Seed Viability, Imbibition Dynamics and Storability of Aegle marmelos (L.) Corrêa: Implications for Propagation and Conservation
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Authors
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MANISH KUMAR VIJAY
ICFRE- Tropical Forest Research Institute, Jabalpur, Madhya Pradesh- 482021, India
Author
Keywords:
Seed viability, tetrazolium, water imbibition, seed dormancy, seed storability, conservationAbstract
The present investigation assessed seed viability, water imbibition behavior, pre-treatment
requirements, and storability of Aegle marmelos (L.) Corrêa. Seed viability, determined through the Tetrazolium
(TZ) test, showed significant variation depending on the TZ concentration and staining duration. The highest
viability (100%) was observed using 0.5% TZ solution after 4 hours of staining, establishing this combination as
optimal for viability assessment. Imbibition studies revealed a progressive increase in seed fresh weight, following
a characteristic triphasic pattern indicative of physiological readiness for germination. Germination experiments
confirmed the absence of dormancy in A. marmelos seeds, indicating that no pre-treatment is necessary for
successful germination. Storage behavior studies demonstrated that seeds dried to a moisture content of 4.58%
and stored at –20°C retained viability above 86% after three months, confirming orthodox storage behavior. This
finding contrasts with earlier reports suggesting intermediate seed storage characteristics. Overall, Aegle marmelos
seeds exhibit high viability, favorable imbibition dynamics, and excellent storability, supporting their effective
utilization in propagation and long-term conservation efforts.
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