Evaluation of fungicidal activity of mycogenic silver nanoparticles against Sclerotium rolfsii inciting stem rot disease in groundnut (Arachis hypogaea L.)

Authors

  • Y. CHANDRA SEKHAR, S. KHAYUM AHAMMED, T.N.V.K.V. PRASAD* and R. SARADA JAYALAKSHMI DEVI

Keywords:

Antipathogenic activity, Groundnut rhizosphere, Sclerotium rolfsii, Silver nanoparticles synthesis, Stem rot, Trichoderma sp.

Abstract

Production of functionalized nanoscale particles for agricultural applications is a matter of concern. In the present study, mycogenic silver nanoparticles were synthesized using the fungus Trichoderma viride which was collected from groundnut rhizosphere. The culture filtrate of T. viride isolate was used for the reduction of silver ions (Ag+) in AgNO3 solution to the silver (Ag+) nanoparticles. Different ages (4 days. 6 days, 8 days, 12 days, 15 days and one month) of culture filtrates were screened for the synthesis of silver nanoparticles. The synthesized silver nanoparticles were characterized and among all the treatments the silver nitrate solution treated with 30 days old culture filtrate of T. viride showed the UV absorption peak at 440 nm with maximum intensity (0.911) after 24 h of incubation. The Transmission electron microscopic (TEM) micrographs showed the spherical shaped silver nanoparticles with an average size of 50 nm. The antifungal activity of silver nanoparticles against Sclerotium rolfsii causing stem rot disease in groundnut showed the highest per cent inhibition at 150 ppm concentration (79.44%). In pot culture experiment, no disease was observed in case of groundnut seed treatment with AgNPs and 51.67% and 91.67% PDI was recorded with AgNO3 and control respectively.

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Issue

Vol. 69 No. 4s (2016): Indian Phytopathology : Special Issue

Section

Research Articles

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How to Cite

T.N.V.K.V. PRASAD* and R. SARADA JAYALAKSHMI DEVI, Y. C. S. S. K. A. (2016). Evaluation of fungicidal activity of mycogenic silver nanoparticles against Sclerotium rolfsii inciting stem rot disease in groundnut (Arachis hypogaea L.). Indian Phytopathology, 69(4s), 609-613. http://epubs.icar.org.in/ejournal/index.php/IPPJ/article/view/71405