Storage Duration Impacted Hardseededness and Seed Coat Physico-chemical Parameters in Mung bean (Vigna radiata L.)
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Authors
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DEBASHIS PAUL
Division of Seed Science and Technology, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India
Author
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SK CHAKRABARTY
Division of Seed Science and Technology, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India
Author
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LATA NAIN
Division of Microbiology, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India
Author
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PRATIK DHAKADE
ICAR-Central Institute for Cotton Research, Nagpur, Maharashtra-441108, India
Author
Keywords:
Hardseededness, Lignin content, Hardness breaking force, Storage duration, Green gramAbstract
Mung bean (Vigna radiata (L.) Wilczek) is an important pulse crop of India; however, hardseededness
poses a major constraint to its germination and grain quality. The present study investigated the kinetics of
hardseededness and associated biochemical changes in six hard-seeded mung bean genotypes (TM 96-25,
LGG 460, PUSA 9072, MH 421, V 61-73, PUSA 9531) stored for 6, 12, and 18 months under ambient conditions.
Key parameters measured included per cent hard seed, hardness breaking force (N), Acid Insoluble Lignin%
(AIL%), Acid Soluble Lignin% (ASL%), and total phenol content (mg/g). Results revealed a significant reduction in
hardseededness with increased storage duration. The mean hard seed percentage decreased from 31.73% in
fresh seeds to 11.61% after 18 months. Concurrently, the parameters such as Acid Insoluble Lignin (%), Acid
Soluble Lignin (%), hardness breaking force (N) and total phenol content (mg/g) significantly declined over the
storage period, indicating a weakening of the seed coat. This change is likely attributed to alteration in membrane
integrity and loosening of hilum cells, which enhances water permeability. While 18-month storage reduced
germination below Indian minimum seed certification standards, 12-month stored seeds maintained acceptable
quality. Therefore, utilizing 12-month stored seeds of hard-seeded mung bean genotypes is recommended for
improved field emergence, uniform plant growth, and higher yields in seed production programs.
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