The New Lens on Seed Testing: AI, ML and Image Analysis


436

Authors

  • LAKSHMIPRASANNA KATA Dept. of Seed Science & Technology, SRTC campus, Professor Jayashankar Telangana Agricultural University, Rajendranagar, Hyderabad, Telangana-500030, India Author
  • APARNA KUNA MFPI-Quality Control Laboratory, EEI campus, Professor Jayashankar Telangana Agricultural University, Rajendranagar, Hyderabad, Telangana-500030, India Author
  • PALLAVI MANDALAPU Dept. of Seed Science & Technology, SRTC campus, Professor Jayashankar Telangana Agricultural University, Rajendranagar, Hyderabad, Telangana-500030, India Author
  • BHARATHI YERAS Agricultural Research Station, Tandur, Professor Jayashankar Telangana Agricultural University, Rajendranagar, Hyderabad, Telangana-500030, India Author
  • SUJATHA PATTA Dept. of Genetics and Plant Breeding, Agricultural College, Polasa, Jagtial, Professor Jayashankar Telangana Agricultural University, Rajendranagar, Hyderabad, Telangana-500030, India Author
  • ZUBEDA SOHAN MFPI-Quality Control Laboratory, EEI campus, Professor Jayashankar Telangana Agricultural University, Rajendranagar, Hyderabad, Telangana-500030, India Author

https://doi.org/10.56093/sr.v53i2.7

Keywords:

Digital seed testing, artificial intelligence, image analysis, seed quality, hyperspectral imaging, machine learning

Abstract

Seed testing is undergoing a paradigm shift with the integration of advanced digital technologies
that augment conventional methodologies by offering greater speed, accuracy, and scalability. This review examines
the transformative impact of innovations such as automation, artificial intelligence (AI), and advanced image
analysis on contemporary seed quality assessment. AI-based approaches, particularly machine learning and
deep learning models, are increasingly being employed to automate complex and labour-intensive tasks—including
seed sorting, germination prediction, and defect detection—that were traditionally reliant on expert visual evaluation.
Image analysis techniques, notably hyperspectral and multispectral imaging, enable rapid and non-destructive
assessment of both external and internal seed attributes, thereby substantially improving diagnostic precision. In
parallel, the adoption of edge computing, Internet of Things (IoT) sensors, and cloud-based platforms has enabled
real-time and decentralized seed testing, extending analytical capabilities from centralized laboratories to fieldlevel applications. These digital frameworks enhance data integration, traceability, and decision-making efficiency
across seed production systems, breeding programmes, and quality control laboratories. Moreover, high-throughput
digital phenotyping tools are accelerating plant breeding by generating detailed, high-resolution trait data, thereby
reducing selection cycles and improving the efficiency of crop improvement programmes. Despite these
advancements, the widespread adoption of digital seed testing technologies necessitates the development of
standardized protocols, robust validation frameworks, and appropriate regulatory oversight to ensure data reliability,
interoperability, and broader applicability. Collectively, these emerging innovations are redefining seed testing
practices and positioning the discipline as a critical enabler of sustainable, precision-oriented, and data-driven
agriculture.

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References

1. KUMAR A, S SINGH AND N MEHTA (2022). Applications of

artificial intelligence and image analysis in seed quality testing.

Journal of Agricultural Informatics, 13(1): 10-20.

2. COLMER J, CM O NEILL, R WELLS, A BOSTROM, D

REYNOLDS, D WEBSALE (2020). SeedGerm: a cost-effective

phenotyping platform for automated seed imaging and

machine-learning based phenotypic analysis of crop seed

germination. New Phytologist, 228(2): 778-793.

3. FENG L, S ZHU, F LIU, Y HE, Y BAO AND C ZHANG (2019).

Hyperspectral imaging for seed quality and safety inspection:

a review. Plant Methods, 15: 91.

4. BHARGAVA A, A SACHDEVA, K SHARMA, MH ALSHARIF, P

UTHANSAKUL AND M UTHANSAKUL (2024). Hyperspectral

imaging and its applications: a review. Heliyon, 10(12): e33208.

5. GUO D, Q ZHU, M HUANG, Y GUO AND J QIN (2017). Model

updating for the classification of different varieties of maize

seeds from different years by hyperspectral imaging coupled

with a pre-labeling method. Computers and Electronics in

Agriculture, 142: 1-8.

6. SUN J, S JIANG, H MAO, X WU AND Q LI (2015).

Classification of black beans using visible and near infrared

hyperspectral imaging. International Journal of Food

Properties, 19(8): 1687-1695.

7. YU Z, H FANG, Q ZHANGJIN, C MI, X FENG AND Y HE (2021).

Hyperspectral imaging technology combined with deep

learning for hybrid okra seed identification. Biosystems

Engineering, 212: 46-61.

8. AN D, L ZHANG, Z LIU, J LIU AND Y WEI (2023). Advances in

infrared spectroscopy and hyperspectral imaging combined

with artificial intelligence for the detection of cereals quality.

Critical Reviews in Food Science and Nutrition, 63(29): 9766-

9796.

9. ZHAO X, H QUE, X SUN, Q ZHU AND M HUANG (2022).

Hybrid convolutional network based on hyperspectral imaging

for wheat seed varieties classification. Infrared Physics and

Technology, 125: 104270.

10. LI B, J SUN, Y LIU, L SHI, Y ZHONG AND P WU (2024).

Multi-level data fusion strategy based on spectral and image

information for identifying varieties of soybean seeds.

Spectrochimica Acta Part A: Molecular and Biomolecular

Spectroscopy, 322: 124815.

11. SUGANTHI M AND JGR SATHIASEELAN (2022). A novel

feature extraction method for identifying quality seed selection.

International Journal of Intelligent Engineering, 8(1): 1-10.

12. GHIMIRE A, SH KIM, A CHO, N JANG, S AHN, MS ISLAM

(2023). Automatic evaluation of soybean seed traits using RGB

image data and a Python algorithm. Plants, 12(17): 3078.

13. JIN B, C ZHANG, L JIA, Q TANG, L GAO, G ZHAO AND H QI

(2022). Identification of rice seed varieties based on nearinfrared hyperspectral imaging technology combined with deep

learning. ACS Omega, 7(6): 4735-4749.

14. AZNAN A, C GONZALEZ VIEJO, A PANG AND S FUENTES

(2021). Computer vision and machine learning analysis of

commercial rice grains: a potential digital approach for

consumer perception studies. Sensors, 21(19): 6354.

15. JEYARAJ PR, SP ASOKAN AND ERS NADAR (2022).

Computer-assisted real-time rice variety learning using deep

learning network. Rice Science, 29(5): 489-498.

16. WHAN AP, AB SMITH, CR CAVANAGH, JP RAL, LM SHAW,

CA HOWITT AND L BISCHOF (2014). GrainScan: a low-cost,

fast method for grain size and colour measurements. Plant

Methods, 10(1): 23.

17. WIJAYANTO AK, A JUNAEDI, AA SUJASWARA, MBR

KHAMID, LB PRASETYO, C HONGO AND H KUZE (2023).

Machine learning for precise rice variety classification in

tropical environments using UAV-based multispectral sensing.

Agri. Engineering, 5(4): 2000-2019.

18. LI Z, X JIANG, X JIA, X DUAN, Y WANG AND J MU (2022).

Classification method of significant rice pests based on deep

learning. Agronomy, 12(9): 2096.

19. HUANG M, C HE, Q ZHU AND J QIN (2016). Maize seed

variety classification using the integration of spectral and image

features combined with feature transformation based on

hyperspectral imaging. Applied Sciences, 6(6): 183.

20. ZHAO Y, S ZHU, C ZHANG, X FENG, L FENG AND Y HE

(2018). Application of hyperspectral imaging and

chemometrics for variety classification of maize seeds. RSC

Advances, 8: 1337-1345.

21. WANG QJ, SY ZHANG, SF DONG, GC ZHANG, J YANG, R LI

AND HQ WANG (2020). Pest24: a large-scale very small

object data set of agricultural pests for multi-target detection.

Computers and Electronics in Agriculture, 175: 105585.

22. KUMAR R, A GUPTA, S SRIVASTAVA, G DEVI, VK SINGH,

SK GOSWAMI (2020). Diagnosis and detection of seed-borne

fungal phytopathogens. In: KUMAR R AND A GUPTA (Eds.),

Seed-Borne Diseases of Agricultural Crops: Detection,

Diagnosis & Management. Singapore: Springer Nature Pte

Ltd, 107-142.

23. BALA A (2020). Non-parasitic seed disorders of major

agricultural crops. In: KUMAR R AND A GUPTA (Eds.), SeedBorne Diseases of Agricultural Crops: Detection, Diagnosis &

Management. Singapore: Springer Nature Pte Ltd, 809-820.

24. MARTINELLI F, R SCALENGHE, S DAVINO, S PANNO, G

SCUDERI, P RUISI (2015). Advanced methods of plant

disease detection: a review. Agronomy for Sustainable

Development, 35: 1-25.

25. WHETTON RL, KL HASSALL, TW WAINE AND AM

MOUAZEN (2018). Hyperspectral measurements of yellow

rust and fusarium head blight in cereal crops: Part 1:

Laboratory study. Biosystems Engineering, 166: 101-115.

26. GOWEN AA, CP O’DONNELL, PJ CULLEN, G DOWNEY AND

JM FRIAS (2007). Hyperspectral imaging-an emerging

process analytical tool for food quality and safety control.

Trends in Food Science & Technology, 18(12): 590-598.

27. MAHESH S, DS JAYAS, J PALIWAL AND NDG WHITE (2015).

Hyperspectral imaging to classify and monitor quality of

agricultural materials. Journal of Stored Products Research,

61: 17-26.

28. YAN K, MKC SHISHER AND Y SUN (2023). A transfer

learning-based deep convolutional neural network for detection

of Fusarium wilt in banana crops. Agri. Engineering, 5(4): 2381-

2394.

29. YIN J, LI W, J SHEN, C ZHOU, S LI AND J SUO (2025). A

diffusion-based detection model for accurate soybean disease

identification in smart agricultural environments. Plants, 14(5):

675.

30. XU P, L FU, K XU, WB SUN, Q TAN AND Y ZHANG (2023).

Investigation into maize seed disease identification based on

deep learning and multi-source spectral information fusion

techniques. Journal of Food Composition and Analysis, 119:

105254.

31. QIU R, C YANG, A MOGHIMI, M ZHANG, BJ STEFFENSON

AND CD HIRSCH (2019). Detection of Fusarium head blight

in wheat using a deep neural network and color imaging.

Remote Sensing, 11(22): 2658.

32. SASTRY DVSR, HD UPADHYAYA AND CLL GOWD (2014).

Determination of physical properties of chickpea seeds and

their relevance in germplasm collections. Indian Journal of

Plant Genetic Resources, 27(1): 1-9.

33. KADIR MFA, NAN YUSRI, M RIZON, ARB MAMAT, AA JAMAL

AND M MAKHTAR (2015). Automatic mango detection using

texture analysis and randomised Hough transform. Applied

Mathematical Sciences, 9: 6427-6436.

34. GONZALEZ R AND R WOODS (2017). Digital Image

Processing, 4th ed. Pearson International, pp. 464-540.

35. SAFADI T, M KANG, ICC LEITE AND B VIDAKOVIC (2016).

Wavelet-based spectral descriptors for detection of damage

in sunflower seeds. International Journal of Wavelets,

Multiresolution and Information Processing, 14: 1650027.

36. GIRI A, V SAGAN, H ALIFU, A MAIWULANJIANG, S SARKAR,

B ROY AND FB FRITSCHI (2024). A wavelet decomposition

method for estimating soybean seed composition with

hyperspectral data. Remote Sensing, 16(23): 4594.

37. SUREKHA R, R SHOBARANI AND GVS GEORGE (2019).

Seed classification using multi-feature extraction. International

Journal of Innovative Technology and Exploring Engineering,

8(8S): 635-639.

38. CINAR I AND M KOKLU (2019). Classification of rice varieties

using artificial intelligence methods. International Journal of

Intelligent Systems and Applications in Engineering.

39. AGARWAL D, SWETA AND B PATTIMA (2023). Machine

learning approach for the classification of wheat grains. Smart

Agricultural Technology, 3: 100136.

40. SAHA D AND A MANICKAVASAGAN (2022). Chickpea varietal

classification using deep convolutional neural networks with

transfer learning. Journal of Food Process Engineering, 45(3):

e13975.

41. YANG S, L ZHENG, P HE (2021). High-throughput soybean

seeds phenotyping with convolutional neural networks and

transfer learning. Plant Methods, 17: 50.

42. QIU G, E LU, H LU, S XU, F ZENG AND Q SHUI (2018).

Single-kernel FT-NIR spectroscopy for detecting supersweet

corn (Zea mays L. saccharata Sturt) seed viability with

multivariate data analysis. Sensors, 18(4): 1010.

43. LEE HS, YA JEON, YY LEE, GA LEE, S RAVEENDAR AND

KH MA (2017). Large-scale screening of intact tomato seeds

for viability using near infrared reflectance spectroscopy

(NIRS). Sustainability, 9: 618.

44. KANDPAL LM, S LOHUMI, MS KIM, JS KANG AND BK CHO

(2016). Near-infrared hyperspectral imaging system coupled

with multivariate methods to predict viability and vigor in

muskmelon seeds. Sensors and Actuators B: Chemical, 229:

534-544.

45. JOOSEN RVL, J KODDE, LAJ WILLEMS, W LIGTERINK,

LHW VAN DER PLAS AND HWM HILHORST (2010).

GERMINATOR: a software package for high-throughput

scoring and curve fitting of Arabidopsis seed germination. Plant

Journal, 62(1): 148-159.

46. HE X, X FENG, D SUN, F LIU, Y BAO AND Y HE (2019).

Rapid and nondestructive measurement of rice seed vitality

of different years using near-infrared hyperspectral imaging.

Molecules, 24(12): 2227.

47. OLESEN MH, P NIKNESHAN, A SHRESTHA AND B BOELT

(2011a). Viability and germination of castor seeds (Ricinus

communis L.) determined by multispectral imaging. Seed

Science and Technology, 39(2): 389-400.

48. WANG Y AND S SONG (2024). Detection of sweet corn seed

viability based on hyperspectral imaging combined with firefly

algorithm optimized deep learning. Frontiers in Plant Science,

15: Article 1361309.

49. WAKHOLI C, LM KANDPAL, H LEE, H BAE, E PARK, MS

KIM, C MO, WH LEE AND BK CHO (2018). Rapid assessment

of corn seed viability using short wave infrared line-scan

hyperspectral imaging and chemometrics. Sensors and

Actuators B: Chemical, 255: 498-507.

50. SINGH K, H DUDDU, S VAIL, I PARKIN AND S SHIRTLIFFE

(2021). UAV-based hyperspectral imaging technique to

estimate canola (Brassica napus L.) seedpods maturity.

Canadian Journal of Remote Sensing, 47(1): 1-20.

51. FENG Y, X ZHAO, R TIAN, C LIANG, J LIU AND X FAN (2024).

Research on an intelligent seed-sorting method and sorter

based on machine vision and lightweight YOLOv5n. Agronomy,

14(9): 1953.

52. SINGH SK, R JHA, S PANDEY, C MOHAN, CHETNA, S

GHOSH, SK SINGH, S KUMARI AND A SINGH (2025).

Artificial intelligence-based tools for next-generation seed

quality analysis. Crop Design, 4(1): 100094.

53. LI C, H LI, L LIAO, Z LIU AND Y DONG (2023). Real-time

seed sorting system via 2D information entropy-based CNN

pruning and TensorRt acceleration. IET Image Processing,

17(6): 1694-1708.

54. KOPPAD D, KV SUMA AND N NAGARAJAPPA (2024).

Automated seed classification using state-of-the-art

techniques. SN Computer Science, 5(5): 511.

55. GHAMARI S, AM BORGHEI, H RABBANI, J KHAZAEI AND

F BASATI (2010). Modeling the terminal velocity of agricultural

seeds with artificial neural networks. African Journal of

Agricultural Research, 5(5): 389-398.

56. JIAN F (2022). A general model to predict germination and

safe storage time of crop seeds. Journal of Stored Products

Research, 99: 102041.

57. SANKARAN S, A MISHRA, R EHSANI AND C DAVIS (2016).

Separation of viable and non-viable tomato (Solanum

lycopersicum L.) seeds using single seed near-infrared

spectroscopy. Computers and Electronics in Agriculture, 127:

633-640.

58. ZHANG Y, Y WANG AND X LI (2024). Nondestructive detection

of sunflower seed vigor and moisture content based on

hyperspectral imaging and chemometrics. Foods, 13(9): 1320.

59. MAHLEIN AK (2016). Plant disease detection by imaging

sensors - Parallels and specific demands for precision

agriculture and plant phenotyping. Plant Disease, 100(2): 241-

251.

60. SINGH P, A NAYYAR, S SINGH AND A KAUR (2020).

Classification of wheat seeds using image processing and

fuzzy clustered random forest. International Journal of

Agricultural Resources, Governance and Ecology, 16(2): 123.

61. FUENTES A, S YOON, SC KIM AND DS PARK (2017). A

robust deep-learning-based detector for real-time tomato plant

diseases and pests recognition. Sensors, 17(9): 2022.

62. KNIBBE WJ, L AFMAN, S BOERSMA, MJ BOGAARDT, J

EVERS, F VAN EVERT AND A DE WIT (2022). Digital twins

in the green life sciences. NJAS Impact in Agricultural and

Life Sciences, 94(1): 249-279.

63. CARBONELL IM (2016). The ethics of big data in big

agriculture. Internet Policy Review, 5(1).

64. KAMILARIS A AND FX PRENAFETA-BOLDÚ (2018). Deep

learning in agriculture: A survey. Computers and Electronics

in Agriculture, 147(4): 70-90.

65. STAHL BC (2021). Artificial intelligence for a better future: An

ecosystem perspective on the ethics of AI and emerging digital

technologies. Springer Nature, p. 124.

66. KLERKX L, E JAKKU AND P LABARTHE (2019). A review of

social science on digital agriculture, smart farming and

agriculture 4.0: New contributions and a future research

agenda. NJAS - Wageningen Journal of Life Sciences, 90-

91: 100315.

67. SHIMELES A, A VERDIER-CHOUCHANE AND A BOLY

(2018). Introduction: Understanding the challenges of the

agricultural sector in Sub-Saharan Africa. In: Building a

Resilient and Sustainable Agriculture in Sub-Saharan Africa.

Palgrave Macmillan, Cham.

68. POWELL AA (2006). Seed vigour and its assessment. In:

Handbook of Seed Science and Technology. Food Products

Press, pp. 603-648.

69. AOSA (2019). Rules for Testing Seeds. Association of Official

Seed Analysts, USA.

70. U.S DEPARTMENT OF AGRICULTURE - AGRICULTURAL

MARKETING SERVICE (2023). Seed Regulatory and Testing

Division.

71. FOOD AND AGRICULTURE ORGANIZATION OF THE

UNITED NATIONS (2021). Guidelines for the establishment

and management of seed testing laboratories. Joint FAO and

ISTA Handbook.

72. SRINIVASAIAH R, M MEENAKSHI, R CHENNEGOWDA AND

S JANKATTI (2023). Analysis and prediction of seed quality

using machine learning. International Journal of Electrical and

Computer Engineering, 13: 5770.

73. RIJSWIJK K, L KLERKX, M BACCO, F BARTOLINI, E

BULTEN, L DEBRUYNE, J DESSEIN, I SCOTTI AND G

BRUNORI (2021). Digital transformation of agriculture and

rural areas: A socio-cyber-physical system framework to

support responsibilisation. Journal of Rural Studies, 85: 79-

90.

74. INTERNATIONAL SEED TESTING ASSOCIATION (ISTA)

(2023). International Rules for Seed Testing: 2023 Edition.

ISTA.

75. DIBBERN T, LAS ROMANI AND SMFS MASSRUHÁ (2024).

Main drivers and barriers to the adoption of digital agriculture

technologies. Smart Agricultural Technology, 8: 100459.

Submitted

2025-12-31

Published

2025-12-31

How to Cite

LAKSHMIPRASANNA KATA, APARNA KUNA, PALLAVI MANDALAPU, BHARATHI YERAS, SUJATHA PATTA, & ZUBEDA SOHAN. (2025). The New Lens on Seed Testing: AI, ML and Image Analysis. Seed Research, 53(2), 140-147. https://doi.org/10.56093/sr.v53i2.7