Silkworm Pupae Meal: A Potential Unconventional Protein Source for Animal Feeding
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Keywords:
:Livestock, Poultry,Protein source,Silkworm pupae meal, Unconventional feed ingredientAbstract
ABSTRACT:
Silkworm pupae are thrown away as a waste by-product of silk industry after reeling silk from the cocoons. The huge amount of the pupae produced/disposed (about 5500 pupae per kg of raw silk), pose a severe pollution threat to environment, soil and water. This demands technological interventions for efficient disposal or utilization of these pupae in a productive enterprise. Considering the fact that the pupae represent an active growth stage of an insect, they offer a potential nutrient-rich alternative for livestock feeding. Off-late, the silkworm pupae meal (SWPM) has been acknowledged as top-class protein source of animal origin that finds way in utilization as a low-cost unconventional feeding resource for livestock and poultry. It has a high nutritive value and acts as a decent source of protein, carbohydrates, fat, fiber, ash, methionine, lysine, calcium and phosphorus, and could offer a cheap animal food for the growing livestock sector which is generally regarded as resource-hungry in terms of insufficient availability of quality feed and fodder. The increasing dependence of expanding mankind on animal proteins, in decreasing availability of plant-based proteins, further necessitates the development of strategies and policies for harnessing the industrial by-products as alternate animal-foods and SWP finds its best use as an alternate, economical and efficient animal food.
References
Al-Qazzaz, M.F. and Ismail, D.B. 2016. Insect meal as a source of protein in animal diet. Animal Nutrition and Feed Technology. 16(3):527-547.
Anonymous, 2021. Central Silk Board,Ministry of Textiles, Government of India.https://www.india.gov.in/official-website-central-silk-board
Anonymous,2022. International Sericulture Commission. https://inserco.org/en/statistics
Bhagat, R.P. and Barat, S. 2016. Impact of artificial feed on survival and growth of rainbow trout, Oncorhynchusmykiss (Walbaum) during exogenous feeding inracewaysof Kathmandu, Nepal. Internationaljournal of pure and applied bioscience. 4(2): 9-16.
Bhuiyan, A.K.M.A., Begum, N.N., Begum, M. and Hoq, M.E. 1989. Survey of potential fish feed ingredients of Bangladesh based on their availability and biochemical composition. Fisheries Research Institute Research Project Report. Fisheries Research Institute, Bangladesh.
Blair, R. 2018. Nutrition and feeding of organic poultry. CABI.
Bora, L.R. and Sharma, P.K. 1965. Assam Muga Silkworm, AntharacaassamensisWw, pupae as a protein supplement in chick ration. Indian Veterinary Journal. 42:354-359.
Borthakur, S. and Sarma, K. 1998. Effect of some non-conventional fish meal replacers on the growth, feed conversion and body composition of Clarias batrachus (Linn.) fingerlings. Environment and Ecology. 16(3):694-698.
Bose, P.C. and Majumder, S.K. 1990. Biochemical composition of pupae waste and utilization. Indian silk. 29(2):45-46.
Bovera, F., Piccolo, G., Gasco, L., Marono, S., Loponte, R., Vassalotti, G., Mastellone, V.,Lombardi, P., Attia, Y.A. and Nizza, A. 2015. Yellow mealworm larvae (TenebriomolitorL.) as a possible alternative to soybean meal in broiler diets. British Poultry Science. 56: 569–575.
Cao, H., Cai, D.F., Yang, P.C., Liu, Z.G. and Xia, L.X. 2017. Expressionand immunological identification of MBP-Bmlp7from Bombyx mori.Journal of Shenzhen University Science and Engineering. 34(2):117–121.
Chandrasekharaiah, M., Sampath, K.T. and Praveen, U.S. 2004. Chemical composition and in vitro digestibility of certain commonly used feedstuffs in ruminant rations. Indian Journal of Dairy Science. 57(2): 114-117.
Chatterjee, A. 2022. Development of Sericulture in Murshidabad with Special Reference to Women’s Participation. In Livelihood Enhancement Through Agriculture, Tourism and Health, Springer, Singapore, p 205-221.
Chopra, A.K., Malik, N.S., Makker, G.S. and Ichhponani, J.S. 1971. Evaluation of poultry feeds available in India. 1. Proximate analysis, energy values and basic amino-acid contents of feed ingredients. Journal of Research-Punjab Agricultural University. 8(2): 232-236.
Coll, J.F.C., Crespi, M.P.A.L., Itagiba, M.G.O.R., Souza, J.C.D., Gomes, A.V.C. andDonatti, F.C.1992. Utilization of silkworm pupae meal (Bombyx mori L) as a source of protein in the diet of growing-finishing pigs. Revista da SociedadeBrasileira de Zootecnia. 21:378-383.
Dar, K.A., GanieAuqib, H., Ganie, N.A., Farhat, S., Sharma, R.K., Salib, S., Omaise, A., Rafique, A.A. and Parviz, S. 2021. Jammu and Kashmir silk industry: Problems and prospects. The Pharma Innovation. 10(6): 369-371.
Dutta, A., Dutta, S. and Kumari, S. 2012. Growth of poultry chicks fed on formulated feed containing silkworm pupae meal as a protein supplement and commercial diet. Online Journal of Animal and Feed Research. 2(3): 303-307.
Elahi, U., Wang, J., Ma, Y.B., Wu, S.G., Wu, J., Qi, G.H. and Zhang, H.J. 2020. Evaluation of yellow mealworm meal as a protein feedstuff in the diet of broiler chicks. Animals. 10(2):224.
Elahi, U., Xu, C.C., Wang, J., Lin, J., Wu, S.G., Zhang, H.J. and Qi, G.H. 2022. Insect meal as a feed ingredient for poultry. Animal Bioscience. 35(2):332.
Fagoone, I. 1983. Possible growth factors for chickens in silkworm pupae meal. British Poultry Science. 24(3): 295-300.
Gugołek, A., Kowalska, D., Strychalski, J., Ognik, K. and Juśkiewicz, J. 2021. The effect of dietary supplementation with silkworm pupae meal on gastrointestinal function, nitrogen retention and blood biochemical parameters in rabbits. BMC Veterinary Research. 17(1): 1-14.
Habib, M.A.B., Hasan, M.R., Akand, A.M. and Siddiqua, A. 1994. Evaluation of silkworm pupae meal as a dietary protein source for Clarias batrachus fingerlings. Aquaculture. 124(1-4): 62.
Habib, M.A.B. and Hasan, M.R. 1995. Evaluation of silkworm pupae as a dietary protein source for Asian catfish Clarias batrachus (L.) fingerling. Bangladesh Journal of Aquaculture. 17:1-7.
Hasan, M.R. 1991. Studies on the use of poultry offal and silk worm pupae as dietary protein sources for Indian major carp, Catla (Hamilton)[Bangladesh]. Part B: Livestock, Fisheries, Agricultural Engineering and Socio-economics.
Hertrampf, J.W. and Piedad-Pascual, F. 2003. Silkworm Pupae Meal. In Handbook on Ingredients for Aquaculture Feeds, Springer, Dordrecht,p 372-378.
Hossain, M.A., Nahar, N. and Kamal, M. 1997. Nutrient digestibility coefficients of some plant and animal proteins for rohu (Labeorohita). Aquaculture. 151(1-4): 37-45.
Hu, W., Liang, Z.L., Wang, L.L. and Liu, Z.G. 2016. Expression, purification,immunological identification and B cell epitope analysisof allergen CPH30 in silkworm (Bombyx mori) pupae. Acta Entomologica Sinica. 59(04): 377–381.
Hu, W., Liang, Z.L., Wang, L.L., Zhong, H.L. and Liu, Z.G. 2017.Expression, purification and bioimformatics analysis of allergen protein profile in from silkworm (Bombyx mori). Immunology Journal of Chinese. 33(01): 81–84.
Hu, B., Li, C., Zhang, Z., Zhao, Q., Zhu, Y.,Su, Z. and Chen, Y.2017. Microwave-assisted extraction of silkworm pupal oil and evaluationof its fatty acid composition, physicochemical properties and antioxidant activities. Food Chemistry. 231: 348–355.
Ioselevich, M., Steinga, H., Rajamurodov, Z. and Drochner, W. 2004. Nutritive value of silkworm pupae for ruminants. In: VDLUFA Kongress, Qualitätssicherung in landwirtschaftlichen Produktionssystemen, Rostock, p 116.
Jeong, K.Y., Son, M., Lee, J.Y., Park, K.H., Lee, J. and Park, J. 2016.Allergenic characterization of 27-kDaglycoprotein, a novel heat stable allergen, from the pupa of Silkworm, Bombyx mori. Journal of Korean Medical Science. 31(1): 18–24.
Ji, W., Wang, Y. and Tang, J.2010. Apparent digestibility coefficients of selected feed ingredients for Japanese seabass (Lateolabrax japonicus) reared in sea water. Journal of Fisheries China. 34:101-107.
Joshi, P.S., Rao, P.V., Mitra, A. and Rao, B.S. 1980. Evaluation of deoiled silkworm pupae-meal on layer performance. Indian Journal of Animal Sciences. 50(11): 979-982.
Karthick Raja, P., Anand, S., Stephen Sampathkumar, J. and Padmavathy, P. 2019. Silkworm pupae meal as alternative source of protein in fish feed. Journal of Entomology and Zoology Studies. 7(4):78-85.
Khan, S.A. and Zubairy, A.W.1971. Chemical composition and nutritive value of Tusser silkworm pupae. Indian Journal of Animal Sciences. 41:1070-1072.
Khan, S.H. 2018. Recent advances in role of insects as alternative protein source in poultry nutrition. Journal of Applied Animal Research. 46(1): 1144-1157.
Khan, S., Khan, R.U. and Ullah, Q. 2020. Does the gradual replacement of spent silkworm (Bombyx mori) pupae affect the performance, blood metabolites and gut functions in White Leghorn laying hens?. Research in Veterinary Science. 13: 574-577.
Khatun, R., Azmal, S.A., Sarker, M.S.K., Rashid, M.A., Hussain, M.A. and Miah, M.Y. 2005. Effect of silkworm pupae on the growth and egg production performance of Rhode Island Red (RIR) pure line. International Journal of Poultry Science. 4(9): 718-720.
Koreleski, J., Smyk, D., Kubicz, M. and Gawlik, Z. 1993. Nutritive value of silkworm pupa meal (Bombyx mori L.). Roczniki Naukowwe Zootechniki. 20: 291-297.
Koundinya, P.R. and Thangavelu, K. 2005. Silk proteins in biomedical research. Indian Silk. 43(11): 5-8.
Kröncke, N., Grebenteuch, S., Keil, C., Demtröder, S., Kroh, L., Thünemann, A.F., Benning, R. and Haase, H. 2019. Effect of different drying methods on nutrient quality of the yellow mealworm (Tenebriomolitor L.). Insects. 10(4): 84.
Lee, J., Choi, I.C., Kim, K.T., Cho, S.H. and Yoo, J.Y. 2012. Response of dietary substitution of fishmeal with various protein sources on growth, body composition and blood chemistry of olive flounder (Paralichthysolivaceus, Temminck& Schlegel, 1846). Fish physiology and biochemistry. 38(3): 735-744.
Liang, Z.L., Li, P., Liu, Z.G. and Liu, X.Y. 2016. Clone, expression, purification,immunological identification and bioinformatics analysis of allergencuticular protein RR-2motif 63 precursor (CPR63) of silkworm(Bombyx mori) pupae. Immunological Journal. 32(7): 565–569.
Liang, Z.L., Liu, X.Y., Liu, Z.G., and Chen, X.X. 2017. Purification,immunological identification and bioinformatics study ofgene: LOC101743840 from Bombyx mori pupae. China Tropical Medicine. 17(7): 641–645.
Lin, S.W., Njaa, L.R., Eggum, B.O. and Shen, H.Y. 1983. Chemical and biological evaluation of silk worm chrysalid protein. Journal of the Science of Food and Agriculture. 34(8): 896-900.
Liu, Z., Xia, L., Wu, Y., Xia, Q., Chen, J. and Roux, K. H. 2009. Identificationand characterization of an arginine kinase as a major allergen from silkworm (Bombyxmori) larvae. International Archives of Allergy and Immunology. 150(1): 8–14.
Longvah, T., Mangthya, K. andRamulu, P.2011. Nutrient composition and protein quality evaluation of eri silkworm (Samiaricinii) prepupae and pupae. Food Chemistry. 128(2):400-403.
Zhi Yi, L. 1997. Insects as food in China. Ecology of Food and Nutrition. 36(2-4): 201-207.
Ma, Y.H., Hu, W., Liang, Z.L., Wang, H. and Liu, Z.G. 2016.Immunological and bioinformatics analysis of a recombinant allergenof silkworm CSP5. Current Immunology. 36(6): 466–470.
Majoankar, S.B. and Biamber, C.V.1987. Utilization of silkworm pupae meal in broiler diets. Sovenior, Institute of poultry management in India,p 72-75.
Majumdar, D. 2002. Effects of varying levels of dietary protein and cost effective formulated diets on growth and survival of Pangasiussutchi (Fowler) (Doctoral dissertation, Kolkata).
Makkar, H.P., Tran, G., Heuzé, V. and Ankers, P. 2014. State-of-the-art on use of insects as animal feed. Animal Feed Science and Technology. 197: 1-33.
Medhi, D., Math, N.C. and Sharma, D.N.2009a. Effect of silkworm pupae meal and enzyme supplementation on blood constituents in pigs. Indian Veterinary Journal. 86:433-434.
Medhi, D., Nath, N.C., Gohain, A.K. andBhuyan, R. 2009b. Effect of silkworm pupae meal on carcass characteristics and composition of meat in pigs. Indian Veterinary Journal. 86:816-818.
Medhi, D.2011. Effects of enzyme supplemented diet on finishing crossbred pigs at different levels of silkworm pupae meal in the diet. Indian Journal of Field Veterinarian. 7(1):24-26.
Mitsuhashi, J. 1997. Insects as traditional foods in Japan. Ecology of Food and Nutrition. 36:187-199.
Nandeesha, M.C., Gangadhara, B., Varghese, T.J. and Keshavanath, P. 2000. Growth response and flesh quality of common carp, Cyprinus carpio fed with high levels of non-defatted silkworm pupae. Asian Fisheries Science. 13(3): 235-242.
Narang, M.P. and Lal, R. 1985. Evaluation of some agro-industrial wastes in the feed of Jersey calves. Agricultural wastes. 13(1): 15-21.
Nisha, S.N., Jothi, B.A. and Geetha, B. 2014. Growth Performance and Haematological Parameters of the Ornamental Fish, Maylandiaestherae, Fed Varying Inclusion of Silkworm Pupae Meal. Advances in Biological Research. 8(6): 268-273.
Olaniyi, C.O. and Babasanmi, G.O. 2013. Performance characteristics of African Cat fish (Clariasgariepinus) fed varying inclusion levels of silk worm pupae (Anaphe infracta). Bangladesh Journal of Animal Science. 42(1): 76-80.
Oso, J.A. and Iwalaye, O.A. 2014. Growth performance and nutrient utilization efficiency of Clariasgariepinus juveniles fed Bombyx mori (mulberry silkworm) meal as a partial replacement for fishmeal. British Journal of Applied Science & Technology. 4(26): 3805.
Panda, B.1970. Processing and utilization of agro-industrial byproducts as livestock and Poultry feed. Indian Poultry Gazette. 55: 38.
Penkov, D., Kipriotis, E., Grekov, D. and Ivanov, K. 2002. Determination of the amino acid content and Protein News. 3:2.
Priyadarshini, P., Maria Joncy, M. and Saratha, A.M. 2017. Industrial utilization of silkworm pupae–a review. Journal of International Academic Research for Multidisciplinary. 5: 2320-5083.
Rafiullah,2016. Replacement of soybean meal with silkworm meal (Bombyx mori) in poultry ration. Ph.D. Thesis In poultry science, University of Agriculture, Peshawar.
Rangacharyulu, P.V., Giri, S.S., Paul, B.N., Yashoda, K.P., Rao, R.J., Mahendrakar, N.S., Mohanty, S.N. and Mukhopadhyay, P.K. 2003. Utilization of fermented silkworm pupae silage in feed for carps. Bioresource technology. 86(1): 29-32.
Rao, P.U. 1994. Chemical composition and nutritional evaluation of spent silkworm pupae. Journal of Agricultural and Food Chemistry. 42(10):2201-2203.
Rashmi, K.M., Chandrasekharaiah, M., Soren, N.M., Prasad, K.S., David, C.G., Thirupathaiah, Y. and Shivaprasad, V. 2018. Effect of dietary incorporation of silkworm pupae meal on in vitro rumen fermentation and digestibility. Indian Journal of Animal Sciences. 88(6): 731-735.
Rashmi, K.M., Chandrasekharaiah, M., Soren, N.M., Prasad, K.S., David, C.G., Thirupathaiah, Y. and Shivaprasad, V. 2022. Defatted silkworm pupae meal as an alternative protein source for cattle. Tropical Animal Health and Production. 54(5): 327.
Roychoudhury, N. and Mishra, R.K. 2020. Silkworm as human food. Van Sangyan. 7(8): 35-39.
Sadat, A., Biswas, T., Cardoso, M.H., Mondal, R., Ghosh, A., Dam, P., Nesa, J., Chakraborty, J., Bhattacharjya, D., Franco, O.L. and Gangopadhyay, D. 2022. Silkworm pupae as a future food with nutritional and medicinal benefits. Current Opinion in Food Science. 44: 100818.
Sahib, Q.S. 2019. Effect of feeding graded levels silkworm pupae meal based calf starter diet on performance of crossbred calves. M.V.Sc. Thesis, Division of Animal Nutrition, Faculty of Veterinary Sciences and Animal Husbandry,Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu and Kashmir, India.
Sahib, Q.S., Ahmed, H.A., Ganai, A.M., Farooq, J., Sheikh, G.G., Sheikh I.U. and Beigh, Y.A. 2023. Evaluation of silkworm pupae meal based calf starter diet on the performance of crossbred cattle calves. Indian Journal of Animal Sciences. 93(9): 903-906.
Sampath, K.T., Chandrasekharaiah, M. and Thulasi, A. 2003. Limiting amino acids in the bypass protein fraction of some commonly used feedstuffs. Indian Journal of Animal Sciences. 73:1155-1158.
Sawhney, S. 2014. Effect of partial substitution of expensive ingredient i.e. fish meal on the growth of (Tor putitora) fed practical diets. Journal of International Academic Research for Multidisciplinary. 2(7):482-489.
Sheikh, I.U., Sapcota, D., Dutta, K.K. and Sarma, S. 2005. Effect of dietary muga silkworm pupae meal on the carcass characteristics of broilers. Indian Veterinary Journal. 82(7): 752-755.
Sheikh, I.U. and Sapcota, D. 2007. Economy of feeding muga silkworm pupae meal in the diet of broiler. Indian Veterinary Journal. 84(7): 722-724.
Sheikh, I.U., Banday, M.T., Baba, I.A., Adil, S., Nissa, S.S., Zaffer, B. and Bulbul, K.H. 2018. Utilization of silkworm pupae meal as an alternative source of protein in the diet of livestock and poultry: A review. Journal of Entomology and Zoology Studies. 6(4): 1010-1016.
Suresh, H.N., Mahalingam, C.A. and Pallavi. 2012. Amount of chitin, chitosan and chitosan based on chitin weight in pure races of multivoltine and bivoltine silkworm pupae Bombyx mori L. International Journal of Science & Nature. 3:214-216.
Takeda, S. 2009. Sericulture. In Encyclopedia of Insects, Academic Press, p 912-914.
Trivedy, K., Kumar, S.N., Mondal, M.and Bhat, C.A. 2008. Protein banding pattern and major amino acid component in de-oiled pupal powder of silkworm, Bombyx mori Linn. Journal of Entomology. 5:10-16.
Tuigong, D.R., Kipkurgat, T.K. and Madara, D.S. 2015. Mulberry and silk production in Kenya. Journal of Textile Science & Engineering. 5(6): 1.
Ullah, R., Khan, S., Hafeez, A., Sultan, A., Khan, N.A., Chand, N. and Naseer, A. 2017. Silkworm (Bombyx mori) meal as alternate protein ingredient in broiler finisher ration. Pakistan Journal of Zoology. 49(4).
Umalatha, H., Gangadhar, B., Hegde, G. and Sridhar, N. 2018. Digestibility of three feed ingredients by Catlacatla (Hamilton, 1822). Oceanography & Fisheries Open Access Journal. 5(5):99-104.
Valerie, H., Tran, G., Giger-Reverdin, S. and Lebas, F. 2015. Silkworm pupae meal. Feedipedia, a programme by INRA, CIRAD, AFZ and FAO.
Vichasilp, C., Wechavitan, P., Poungchompu, O. and Wiwacharn, P. 2016. Extraction of 1-Deoxynojirimycin (DNJ) from silkworms as functional foods for lower postprandialglucose. International Proceedings of Chemical, Biological and Environmental Engineering. 92: 81–86.
Virk, R.S., Lodhi, G.N. and Ichhponani, J.S. 1980b. Nutritive value of untreated water and acid treated deoiled silkworm pupae meal for broiler chicks. Indian Journal of Poultry Sciences. 15: 155-161.
Wang, H., Hu, W., Liang, Z., Zeng, L., Li, J., Yan, H., Yang, P., Liu, Z. and Wang,L. 2016. Thiol peroxiredoxin, a novel allergen from Bombyx mori,modulates functions of macrophages and dendritic cells. AmericanJournal of Translational Research. 8(12): 5320–5329.
Wei, Z.J., Liao, A.M., Zhang, H.X., Liu, J. and Jiang, S.T. 2009. Optimization of supercritical carbon dioxide extraction of silkworm pupal oil applying the response surface methodology. Bioresource Technology. 100(18): 4214-4219.
Wu, X., He, K., Velickovic, T.C. and Liu, Z. 2021. Nutritional, functional, and allergenic properties of silkworm pupae. Food Science & Nutrition. 9(8): 4655-4665.
Yhoung-Aree, J., Puwastien, P. andAttig, G.A.1997. Edible insects in Thailand: An unconventional protein source. Ecology of Food and Nutrition. 36:133-149.
Zhao, X., Li, L., Kuang, Z., Luo, G. and Li, B. 2015. Proteomic and immunologicalidentification of two new allergens from silkworm (Bombyxmori L.) pupae. Central European Journal of Immunology. 40(1): 30–34.
Zuo, J., Lei, M., Yang, R. and Liu, Z. 2015. Bom m 9 from Bombyx moriisa novel protein related to asthma. Microbiology & Immunology. 59(7):410–418.
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