Partial characterization of rice Tungro Bacilliform Virus (RTBV) isolate of Odisha, India and analysis of possible recombination of parts of ORF III and ORF IV genes of RTBV

Authors

  • NIVEDITA CHATTOPADHYAY*, RUPSANATAN MANDAL and JAYANTA TARAFDAR

Keywords:

RTBV, Odisha, India isolate, partial sequence, recombination

Abstract

Rice tungro disease is one of the most severe virus diseases of rice (Oryza sativa L.) and is a composite disease caused by joint infection of two unrelated viruses, rice tungro bacilliform virus and rice tungro spherical virus. Extensive survey was conducted in the two major rice-growing states of West Bengal and Odisha to collect the rice plants with tungro-like symptoms. The samples were indexed using PCR-based method with RTBV-specific primers and few of the plant samples of Odisha were positive to RTBV infection. The amplified PCR product of the parts of ORF III and IV genes encoding Odisha RTBV isolate was sequenced and compared with previously published West Bengal isolate and other isolates of Indian and South Asian countries. The Odisha isolate showed close homology with the sequence data of the RTBV Kanyakumari, India isolate which depicted the existence of South Asian isolate of RTBV in Odisha and distantly related to South-East Asian isolates of Philippines, Thailand and Malaysia. Odisha and West Bengal are the neighbouring states. But the previously-reported Chinsurah (acc no. FN377814), West Bengal isolate of RTBV was unrelated to Odisha isolate although both belong to South Asia. The recombination analysis of the partial sequence of the RTBV of Odisha isolate and other South Asian isolates gave the evidence of possible recombination that might have occurred in Malaysian isolate; the Odisha isolate of South Indian type did not show evidence of such recombination.

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Issue

Vol. 69 No. 4s (2016): Indian Phytopathology : Special Issue

Section

Research Articles

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How to Cite

RUPSANATAN MANDAL and JAYANTA TARAFDAR, N. C. (2016). Partial characterization of rice Tungro Bacilliform Virus (RTBV) isolate of Odisha, India and analysis of possible recombination of parts of ORF III and ORF IV genes of RTBV. Indian Phytopathology, 69(4s), 113-115. http://epubs.icar.org.in/ejournal/index.php/IPPJ/article/view/71247