A modification of the Patoèek’s tube test for diagnostic of Synchytrium endobioticum (Schilb.) Perc. a causal agent of potato wart disease

Authors

  • JAROSLAW PRZETAKIEWICZ

Keywords:

Ring test, Diagnostic of S. endobioticum, Resting sporangia, Potato wart disease

Abstract

A modified Patoèek’s tube test (ring test) is a compilation of different techniques. The ring tests has been designed for diagnostic of Synchytrium endobioticum from soil samples with very low density of resting spores of the fungus. Pathotype determination and resistance tests to potato wart disease require fresh galls of the fungus for inoculation of potato genotypes. The ring test has been successfully used to obtain fresh warts from single spores from soil (0.0021 viable resting spore per gram moist soil). Disease incidence increased with inoculum density. The galls were formed from 0.01 spores per g moist soil for all isolates. From 0.2 spores per g moist soil, all tubers had developed fresh warts. The first proliferation of tissue on sprouts was observed from 2 to 12 weeks after inoculation. A threshold sensitivity of the ring test was comparable to PCR-based methods or even higher.

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Issue

Vol. 69 No. 4s (2016): Indian Phytopathology : Special Issue

Section

Research Articles

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How to Cite

PRZETAKIEWICZ, J. (2016). A modification of the Patoèek’s tube test for diagnostic of Synchytrium endobioticum (Schilb.) Perc. a causal agent of potato wart disease. Indian Phytopathology, 69(4s), 35-37. http://epubs.icar.org.in/ejournal/index.php/IPPJ/article/view/71220