In Vitro Propagation of Jojoba [Simmondsia chinensis (Link) Schneider] through Nodal Segments of Female Plants

Meenu Ahuja; Sanjogra Uppal; Anita R Sehrawat; Rachna Yadav; Meenakshi Bhardwaj; Meenakshi Bhardwaj; Vijay Chowdhary

doi:10.56093/aaz.v49i1.63907

Authors

Meenu Ahuja Department of Genetics, CCS Haryana Agricultural University, Hisar 125 004
Sanjogra Uppal Department of Genetics, CCS Haryana Agricultural University, Hisar 125 004
Anita R Sehrawat Department of Genetics, CCS Haryana Agricultural University, Hisar 125 004
Rachna Yadav Department of Genetics, CCS Haryana Agricultural University, Hisar 125 004
Meenakshi Bhardwaj Department of Genetics, CCS Haryana Agricultural University, Hisar 125 004
Meenakshi Bhardwaj Department of Genetics, CCS Haryana Agricultural University, Hisar 125 004
Vijay Chowdhary Department of Biotechnology and Molecular Biology, CCS Haryana Agricultural University, Hisar 125 004,

https://doi.org/10.56093/aaz.v49i1.63907

Abstract

Nodal segments from first, second and third nodes (from the tip of branch) of three mature female plants of jojoba [Simmondsia chinensis (Link) Schneider] were cultured on MS medium supplemented with different concentrations of BAP (1.0 to 5.0 mg L-1). Number of days required for bud break ranged from 7-13 with 5.0 mg L-1 BAP. Increasing BAP concentration from 1.0 to 4.0 mg L-1 , axilliary shoot bud proliferation/multiplication percentage increased. Second and third nodes exhibited better multiple shoot formation (32.75%, 28.0%, respectively) as compared to the first node (15.52%) and the differences were significant. Shoot proliferation was successful at all the concentrations tested with a maximum number of 14 shoots per original explant within 12 weeks at 4.0 mg L-1 BAP and maximum shoot length range (1-3 cm) after 90 days was also observed on same medium. Rooting (42%) of micropropagated shoots was achieved in half MS solid medium containing 2.5 mg L-1 IBA. Key words: Jojoba, female plants, node, BAP, shoot multiplication.