Evaluation of single spore isolates of Agaricus bisporus and their genetic characterization

Authors

  • POOJA KAPOOR*, ABHISHEK KATOCH, NAIYA SHARMA, PREM NATH SHARMA and SUBHASH DHANCHOLIA

Keywords:

Agaricus bisporus, genetic variability, morphological, single spore isolates

Abstract

Single spore isolation is one of the appropriate methods for production of high yielding and quality mushroom strains. In button mushroom (Agaricus bisporus) 500 single spore isolates of U3 and A15 parents were raised for getting their better productivity and quality. Among 500 single spore isolates raised in the present study, 30 (20 from U3 and 10 from A15) were selected on the basis of fast and strandy mycelial growth (>2.0 mm per day) and their colony morphology. Out of 30 tested isolates, U3-1, U3-8 and U3-7 strains were identified as high yielding strains having 22.3, 21.9 and 20.7 kg/100 kg compost respectively as compared to 18.10 kg/100 kg compost in parent U3 strain within six weeks harvesting period. Similarly, isolate A15-5 of parent A-15 was recorded as best yielder strain having 14.95 kg/100kg compost as compared to 9.50 kg/100kg compost obtained by parent A-15 strain within 6 week harvesting period. RAPD studies shown that the percentage of polymorphism detected was 56.9 per cent and maximum number of bands was produced by OPD-12 primer among high yielding single spore isolates. Based on 73 alleles amplified by RAPD markers a dendrogram was generated using NTYSc 2.1 software that clustered high yielding single spore isolates into six variants at 73 per cent similarity coefficient.

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Issue

Vol. 69 No. 3 (2016): Indian Phytopathology

Section

Research Articles

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How to Cite

NAIYA SHARMA, PREM NATH SHARMA and SUBHASH DHANCHOLIA, P. K. A. K. (2016). Evaluation of single spore isolates of Agaricus bisporus and their genetic characterization. Indian Phytopathology, 69(3), 304. http://epubs.icar.org.in/ejournal/index.php/IPPJ/article/view/60152