In Vitro Regeneration of Female Plants of Simmondsia chinensis (Link) Schneider (Jojoba) Using Coppic~ Shoots

Abstract

Jojoba -plants are trained during cultivation in our country and the numerous coppice shoots produced during subsequent years are removed periodically. Nodal segments obtained from these coppice shoots of 10 year-old female plants were successfully used for in vitro regeneration. Nodal segments produced axillary shoots on Modified Murashige and Skoog medium (MS-1) within a month. These shoots were incubated in dark in a liquid medium (MS-2) for 72 h (for early root initiation on subsequent medium) and then transferred to rooting medium (MS-3) where root initiation could be observed within a week. More than 80% shoots rooted in a months period.