Prevalence of vibrio spp. with special reference to Vibrio parahaemolyticus in farmed penaeid shrimp Penaeus vannamei (Boone, 1931) from selected districts of Tamil Nadu, India


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Authors

  • R. Ananda Raja ICAR-Central Institute of Brackishwater Aquaculture (ICAR)
  • R. Sridhar Madras Veterinary College, Chennai-600 007
  • C. Balachandran Madras Veterinary College, Chennai-600 007
  • A. Palanisamy Madras Veterinary College, Chennai-600 007
  • S. Ramesh Laboratory Animal Medicine, Centre for Animal Health Studies, TANUVAS
  • K. Nagarajan Madras Veterinary College, Chennai-600 007

https://doi.org/10.21077/ijf.2017.64.3.69011-18

Abstract

Prevalence of Vibrio spp. with special reference to Vibrio parahaemolyticus (Vp) infection was assessed in Penaeus vannamei farms in Kancheepuram and Thiruvallur districts of Tamil Nadu during the period August 2014 to February 2015. Isolation and identification of bacteria from haemolymph, stomach and hepatopancreas of P. vannamei was done from all the farms for the prevalence of Vp infection. Based on the morphological, physiological and biochemical characterisation, 74 isolates were identified as Vp (35.14%), V. harveyi (21.62%), V. anguillarum (16.22%), V. campbellii (10.81%), V. mimicus (8.11%), V. alginolyticus (5.41%) and Pseudomonas aeruginosa (2.7%). The PCR results showed that there were 26 (35.14%) isolates positive for Vp specific toxR and tlh genes and negative for human pathogenic tdh and trh genes. All isolates were also negative for acute hepatopancreatic necrosis disease (AHPND) specific AP1, AP2, AP3 (pirAvp) and AP4 (pirAvp and pirBvp) genes. The study revealed that there was no AHPND causing Vp strain in farmed P. vannamei from two important coastal districts of Tamil Nadu, India.

Author Biographies

  • R. Ananda Raja, ICAR-Central Institute of Brackishwater Aquaculture (ICAR)
    Aquatic Animal Health and Environment Division
  • R. Sridhar, Madras Veterinary College, Chennai-600 007
    Professor and head, Department of Veterinary Pathology
  • C. Balachandran, Madras Veterinary College, Chennai-600 007
    Dean
  • A. Palanisamy, Madras Veterinary College, Chennai-600 007
    Professor and head, Department of Animal Biotechnology
  • K. Nagarajan, Madras Veterinary College, Chennai-600 007
    Assistant Professor, Department of Veterinary Pathology

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Submitted

2017-03-25

Published

2017-09-28

Issue

Vol. 64 No. 3 (2017)

Section

Note

License

The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.

How to Cite

Raja, R. A., Sridhar, R., Balachandran, C., Palanisamy, A., Ramesh, S., & Nagarajan, K. (2017). Prevalence of vibrio spp. with special reference to Vibrio parahaemolyticus in farmed penaeid shrimp Penaeus vannamei (Boone, 1931) from selected districts of Tamil Nadu, India. Indian Journal of Fisheries, 64(3). https://doi.org/10.21077/ijf.2017.64.3.69011-18