Morphological and molecular characterization of Trichoderma asperellum strain Ta13

Authors

  • R. THAVA PRAKASA PANDIAN, M. RAJA, A. KUMAR and PRATIBHA SHARMA*

Keywords:

Biocontrol agents, confrontation assay, multi-gene analysis, Trichoderma asperellum

Abstract

Morphological characters are not sufficient to properly identify different species of Trichoderma. Recently, multi-gene phylogeny in combination with morphological characters is used to identify Trichoderma at species level. The current study was focused to characterize Trichoderma asperellum strain Ta13 based on morphology and molecular analysis using genes such as ITS, tef1a, rpb2, act and cal. Light and scanning electron microscopy (SEM) results showed that Ta13 has regularly branched, typically paired conidiophores with straight phialides and globose to sub-globose shaped conidia having size of 2.91µm x 2.37µm with inconspicuous ornamentation. Sequence similarity analysis with reference T. asperellum isolates available in ISTH database showed 99.30, 90.60, 99.20, 98.70, 100 and 99.80 per cent nucleotide similarity for ITS1 and ITS2, tef1a intron4 (large), tef1a intron5 (short), rpb2, cal and act respectively. Confrontation assay clearly showed that Ta13 inhibited several fungal plant pathogens viz. Rhizoctonia solani (88.69 per cent), Fusarium oxysporum f. sp. lycopersici (85.27 per cent), F. moniliforme (84.55 per cent), S. sclerotiorum (80.17 per cent), S. rolfsii (78.26 per cent), and A. brassicicola (77.5 per cent).

Downloads

Issue

Vol. 69 No. 3 (2016): Indian Phytopathology

Section

Research Articles

License

For Authors

As soon as an article is accepted for publication, authors are requested to assign copyright of the article (or to grant exclusive publication and dissemination rights) to the publisher (Indian Phytopathlogical Society). This will ensure the widest possible protection and dissemination of information.

For Readers

While the advice and information in this journal is believed to be true and accurate at the date of its publication, the authors, the editors, nor the publisher can accept any legal responsibility for any errors or omissions that may have been made. The publisher makes no warranty, express or implied, with respect to the material contained herein.

All articles published in this journal are protected by copyright, which covers the exclusive rights to reproduce and distribute the article (e.g., as offprints), as well as all translation rights. No material published in this journal may be reproduced photographically or stored on microfilm, in electronic data bases, on video disks, etc., without first obtaining written permission from the publisher. The use of general descriptive names, trade names, trademarks, etc. in this publication, even if not specifically identified, implies that these names are protected by the relevant laws and regulations.

How to Cite

M. RAJA, A. KUMAR and PRATIBHA SHARMA*, R. T. P. P. (2016). Morphological and molecular characterization of Trichoderma asperellum strain Ta13. Indian Phytopathology, 69(3), 298. http://epubs.icar.org.in/ejournal/index.php/IPPJ/article/view/60166