Seed Germination Enhancement of Ziziphus spina-christi (L.) Willd. and Ziziphus mucronata Willd. (Rhamnaceae) Using Different Pre-sowing Treatments in Ethiopia

Mohammed Adefa Seid; Tigist  Wondimu; Asfaw  Degu; Awol  Assefa

doi:10.56093/aaz.v63i4.154240

Authors

Mohammed Adefa Seid Ethiopian Forestry Development (EFD), Addis Ababa, Ethiopia
Tigist Wondimu Department of Plant Biology and Biodiversity Management, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia
Asfaw Degu Department of Plant Biology and Biodiversity Management, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia
Awol Assefa Department of Plant Biology and Biodiversity Management, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia

https://doi.org/10.56093/aaz.v63i4.154240

Keywords:

Dormancy, germination enhancement, Ziziphus spp

Abstract

The stony endocarp and impermeable seed coat of Ziziphus, including fleshy mesocarp inhibit seed germination in both genera. In present study effects of different pre-sowing treatments on seed germination of Z. spina-christi and Z. mucronata was studied. Seeds of each species were collected from the Central and Southern Rift Valley and Northeast Ethiopia. Seeds of each Ziziphus species, after being divided into cracked and uncracked seed-stone groups were subjected to eight different pre-sowing treatments and planted in pots. The mean germination percentage (GP), mean daily germination (GD), mean germination time (GT), and mean germination index (GI), as well as two-way ANOVA and Tukey’s Honestly Significant Difference (THSD), were computed. The two-way ANOVA showed that there exists a significant mean difference among treatment groups under all germination parameters at p<0.05 for both species. The THSD for Z. spina-christi showed that the highest GP was achieved by cracked seed stones soaked in cold water for 48 hours at a room temperature of 25°C (52.7±6), control (48.5±3), soaked in cold water for 24 hours at a room temperature of 25°C (46.5±5.7), and rubbed with sandpaper (41±12.1), which are significantly different at p<0.05, while the highest GI was recorded by cracked seed stones soaked in cold water for 48 hours at a room temperature of 25°C (1.3±0.2), which is significantly different at p<0.05. The THSD for Z. mucronata indicated that the highest GP, along with the highest GI, was achieved by cracked seed stones soaked in 98% H2SO4 for 20 minutes and subsequently rinsed with water (28.5±6), which is significantly different at p<0.05. Overall, the findings of this study will contribute to potential seed enhancement technologies for Ziziphus, with greater implications for the propagation, conservation, and sustainable utilization of the species in the agricultural and pastoral communities of Ethiopia.

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References

AOSA 1983. Association of Official Seed Analysts. Seed Vigor Testing Handbook. 1st edition, AOSA, East Lasing, 88 p.

Bekele-Tesemma, A. 1993. Useful trees and shrubs for Ethiopia: Identification, propagation and management for agricultural and pastoral communities. Technical handbook No 5. Regional Soil Conservation Unit (RSCU) Swedish International Development Authority (SIDA), Embassy of Sweden, Kenya, 486 p.

Bekele-Tesemma, A. 2007. Useful trees of Ethiopia: Identification, propagation and management in 17 agroecological zones. Nairobi: RELMA in ICRAF Project, 552 p.

Benech-Arnold, R.L., Fenner, M. and Edwards, P.J. 1991. Changes in germinability, ABA content and ABA embryonic sensitivity in developing seeds of Sorghum bicolor (L.) Moench. induced by water stress during grain filling. New Phytologist 118: 339-347. https://doi. org/10.1111/j.1469-8137.1991.tb00986.x.

Bewley, J.D. and Black, M. 1994. Seeds. Physiology of development and germination. Plenum, London. 445 p. https://doi.org/10.1007/978-1-4899-1002-8.

El Maaiden, E., El Kharrassi, Y., Qarah, N.A.S., Essamadi, A.K., Moustaid, K. and Nasser, B. 2020. Genus Ziziphus: A comprehensive review on ethnopharmacological, phytochemical and pharmacological properties. Journal of Ethnopharmacology 259: 112950. https://doi. org/10.1016/j.jep.2020.112950.

Ellis, R.H. and Roberts, E.H. 1981. The quantification of ageing and survival in orthodox seeds. Seed Science and Technology 9: 373-409.

El-Siddig, K., Ebert, G. and Lüdders, P. 2001. A comparison of pretreatment methods for scarification and germination of Tamarindus indica L. seeds. Seed Science and Technology 29(1): 271-274.

Gebretsadik, W. 2013. Effect of seed pretreatments on Ziziphus spina-christi germination. Seed Technology 35(1): 145-149. http://www.jstor.org/ stable/24642248.

Gomez, K.A. and Gomez, A.A. 1984. Statistical Procedures for Agricultural Research. 2nd Edition. Wiley & Sons. Canada. 690 p.

Hassen, A., Rethman, N.F.G. and Van Niekerk, W.A. 2005. Effect of different seed treatment options on dormancy breaking, germination and emergence of Ziziphus mucronata (buffalo thorn) seed. Tropical Grassland 39(2): 124-128.

ISTA 1976. International rules for seed testing. Seed Science and Technology 4: 51-177.

ISTA 2005. International Seed Testing Association. International Rules for Seed Testing. Edition 2005. International Seed Testing Association, Bassersdorf, Switzerland.

ISTA 2018. International Seed Testing Association. Chapter 5: The Germination Test. In: International Rules for Seed Testing, International Seed Testing Association: Bassersdorf, Switzerland.

Krishnan, B.M. and Kulasekaran, M. 1984. Studies on seed germination in wild ber (Ziziphus rotundifolia). South Indian Horticulture 32(3): 153- 154.

Maiden, S.K., Jacqueline, A.S. and Vinaya Rai, R.S. 1990. Presowing chemical treatment to hasten germination of Casuarinas equisetifolia. International Tree Crops Journal 6 (2-3): 173-181. https://doi.org/10.1080/01435698.1990.9752882.

Murthy, B.N.S. and Reddy, Y.N. 1989. Effect of different methods on seed germination in ber. Journal of the Maharashtra Agricultural Universities 14(3): 296-298.

Orwa, C., Mutua, A., Kindt, R., Jamnadass, R. and Anthony, S. 2009. Agroforestree Database: a tree reference and selection guide version 4.0. http:// www.worldagroforestry.org/sites/treedbs/ treedatabases.asp.

Oyewole, A.L. and Adedamola, A. 2015. Influence of Temperature Differentials on Germination and Growth of Terminalia ivorensis (A. Chev). Science Research, 3(6): 296-299. https://doi. org/10.11648/j.sr.20150306.15.

Saied, A.S., Gebauer, J. and Buerkert, A. 2008. Effects of different scarification methods on germination of Ziziphus spina-christi seeds. Seed Science and Technology 36(1): 201-205. https://doi. org/10.15258/sst.2008.36.1.22.

Schmidt, L. 2000. Guide to Handling of Tropical and Subtropical Forest Seeds. Humlebaek (Denmark) Danida Forest Seed Centre. 532 p.

Sheoran, V., Kumar, M., Sharma, S.V. and Pathak, D.V. 2018. Effect of seed scarification treatments on ber (Ziziphus rotundifolia Lamk.) seedling biomass. International Journal of Current Microbiology and Applied Sciences 7(12): 2591-2596. https://doi.org/10.20546/ijcmas.2018.712.294.

Steel, R.G.D., Torrie, J.H. and Dickey, D.A. 1997. Principles and procedures of statistics: A biometrical approach, 3rd Ed. New York: McGraw-Hill, 666 p.

Sudhersan, C. and Hussain, J. 2003. In vitro propagation of a multipurpose tree, Ziziphus spina-christi (L.) Willd. Turkish Journal of Botany 27: 167-171.

Vashishtha, B.B and Pareek, O.P. 1979. Flower morphology, fruit set and fruit drop in some Ber (Ziziphus mauritiana Lam.) cultivars. Annals of Arid Zone 18(3): 165-169.

Zietsman, P.C. and Botha, F.C. 1987. Seed germination of Ziziphus mucronata subsp. mucronata. South African Journal of Botany 53(5): 341-344. https:// doi.org/10.1016/S0254-6299(16)31394-1.

Zobel, B. and Talbert, J. 2003. Applied Forest Tree Improvement. The Blackburn Press, 505 p. https://blackburnpress.stores.yahoo.net/ apfotrim.html.