Conventional and q-PCR analysis of Rhizoctonia solani isolates surviving in the rhizospheric soil of wheat and mungbean genotypes under mungbean-wheat cropping system

Authors

  • BHUPENDRA SINGH KHARAYAT*, JAGAT KUMAR, KIRTI RAWAT, SAPNA SHARMA, B.M. BASHYAL, S.C. DUBEY and RASHMI AGGARWAL

Keywords:

q-PCR, Rhizoctonia solani, mungbean, wheat, cropping systems

Abstract

Web blight/wet root rot disease of mungbean caused by Rhizoctonia solani Kühn (Thanatephorus cucumeris Donk) is a ubiquitous soil-borne, plant pathogenic fungus, causes severe loses in agriculturally important food crops and also the major constraint to mungbean crop production in India. Because of its wide host range, saprophytic and facultative parasitic nature and rapid adaptability to wide diversified ecosystem causes often failure of disease management strategies. Pathogenic potential and survival of Rhizoctonia solani isolates in rhizosphere of wheat and mungbean genotypes was evaluated using conventional and Real Time-PCR techniques under glasshouse conditions. Seven isolates of R. solani collected from different locations were evaluated against seven genotypes of wheat in soil inoculation assay under glass house conditions. After harvesting the wheat mungbean was also sown in same pots. Soil sampling were done at 0, 10, 21 days after sowing and after harvesting in both crops. Ning-8139 wheat cultivar found most resistant against six R. solani isolates and RMPM-5 isolate of R. solani recorded most virulent. Maximum concentration of inoculum was observed upto 21 days of sowing. Inoculums started decline after 21 days and it was almost equal to the initial level after the harvest of the wheat crop. Some of the isolates which could not produce disease symptom in wheat were also pathogenic in mungbean.

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Issue

Vol. 69 No. 4s (2016): Indian Phytopathology : Special Issue

Section

Research Articles

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How to Cite

KIRTI RAWAT, SAPNA SHARMA, B.M. BASHYAL, S.C. DUBEY and RASHMI AGGARWAL, B. S. K. J. K. (2016). Conventional and q-PCR analysis of Rhizoctonia solani isolates surviving in the rhizospheric soil of wheat and mungbean genotypes under mungbean-wheat cropping system. Indian Phytopathology, 69(4s), 192-194. http://epubs.icar.org.in/ejournal/index.php/IPPJ/article/view/71276