Biochemical and molecular characterization of sugarcane clones for durable resistance to whip-smut (Ustilago scitaminae Sydow.)

Authors

  • S.V. NALAWADE*, D.V. INDI, R.M. NAIK, S.G. MOHITE and S.M. PAWAR

Keywords:

Sugarcane, Ustilago scitaminea, whip smut

Abstract

Three consistently resistant genotypes viz., Co-6806, Co-6201 and CoM-0265 and four highly susceptible genotypes viz., Co-740, Co-7219, Co-7527 and CoM-08093 to whip-smut under artificial conditions were further evaluated for constitutive and induced defence response. The level of phenols and activities of chitinase, b-1,3-glucanase and PALase were higher in resistant genotypes than the susceptible ones even when not inoculated with the pathogen, suggesting a constitutive in-built defence mechanism. However, these defence responsive enzymes were triggered in response to challenge inoculation with the pathogen more in resistant than susceptible varieties. The smut resistant genotype, Co-6806 recorded higher level induction of b-1,3-glucanase (129.5%) and chitinase (138.0%) with moderate level induction of PALase (45.3%). The genomic DNAs were also evaluated with SSR primers. The primer NKS 11 showed a specific amplicon only in resistant genotypes whereas NKS 49 and NKS 56 showed specific amplified products in susceptible genotypes. These SSR primers could be used to identify whip-smut resistant sugarcane genotypes at early stage which will save time and labour required for the traditional method requiring 12 months.

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Issue

Vol. 69 No. 4s (2016): Indian Phytopathology : Special Issue

Section

Research Articles

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How to Cite

S.G. MOHITE and S.M. PAWAR, S. N. D. I. R. N. (2016). Biochemical and molecular characterization of sugarcane clones for durable resistance to whip-smut (Ustilago scitaminae Sydow.). Indian Phytopathology, 69(4s), 658-662. http://epubs.icar.org.in/ejournal/index.php/IPPJ/article/view/71417