Biofungicidal Potential of Melia azedarach Extracts against Alternaria spp. in Potato: Integrating Phytochemical Profiling with In Vitro and In Vivo Antifungal Efficacy

Fatiha  Ouadah; Amine  Ghelamallah; Ibrahim Elkhalil  Benzohra; Salah Neghmouche  Nacer; Wassima  Lakhdari

doi:10.56093/aaz.v65i1.172456

Authors

Fatiha Ouadah Superior School of Agriculture of Mostaganem (ESAM), Algeria
Amine Ghelamallah Applied Animal Physiology Lab, Abdelhamid Ibn Badis Mostaganem, Mostaganem, Algeria; Department of Agronomy, Faculty of Exact Sciences and Natural and Life Sciences.
Ibrahim Elkhalil Benzohra Experimental Station of Biophysical Environment-Naama, Division of Phoeniciculture, Centre for Scientific and Technical Research on Arid Regions (CRSTRA) Address: Campus Universitaire B.P. 1682 RP, Biskra, Algeria.
Salah Neghmouche Nacer Department of Chemistry, Faculty of Exact Sciences, University of El Oued, P.O. Box 789, El Oued, 39000, Algeria. https://orcid.org/0000-0002-9381-8973
Wassima Lakhdari National Institute of Agronomic Research of Algeria, Station of Sidi Mehdi, Touggourt, Algeria

https://doi.org/10.56093/aaz.v65i1.172456

Keywords:

Melia azedarach; Early Blight; Biocontrol; Botanical Fungicide; Integrated Pest Management (IPM).

Abstract

The development of sustainable alternatives to synthetic fungicides for controlling potato early blight, caused by Alternaria spp., is crucial for food security and environmental health. This study investigates the biofungicidal potential of Melia azedarach L. extracts. The aim was to compare the efficacy and phytochemical basis of methanolic extracts from M. azedarach seeds and leaves against Alternaria spp. The antifungal activity was evaluated in vitro using the poisoned food technique against three Alternaria isolates. The most potent extract was further tested on detached potato leaves (preventive and curative modes) and on whole potato plants under greenhouse conditions. Phytochemical profiling was conducted through TLC, quantification of total phenolics and flavonoids, and UPLC-ESI-MS/MS analysis. The seed extract demonstrated superior and consistent antifungal activity, achieving mycelial growth inhibition (MGI) of 77.8-78.6% in vitro, significantly higher than the leaf extract. In vivo trials confirmed significant disease suppression at the concentration 20%. Preventive application on detached leaves was more effective than curative. UPLC-ESI-MS/MS analysis revealed several major bioactive constituents in the seeds, including oleanolic acid, sinapic acid, oleuropein, and kojic acid. These compounds are known to contribute to membrane destabilization, enzyme inhibition, and the induction of oxidative stress in target pathogens. The seed extract of Melia azedarach demonstrates strong potential as a biofungicide against potato early blight. Its effectiveness appears to arise from a diverse array of bioactive molecules operating through multiple modes of action. Collectively, these results highlight its suitability for incorporation into sustainable potato disease management strategies.

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References

Afifi, M.A. and Sahar, A.Z. 2009. Antifungal effect of neem and some medicinal plant extracts against Alternaria solani, the cause of tomato early blight. Aspects of Applied Biology 96: 251-257.

Akacha, M., Lahbib, K., Daami-Remadi, M. and Boughanmi, N.G. 2016. Antibacterial, antifungal and anti-inflammatory activities of Melia azedarach ethanolic leaf extract. Bangladesh Journal of Pharmacology 11(3): 666-674. https:// doi.org/10.3329/bjp.v11i3.27000.

Aragão, M.Â., Pires, L., Santos-Buelga, C., Barros, L. and Calhelha, R.C. 2024. Revitalising riboflavin: Unveiling its timeless significance in human physiology and health. Foods 13(14): 2255. https://doi.org/10.3390/foods13142255.

Ashfaq, M. and Kayani, S.B. 2025. Fungal endophytes: Applications in agroecosystems and plant protection. In Fungal Endophytes Volume II: Applications in Agroecosystems and Plant Protection (p. 433). Springer. https://doi.org/10.1007/978- 981-97-8804-0.

Bhandari, S., Yadav, P.K. and Sarhan, A. 2021. Botanical fungicides; current status, fungicidal properties and challenges for wide scale adoption: A review. Reviews in Food and Agriculture 2(2): 63-68. http://doi.org/10.26480/ rfna.02.2021.63.68

Carpinella, M.C., Giorda, L.M., Ferrayoli, C.G. and Palacios, S.M. 2003. Antifungal effects of different organic extracts from Melia azedarach L. on phytopathogenic fungi and their isolated active components. Journal of Agricultural and Food Chemistry 51(9) : 2506-2511. https://doi. org/10.1021/jf026083f

De Albuquerque, C.C., Camara, T.R., Mariano, R.D.L.R., Willadino, L., Marcelino Júnior, C. and Ulisses, C. 2006. Antimicrobial action of the essential oil of Lippia gracilis Schauer. Brazilian Archives of Biology and Technology 49 : 527-535. https://doi.org/10.1590/S1516- 89132006000500001.

Deresa, E.M. and Diriba, T.F. 2023. Phytochemicals as alternative fungicides for controlling plant diseases: A comprehensive review of their efficacy, commercial representatives, advantages, challenges for adoption, and possible solutions. Heliyon 9(3) : e13813

DSA (Direction des Services Agricoles) 2022. Statistiques agricoles de la wilaya de Mostaganem. Ministère de l’Agriculture et du Développement Rural.

El Mansouri, K. and Moutaj, R. 2013. Recherche et évaluation de l’activité antifongique des extraits de plantes médicinales [Ph.D. Thesis, Universite Cadi Ayyad].

Gan, C.M., Tang, T., Zhang, Z.Y., Li, M., Zhao, X. Q., Li, S.Y. and Zhou, X. 2025. Unraveling the intricacies of powdery mildew: Insights into colonization, plant defense mechanisms, and future strategies. International Journal of Molecular Sciences 26(8): 3513. https://doi.org/10.3390/ ijms26083513

Hamitouche, N. 2021. Synthèse bibliographique sur la lutte biologique par les huiles essentielles contre la teigne de la pomme de terre Phthorimaea operculella Zeller (Lepidoptera: Gelechiidae) [Doctoral dissertation, Université Mouloud Mammeri].

Jones, J.D. and Dangl, J.L. 2006. The plant immune system. Nature 444(7117): 323-329.

Lakhdari, W., Benyahia, I., Bouhenna, M.M., Bendif, H., Khelafi, H., Bachir, H. and Dehliz, A. 2023. Exploration and evaluation of secondary metabolites from Trichoderma harzianum: GC-MS analysis, phytochemical profiling, antifungal and antioxidant activity assessment. Molecules 28(13) : 5025. https://doi.org/10.3390/ molecules28135025

Lakhdari, W., Bouhenna, M.M., Hamdi, B., Benyahia, I., Bachir, H., Hammi, H. and Dehliz, A. 2024. Phytochemical analysis of bioactive compounds in Pisolithus albus (Cooke & Massee) Priest mushrooms: potential applications in medicine and biotechnology. Natural Product Research 1-11. https://doi.org/10.1080/14786419.2445203

Leiva-Mora, M., Capdesuñer, Y., Villalobos-Olivera, A., Moya-Jiménez, R., Saa, L.R. and Martínez- Montero, M.E. 2024. Uncovering the mechanisms: the role of biotrophic fungi in activating or suppressing plant defense responses. Journal of Fungi 10(9): 635. https://doi.org/10.3390/ jof10090635

Lucas, J.A., Hawkins, N.J. and Fraaije, B.A. 2015. The evolution of fungicide resistance. Advances in Applied Microbiology 90: 29-92. https://doi. org/10.1016/bs.aambs.2014.09.001

Martins, J.M.D.T. 2008. Fungos associados às sementes de faveiro (Dimorphandra mollis Benth.) submetidas a tratamentos pré-germinativos e antifúngicos [Master’s Thesis, Universidade Federal de Lavras].

Meziane, M. and Goumri, H. 2014. The antimicrobial effect of extracts of Melia azedarach on some pathogenic microorganisms. International Journal of Applied Natural Sciences 3: 173-180.

Nakamura, C.V., Ueda-Nakamura, T., Bando, E., Melo, A.F.N., Cortez, D.A.G. and Dias Filho, B.P. 1999. Antibacterial activity of Ocimum gratissimum L. essential oil. Memórias do Instituto Oswaldo Cruz 94: 675-678. https://doi. org/10.1590/S0074-02761999000500022

Negi, P.S., Anandharamakrishnan, C. and Jayaprakasha, G.K. 2003. Antibacterial activity of Aristolochia bracteata root extracts. Journal of Medicinal Food 6(4): 401-403. https://doi. org/10.1089/109662003772519994

Perla, D.E. 2023. Managing Phytophthora diseases of vegetables using host resistance [Ph.D. Thesis, Michigan State University].

Renda, G., Gökkaya, İ. and Şöhretoğlu, D. 2022. Immunomodulatory properties of triterpenes. Phytochemistry Reviews 21(2): 537-563.

Singleton, V.L., Orthofer, R. and Lamuela-Raventós, R.M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology 299: 152-178.

Sofi, T.A., Beig, M.A., Dar, G.H., Ahmad, M., Hamid, A., Ahangar, F., Padder, B.A. and Shah, M.D. 2013. Cultural, morphological, pathogenic and molecular characterization of Alternaria mali associated with Alternaria leaf blotch of apple. African Journal of Biotechnology 12(22): 3306-3314. https://doi.org/10.5897/AJB12.2735

Thakur, M. and Sohal, B.S. 2013. Role of elicitors in inducing resistance in plants against pathogen infection: A review. ISRN Botany 762412. https:// doi.org/10.1155/2013/762412

Tomazoni, E.Z., Pauletti, G.F., da Silva Ribeiro, R.T., Moura, S. and Schwambach, J. 2017. In vitro and in vivo activity of essential oils extracted from Eucalyptus staigeriana, Eucalyptus globulus and Cinnamomum camphora against Alternaria solani Sorauer causing early blight in tomato. Scientia Horticulturae 223: 72-77. https://doi. org/10.1016/j.scienta.2017.04.033

Williamson, E.M. 2001. Synergy and other interactions in phytomedicines. Phytomedicine 8(5): 401-409. https://doi.org/10.1078/0944-7113-00060

Yi, D., Wang, Z. and Peng, M. 2025. Comprehensive review of Perilla frutescens: Chemical composition, pharmacological mechanisms, and industrial applications in food and health products. Foods 14(7): 1252. https://doi.org/10.3390/ foods14071252

Zhishen, J., Mengcheng, T. and Jianming, W. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry 64(4): 555-559. https://doi.org/10.1016/S0308-8146(98)00102-2