Rumen Degradability and In Vitro Fermentation Characteristics of Various Cereal Grains
70 / 13
Keywords:
Cereal grains, In vitro gas production, Ruminal degradability, Ruminal fermentationAbstract
A study was conducted to evaluate the in vitro gas production (IVGP), degradability and fermentation characteristics of various cereal grains viz., maize, broken rice, jowar, ragi, wheat, barley and bajra by in vitro gas production technique.The in vitro gas production technique conducted indicated significant (P< 0.01) variation for cereal grains with regard to in vitro gas produced (IVGP) volume, in vitro dry matter degradability (IVDMD %), in vitro organic matter degradability (IVOMD %) and in vitro neutral detergent fibre degradability (IVNDFD %). The IVGP and IVOMD were highest for wheat and barley followed by bajra and lowest for maize, broken rice, jowar and ragi. The order of the IVDMD % from highest to lowest was broken rice> wheat>jowar and maize>bajra>barley>ragi. The IVNDFD (%) was highest for wheat and barley followed by bajra then by ragi and lowest in jowar, broken rice and maize. The partitioning factor was higher (P< 0.01) in broken rice, jowar and maize, followed by ragi, then bajra and was lowest in wheat and barley. The microbial biomass production (MBP) (mg/500mg) was lowest (P< 0.01) in maize, broken rice, jowar and ragi, followed by bajra and highest in wheat and barley. The efficiency of microbial biomass production (EMBP) (g/kg DOM) was higher (P< 0.01) and comparable for broken rice, maize and jowar followed by ragi and then bajra and lowest was from barley and wheat. The ME values from in vitro study varied significantly (P< 0.01) and in descending order was wheat >barley >bajra>broken rice >jowar and maize >ragi. The pH was highest (P> 0.01) in maize and jowar and comparable with broken rice and bajra, followed by ragi and lowest was for wheat and barley. The NH3-N concentration from various cereal grains did not vary, but total volatile fatty acid production was significant. The highest (P< 0.01) TVFA production was from wheat, followed by barley then bajra, jowar and lowest in maize, broken rice and ragi.
References
Amanzougarene, Z, Yuste, S. and Fondevila, M. 2020. Fermentation pattern of several carbohydrate sources incubated in an in vitro semicontinuous system with inocula from ruminants given either forage or concentrate-based diets. Animals. 10:261.
AOAC. 2019. Official Methods of Analysis. 21st ed. Association of Official Analytical Chemists, Maryland, USA.
Blummel, M. and Lebzien, P. 2001. Predicting ruminal microbial efficiencies of dairy ration by in vitro techniques. .Livestock Prod. Sci. 68: 107-117.
Blummel, M., Makkar, H. P. S. and Becker, K. 1997.In vitro gas production: A technique revisited. J.Anim. Physiol and Anim Nutri. 77: 24-34.
Blummel, M., Steingab, H. and Becker, K. 1996.The relationship between in vitro gas production, in vitro microbial biomass yield and 15 N incorporation and its implications for the prediction of voluntary feed intake of roughages.British J. Nutri.77: 911-921.
Duncan, D. B. 1955. Multiple range and multiple F tests. Biometri. 11(1): 1-42.
Giuberti, G., Gallo, A., Masoero, F., Ferraretto, L. F., Hoffman, P. C. and Shaver, R. D. 2014. Factors affecting starch utilization in large animal food production system: A review. Starch-Stärke. 66(1-2):72-90.
Herrera-Saldana ,R. E., Huber, J. T. and Poore, M. H. 1990. Dry matter, crude protein, and starch degradability of five cereal grains. J. Dairy Sci. 73:2386- 2393.
Huhtanen, P. and Sveinbjörnsson J. 2006. Evaluation of methods for estimating starch digestibility and digestion kinetics in ruminants.Ani. feed sci. and technol. 130(1-2):95-113.
Kotarski, S. F., Waniska, R. D. and Thurn, K. K. 1992.Starch hydrolysis by the ruminal microflora. The J.Nutri. 122(1):178-90.
Lanzas, C., Fox, D. G. and Pell, A. N. 2006.Digestion kinetics of dried cereal grains. Ani. feed sci. and technol. 136 : 265–280.
Maize vision. 2022. Federation of Indian Chambers and Commerce of Industry.
McAllister, T. A, Phillippe, R. C., Rode, L. M. and Cheng, K. J. 1993. Effect of the protein matrix on the digestion of cereal grains by ruminal microorganisms. J.anim. Sci.71 (1):205-12.
Menke, K. and Steingass, H. 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim. Res. and Develop.28: 7-55.
Menke, K. H., Raab, L., Salewski, A., Steingass, H., Fritz, D. and Schneider, W. 1979. The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro.The J. Agri. Scie.93(1):217-22.
Momany, F. A., Sessa, D. J., Lawton Gordon, J. W., Sharon, W. S., Hamaker, A. H. and Willett, J. L. 2006 Structural Characterization of α-Zein. J. Agri. Food Chem. 54 (2)543–547.
NDDB. 2012. Nutritive value of commonly available feeds and fodders in India.
Opatpatanakitj, Y., Kellawayj, R. C., Leanj, I. J., Annison, A. G. and Kirby, A. 1994. Microbial fermentation of cereal grains in vitro. Australian. J. Agri. Res. 45: 1247-63.
Rooney, L. W. and Pflugfelder, R. L. 1986. Factors affecting starch digestibility with special emphasis on sorghum and corn. J. Anim. Sci. 63:1607- 23.
Rosendo, O., Freitez, L. and Lopez, R. 2013.Ruminal degradability and summative models evaluation for total digestible nutrients prediction of some forages and by products in Goats. ISRN Vet. Scie., Volume 2013, Article ID 532528, 8 pages.
Snedecor, G.W. and Cochran, W. G.. 1994. Statistical methods, 8th ed. IOWA.
Van Soest, P. J., Robertson, J. B. and Lewis, B. A. 1991.Methods for dietary fibre, neutral detergent fibre, and non starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74(1): 3583-3597.
Yang, S. J, Kim, H. B., Moon, J. B., Kim, N. E., Park, B. K., Lee, S. Y. and Seo, J. 2018. Nutritional evaluation of total mixed rations containing rice grain in an in vitro rumen fermentation system. Korean J.Agri. Sci. 45(4).
Yang, S., Kim, B., Kim, H., Moon, J., Yoo, D., Baek, Y. C., Lee, S. and Seo, J. 2020. Replacement of corn with rice grains did not alter growth performance and rumen fermentation in growing Hanwoo steers. Asian-Australian J. Anim. Sci. 33(2):230-235.
Yoo, D., Hamid, M. M. A, Kim, H., Moon, J., Song, J., Lee, S. and Seo, J. 2020. Substitution effects of rice for corn grain in total mixed ration on rumen fermentation characteristics and microbial community in vitro. J. Anim. Sci. and Technol. 62(5): 638-647
Downloads
Submitted
Published
Issue
Section
License
Copyright remains with the society and author jointly. However, material can be used for research, teaching and to achieve goals of the society.