Phenotypic and genotypic characterization of Phytophthora infestans population of Himachal Pradesh

Authors

  • SANJEEV SHARMA*, B.P. SINGH, SUMIT SHARMA and V.U. PATIL

Keywords:

Late blight, mt DNA haplotype, population structure, SSR fingerprinting

Abstract

Late blight (Phytophthora infestans) is the most devastating disease of potato throughout the world. Monitoring the population structure of P. infestans is essential to devise suitable management strategies. A total of 57 isolates of P. infestans collected from Himachal Pradesh (temperate high hills and low hills) over a period of three years (2008-2010) were characterized using biological and molecular markers. All the isolates were of A2 mating type possessing 9-11 virulence genes and most of them (89.5%) displayed intermediate resistance to metalaxyl. Mitochondrial DNA (mtDNA) haplotyping was done using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results of mitochondrial DNA profiling revealed that all the isolates yielded Ia mtDNA haplotypes which could be a new population. SSR fingerprinting grouped the isolates into seven clusters and microsatellite allele sizes observed in the present study varied from those reported in the literature.

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Published

2016-12-24

Issue

Vol. 69 No. 4 (2016): Indian Phytopathology

Section

Research Articles

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How to Cite

SUMIT SHARMA and V.U. PATIL, S. S. B. S. (2016). Phenotypic and genotypic characterization of Phytophthora infestans population of Himachal Pradesh. Indian Phytopathology, 69(4), 391-395. http://epubs.icar.org.in/ejournal/index.php/IPPJ/article/view/66251