Impact of Type and Level of Herbs Supplemented to Total Mixed Ration on the Fermentation Pattern and In vitro Methane Emission
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Authors
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M.P.S. Bakshi
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A.S. Singh
Guru Angad Dev Veterinary and Animal Science University, Ludhiana-141004,
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M. Wadhwa
Guru Angad Dev Veterinary and Animal Science University, Ludhiana-141004,
Abstract
This study was taken up to assess the effect of dry extract of six herbal feed additives [HFAs; Kulthi (Dohichos biflorus), patha (Cissampelos pareria), aritha (Sapindus trifoliatus), methi (Trigonella foenum graecum), shikakai (Acacia concina) and shatavari (Asparagus racemosus)] rich in saponins supplemented at 1-4% of total mixed rations (TMR) on DM basis on the in vitro fermentation pattern and methane emission. A TMR with roughage to concentrate (R:C) ratio of 70:30 on DM basis was formulated. The data was analyzed by 2 x 6 x 5 factorial design. The net gas production (NGP), digestibility of NDF, OM, ME, total and individual volatile fatty acids (VFAs) were higher (P<0.01) at 24h incubation in comparison to that observed at t½. The NGP and ME in the TMR supplemented with shatavari was higher (P<0.01) than all other HFAs supplemented groups and increased (P<0.01) with the increase in the level of HFAs. The total and individual VFAs were the highest (P<0.01) when the diet was supplemented with HFAs at 3% levels. The methane production was lowest (P<0.01) and the fermentation efficiency was the highest in TMR supplemented with shatavari. The methane emission was depressed at all levels of HFAs as compared to control group. The methane emission at t-half was observed to be the lowest in the TMR supplemented with patha, kulthi or shatavari at 2% on DM basis. It was concluded that best response in above parameters could be ascertained in TMR supplemented with shatavari @ 2-3% on DM basis.
References
Agarwal, N., Kamra, D.N., Chaudhary, L.C. and Patra, A.K. 2006. Effect of Sapindus mukorossi extracts on in vitro methanogenesis and fermentation characteristics in buffalo rumen liquor. Journal of Applied Animal Research. 30: 1-4.
AOAC. 2007. Official Methods of Analysis, 18th Edn. Association of Official Analytical Chemists, Gaithersburg, Maryland, USA.
Bakshi, M.P.S. and Wadhwa, M. 2010. Significance of plant secondary metabolites in relation to methane production by ruminants. In: Animal Nutrition: Strategies for Environment Protection and Poverty Alleviation (Eds. Pattanaik, A.K., Verma, A.K. and Dutta, N.), Volume I. Proceedings of VII Biennial Animal Nutrition Association Conference, December 17-19, 2010, Bhubaneswar, pp 25-28.
Blummel, M. and Lebzien, P. 2001. Predicting ruminal microbial efficiencies of dairy rations by in vitro techniques. Livestock Production Science. 68: 107-117.
Cottyn, B.G. and Boucque, C.V. 1968. Rapid methods for the gas chromatographic determination of volatile acids in rumen fluid. Journal of Agricultural and Food Chemistry.16: 105-107.
Deuri, P., Sood, N., Wadhwa, M. and Bakshi, M.P.S. 2019. Screening tree leaves for bioactive compounds and their effect on in vitro fermentation and methane production from total mixed ration. Animal Nutrition and Feed Technology. 19: 241-253.
Feng, Z.H., Cao, Y.F., Gao, Y.X., Li, Q.F. and Li, J.G. 2012. Effect of gross saponins of Tribulus terrestris on ruminal fermentation and methane production in vitro. Journal of Animal Veterinary advances.11: 2121-2125.
Goel, G., Makkar, H.P.S. and Becker, K. 2008. Effects of Sesbania sesban and Carduuspy cnocephalus leaves and fenugreek (Trigonella foenum-graecum L.) seeds and their extracts on partitioning of nutrients from roughage- and concentrate-based feeds to methane. Animal Feed Science and Technology. 147: 72-89.
Hristov, A.N., McAllister, T.A., Van Herk, F.H., Cheng, K-J., Newbold, C.J. and Cheeke, P.R. 1999. Effect of Yucca schidigeraon ruminal fermentation and nutrient digestion in heifers. Journal of Animal Sciences. 77: 2554-2563.
Hundal, J.S., Wadhwa, M. and Bakshi, M.P.S. 2020. Effect of herbal feed additives containing saponins on rumen fermentation pattern. Indian Journal of Animal Science. 90: 237-243.
Jadhav, R.V., Kannan, A., Bhar, R., Sharma, O.P., Gulati, A., Rajkumar, K., Mal, G., Singh, B. and Verma, M.R. 2018. Effect of tea (Camellia sinensis) seed saponins on in vitro rumen fermentation, methane production and true digestibility at different forage to concentrate ratios. Journal of Applied Animal Research. 46: 118-124.
Karásková, K., Suchý, P. and Straková, E., 2015. Current use of phytogenic feed additives in animal nutrition: A review. Czech Journal of Animal Science. 60: 521-530.
Lila, Z.A., Mohammed, N., Kanda, S., Kamada, T. and Itabashi, H. 2003. Effect of sarsaponin on rumen fermentation with particular reference to methane production in vitro.Journal of DairyScience. 86: 3030-3036.
Lila, Z.A., Mohammed, N., Kanda S., Mitsunori, K., and Itabashi, H. 2005. Saponin effects on ruminal fermentation and microbes, methane production, digestibility and blood metabolites in steers. Asian Australasian Journal of Animal Sciences. 18: 1746-1751.
Menke, K.H. and Steingass, H. 1988. Estimation the energetic feed value obtained by chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development. 28: 7-55.
Menke, K.H., Raab, L., Salweski, A., Steingass, H., Fritz, D. and Scheider, W. 1979. The estimation of digestibility and metabolizable energy content of ruminant feedstuffs from the gas production when they are incubated with rumen liquor in vitro. Journal of Agricultural Science. Cambridge, 93: 217-222.
Miguel, M.G., 2010. Antioxidant and anti-inflammatory activities of essential oils: A short review. Molecules. 15: 9252-9287.
Miller, J.R. 2003. Graph Pad Prism Version 4.00. Step-by-step examples, Graph Pad Software Inc., San Diego, CA, USA.
OECD-FAO. 2021. Agricultural Outlook 2021-2030.Executive summary. Organisation for Economic Co-operation and Development (OECD) and the Food and Agriculture Organization (FAO) of the United Nations. www.fao.org › publications ›
Singh, A.S. 2017. Effect of supplementing saponin containing herbs to total mixed rations on the performance of growing male buffaloes. MVSc Thesis, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana -141004, India.
Snedecor, G.W. and Crochan, W.G. 1994. Statistical Methods.7th Edn. Oxford and IBH Publications, New Delhi.
SPSS. 2009. Statistical Packages for Social Sciences, Version 16. SPSS Inc., Chicago, Illinois, USA.
UNEP and CCAC. 2021. Global methane assessment: benefits and costs of mitigating methane emissions. Nairobi. United Nations Environment Programme (UNEP) and Climate and Clean Air Coalition (CCAC).Accessed on 11th June, 2022.
Unnawong, N., Cherdthong, A. and So, S. 2021. Crude saponin extract from Sesbania grandiflora (L.) Pers pod meal could modulate ruminal fermentation, and protein utilization, as well as mitigate methane production.Tropical Animal Health and Production. 53. doi:10.1007/s11250-021-02644-z
Van Soest, P.J., Robertson, J.B., Lewis, B.A.1991. Methods for dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science. 74: 3583-3597.
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