypes in each location and showed varied relations with seed index in G. arboreum and G. barbadense. Among the storage fractions, globulin fraction was highest followed by albumins in the studied cultivars. Though the values of each protein component differed widely in different location, the genotypes maintained their rank for both tris-soluble protein content as well as seed storage fraction content irrespective of the location where the seeds were produced.
Downloads
References
1. QING L, D LLEWELLYN, SP SINGH, AND AG GREEN (2012). Cotton seed development: opportunities to add value to a byproduct of fibre production, Cotton Flowering and Fruiting, chapter 9, 134-161.
2. MUTHUSAMY A AND N JAYABALAN (2013). Variations in seed protein content of cotton (Gossypium hirsutum L.) mutant lines by in vivo and in vitro mutagenesis. Journal of Environmental Biology, 34: 11-16.
3. GUIZHEN L, H MEI, S WANG, X LI, X ZHU, AND T ZHANG (2015). Association mapping of seed oil and protein contents in upland cotton. Euphytica, 205: 637–645.
4. BHAT RS, RV HUNJE AND SS BHARATH (2024). Fresh seed dormancy in groundnut: Mechanisms, Factors and Implications for Crop Improvement – a Review Seed Research, 52: (1) 1-8.
5. CHOUGALA LD, PARASHIVMURTHY, MS HARISH AND R SIDDARAJU (2024). Seed Polymer Coating with ZnO, SiO2 and TiO2 Nanoparticles: An Innovative Seed Quality Enhancement Technique in Sweet Pepper. Seed Research, 52(2): 124-129.
6. POOJA, SR PUNDIR, O SANGWAN, M RATHI, S KUMAR, AND DIGAMBER (2020). Study of genetic parameters and clustering on the basis of morpho-bio traits of cotton genotypes Journal of Cotton Research and Development, 34(2): 183-191.
7. BHATTI KH, Y BOLEK, H TEKEREK, AND A BARDAK (2016). Screening of Cotton Genotypes for Protein Content, Oil and Fatty Acid Composition. Journal of Agriculture Science, 8(5): 107-121.
8. BELLALOUI N, RB TURLEY, AND SR STETINA (2021). Cottonseed Protein, Oil, and Minerals in Cotton (Gossypium
hirsutum L.) Lines Differing in Curly Leaf Morphology. Plants,10: 525. https://doi.org/10.3390/ plants10030525.
9. RANJAN S, R MATCHA, B MADHURI, AND NR BABU (2012). Comparative evaluation of protein extraction methods from few leguminous seeds. International Journal of Advanced Biotechnology and Research, 3(2) pp 558-563.
10. BRADFORD M (1976). A rapid and sensitive method for the quantitation of microgram quantities.
11. PANDA AM, R MISHRA, A KUMAR, A KERKETTA, AND N SONI (2018). Seed storage protein evaluation of few rice varieties used by tribal people of Chhattisgarh. Journal of Rice Research, 11(2):31-35.
12. VIDYA CJ AND N NETHRA (2024). Seed Priming: Tool towards Sustainable Agriculture. Seed Research, 52(1): 30-40.
13. WU M, W PEI, T WEDEGAERTNER, J ZHANG, AND J YU (2022). Genetics, Breeding and Genetic Engineering to Improve Cottonseed Oil and Protein: A Review. Front Plant Sci. 10(13): 864850.
14. SINGH AND KAUR (2019). Comparative studies on seed protein characteristics in eight lines of two Gossypium species
Journal of Cotton Research, 2: 6 https://doi.org/10.1186/s42397-019-0024-3
15. HINZE L, P HORN, N KOTHARI, J DEVER, J FRELICHOWSKI, K CHAPMAN, AND R PERCY (2015). Nondestructive Measurements of Cottonseed Nutritional Trait Diversity in the U.S. National Cotton Germplasm Collection. Crop Science, 55, pp 770-782.
16. SAMMOUR RH, MN EL-SHOURBAGY, AM ABO-SHADY, AND AM ABASARY (1995). Proteins of cottonseed (Gossypium barbadense) extraction and characterization by electrophoresis. Qatar Univ. Sci. J., 15(1): 77- 82.
17. FLATIE D, T TESFAYE, KM BABU, M GIBRIL, AND F KONG (2023). Extraction and characterization of protein from
cottonseed meal. Environ Waste Management Recycling, 6(2): 140.
18. PARKASH V, JL SNIDER, A BRUCE, A ERMANIS, G VIRK, N KAUR, G COLLINS, AND KD CHAPMAN (2023). Effects of cultivar and nitrogen application rate on lint, seed, oil, and protein yields of field-grown cotton. Crop Science, 63(3): 1541-1554.
19. MERT M, Y AKISCAN, AND O GENCER (2004). Inheritance of Oil and Protein Content in Some Cotton Generations. Asian
Journal of Plant Sciences, 3: 174-176.