An efficient technique for isolation and mass multiplication of Magnaporthe oryzae from blast infected samples

Authors

  • H. RAJASHEKARA*, G. PRAKASH, R.T.P. PANDIAN, S. SARKEL, A. DUBEY, P. SHARMA, V. CHOWDARY, D. MISHRA, T.R. SHARMA and U.D. SINGH

Keywords:

Blast, Magnaporthe, spore drop, lesion print

Abstract

A simple and efficient method has been devised for isolation and single spore culture of Magnaporthe oryzae infecting rice and other graminaceous hosts. Fresh or old blast lesions are incubated in moist chamber set-up for mycelial activation and with spore-drop technique fungal growth observed as Lesion Print (LP) on host extract medium. Through serial dilution plating of macerated colony, monoconidial culture was obtained on rice straw extract agar. For mass multiplication of M. oryzae a combination of rice straw extract and oat meal agar medium enabled quick and profuse sporulation within a short period of 5-7 days at temperature of 25±1°C.

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Issue

Vol. 69 No. 4s (2016): Indian Phytopathology : Special Issue

Section

Research Articles

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How to Cite

P. SHARMA, V. CHOWDARY, D. MISHRA, T.R. SHARMA and U.D. SINGH, H. R. G. P. R. P. S. S. A. D. (2016). An efficient technique for isolation and mass multiplication of Magnaporthe oryzae from blast infected samples. Indian Phytopathology, 69(4s), 68-71. http://epubs.icar.org.in/ejournal/index.php/IPPJ/article/view/71237