Standardization of methodology for in vivo evaluation of sugarcane clones against wilt

Authors

  • M.L. CHHABRA*, S.K. PANDEY and B. PARAMESWARI

Keywords:

Wilt, Fusarium sacchari, irrigation, stress conditions, root borer, injury, N levels

Abstract

A methodology has been standardized for systematic evaluation of sugarcane clones against wilt caused by Fusarium sacchari in vivo. The various factors influencing the disease development i.e. soil moisture (normal irrigation as well as water stress), time of inoculation, site of inoculation, impact of mechanical injury and nitrogen levels were studied with a set of sugarcane varieties. The pathogen was inoculated with conidial suspension of F. sacchari (104 ml-1) in the first, second and third internodes from the base by plug method of inoculation at three stages i.e. 90, 120 and 150 days after planting. The results were more pronounced in susceptible varieties inoculated at 1st internode during the month of August (150 DAP) under moisture stress conditions. No symptoms were expressed in varieties inoculated either at third internode or at first inoculation stage i.e. 90 DAP. Further, no association of nitrogen levels, mechanical injury or root borer was found on pathogenicity. Of the 13 varieties tested, four viz. Co 419, Co 89003, CoJ 83 and CoS 8436 were found to be susceptible against wilt. The studies concluded that wilt in sugarcane can be induced under field conditions by inoculating the pathogen at 150 DAP with moisture stress conditions.

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Issue

Vol. 69 No. 4s (2016): Indian Phytopathology : Special Issue

Section

Research Articles

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How to Cite

B. PARAMESWARI, M. C. S. P. and. (2016). Standardization of methodology for in vivo evaluation of sugarcane clones against wilt. Indian Phytopathology, 69(4s), 694-698. http://epubs.icar.org.in/ejournal/index.php/IPPJ/article/view/71425