Marker assisted selection of lines with BLB resistance gene(s) in combination Xa4, Xa7 and Xa21) introgressed in Karma Mahsuri through conventional breeding

Authors

  • AAFREEN KHAN*, C.S. SHUKLA, ANIL S. KOTASTHANE, TOSHY AGARWAL, MOKA SUMA and MEKALA MALLIKARJUN

Keywords:

Bacterial leaf blight, gene pyramid, molecular marker

Abstract

Rice (Oriza sativa L.) is the major food crop of the world. To meet the increasing food demand for the future, concerted efforts are required to increase rice productivity and minimize production losses due to pest and diseases. Bacterial leaf blight, caused by Xanthomonas oryzae pv. oryzae (Xoo) is a serious disease throughout the rice growing countries. The disease, in its severe form, is known to cause losses of 74% to 81%.  Host plant resistance is considered the most economical and ecofriendly strategy for achieving disease resistance and yield stability. The ability to reliably and quickly select desirable material and to eliminate deleterious alleles is critical to the success of a plant breeding program. The use of genetic markers to facilitate the identification (or deleterious) allele in a collection of diverse genotypes is referred to as marker assisted selection (MAS). Karma Mahsuri X IRBB62 derived F2 segregating population was used. Following phenotyping, 40 genotypes each of highly resistant and susceptible were selected. Linked molecular marker (Xa4 Xa7 and Xa21) were used to identify presence and absence of genes in selected genotypes. We report herein homozygous condition for three (Xa4 Xa7 and Xa21) and two (Xa7 and Xa21) in two gene combination in 21 and 19 highly resistant genotypes respectively out of 40. To select appropriate combination of markers for foreground selection and precise placement of gene(s) of interest, high resolution molecular map for Xa4 Xa7 and Xa21 was generated in silico.

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Issue

Vol. 69 No. 4s (2016): Indian Phytopathology : Special Issue

Section

Research Articles

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How to Cite

ANIL S. KOTASTHANE, TOSHY AGARWAL, MOKA SUMA and MEKALA MALLIKARJUN, A. K. C. S. (2016). Marker assisted selection of lines with BLB resistance gene(s) in combination Xa4, Xa7 and Xa21) introgressed in Karma Mahsuri through conventional breeding. Indian Phytopathology, 69(4s), 652-657. http://epubs.icar.org.in/ejournal/index.php/IPPJ/article/view/71416